Isolation ofBurkholderia cenocepaciaandBurkholderia vietnamiensisfrom human sewage

Abstract

Fresh human sewage was examined from a sewage treatment plant for the presence of members of the Burkholderia cepacia complex (BCC) of bacterial organisms and confirmed the presence of viable B. cenocepacia and B. vietnamiensis, by a combination of cultural, phenotypic and genotypic techniques. Both these organisms are important respiratory pathogens for patients with cystic fibrosis (CF). Presently, the survival dynamics of these organisms in sewage effluent and sludge is, as yet, unknown. Therefore, as this study represents the first report of these CF pathogens in sewage and until such survival data is available, careful risk assessment needs to be undertaken in relation to the end use application of potentially contaminated sewage and where such material comes into association with non-colonised patients with cystic fibrosis, so that any potential transmission of these pathogens from sewage to patient is assessed and minimised/eliminated.

Infection Prevention and Control Guideline for Cystic Fibrosis: 2013 Update

“…302 Finally, B. cenocepacia and Burkholderia vietnamiensis have been isolated from human sewage in the United Kingdom, but genotyping of these isolates was not performed. 303 In contrast, the Midwest strain of B. cenocepacia has not been found in the natural environment in regions where this strain infects people with CF. 157,295 P. aeruginosa.…”

The Burkholderia cenocepacia sensor kinase hybrid AtsR is a global regulator modulating quorum‐sensing signalling

“…B. cenocepacia is one of the most prevalent members of the Bcc in CF patients that can be transmitted from patient to patient through social contacts (Govan et al ., 1993) and cause cepacia syndrome, a nearly always fatal condition. Furthermore, B. cenocepacia has been found in association with maize roots (Bevivino et al ., 2011), in sewage (McNeely et al ., 2009), and as the causative agent of banana finger‐tip rot (Lee and Chan, 2007). This bacterium is also virulent in alfalfa, nematodes and insect infection models (Uehlinger et al ., 2009), in zebrafish (Vergunst et al ., 2010), and has the ability to survive within amoebae and macrophages (Lamothe et al ., 2004; 2007).…”

Cangene Gold Medal Award Lecture — Genomic analysis and modification ofBurkholderia cepaciacomplex bacteriophages1This article is based on a presentation by Dr. Karlene Lynch at the 61st Annual Meeting of the Canadian Society of Microbiologists in St. John’s, Newfoundland and Labrador, on 21 June 2011. Dr. Lynch was the recipient of the 2011 Cangene Gold Medal as the Canadian Graduate Student Microbiologist of the Year, an annual award sponsored by Cangene Corporation intended to recogniz

“…Burkholder (Burkholder 1950). Bcc bacteria can be isolated from many locations in the environment, including surface water (Vermis et al 2003), sewage (McNeely et al 2009), plants, and soil/rhizospheres (Burkholder 1950;Gillis et al 1995;Vandamme et al 1997Vandamme et al , 2002Coenye et al 2001; Vanlaere et al 2008Vanlaere et al , 2009. In the past 25 years, it has become clear that Bcc species are not only phytopathogens and environmental species but also opportunistic pathogens.…”