Isolation of human tumor cells that produce cementum proteins in culture

Arzate, H; Olson, S.; Page, Roy C.; Narayanan, A. Sampath

doi:10.1016/0169-6009(92)90796-g

Cited by 44 publications

(30 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…# p \ 0.05 vs. the rh-SHHtreated group differentiation [47]. CAP was identified from a human cementum tumor and proposed to be an extracellular matrix protein functioning in migration and attachment of cementoblast precursors to the root surface [48,49]. CAP was later found to be expressed in PDL cells and alveolar bone cells, and to share homology with some collagen domains [49].…”

Section: Discussionmentioning

confidence: 98%

Sonic Hedgehog Promotes Cementoblastic Differentiation via Activating the BMP Pathways

et al. 2016

View full text Add to dashboard Cite

Although sonic hedgehog (SHH), an essential molecule in embryogenesis and organogenesis, stimulates proliferation of human periodontal ligament (PDL) stem cells, the effects of recombinant human SHH (rh-SHH) on osteoblastic differentiation are unclear. To reveal the role of SHH in periodontal regeneration, expression of SHH in mouse periodontal tissues and its effects on the osteoblastic/cementoblastic differentiation in human cementoblasts were investigated. SHH is immunolocalized to differentiating cementoblasts, PDL cells, and osteoblasts of the developing mouse periodontium. Addition of rh-SHH increased cell growth, ALP activity, and mineralization nodule formation, and upregulated mRNA expression of osteoblastic and cementoblastic markers. The osteoblastic/cementoblastic differentiation of rh-SHH was abolished by the SHH inhibitor cyclopamine (Cy) and the BMP antagonist noggin. rh-SHH increased the expression of BMP-2 and -4 mRNA, as well as levels of phosphorylated Akt, ERK, p38, and JNK, and of MAPK and NF-κB activation, which were reversed by noggin, Cy, and BMP-2 siRNA. Collectively, this study is the first to demonstrate that SHH can promote cell growth and cell osteoblastic/cementoblastic differentiation via BMP pathway. Thus, SHH plays important roles in the development of periodontal tissue, and might represent a new therapeutic target for periodontitis and periodontal regeneration.

show abstract

Section: Discussionmentioning

confidence: 98%

Sonic Hedgehog Promotes Cementoblastic Differentiation via Activating the BMP Pathways

et al. 2016

View full text Add to dashboard Cite

show abstract

“…A 500-mL quantity of conditioned medium was collected from plates of each cell type containing cells at confluent density, and CP was immunopurified as described previously (Arzate et al, 1992b). Protein concentrations were determined by means of the Nano Orange Protein Assay Kit (Molecular Probes, Eugene, OR, USA).…”

Section: Immunoaffinity Chromatography and Immunoblotting Of Cpmentioning

confidence: 99%

“…Protein concentrations were determined by means of the Nano Orange Protein Assay Kit (Molecular Probes, Eugene, OR, USA). Cross-reactivity and expression of CP in human cementoblastoma, periodontal ligament cells, and alveolar bone-derived cells were assessed by immunoblotting as previously described (Arzate et al, 1992b), except that anti-CP antibody was used at a 1:300 dilution. For assessment of the specificity of anti-CP antibody, calfskin type I collagen (Boehringer Mannheim, Germany), bovine FN (Life Technologies, Rockville, MD, USA), and human bone extract were blotted and tested with anti-CP antibody.…”

Section: Immunoaffinity Chromatography and Immunoblotting Of Cpmentioning

confidence: 99%

Immunolocalization of a Human Cementoblastoma-conditioned Medium-derived Protein

et al. 2002

View full text Add to dashboard Cite

Little is known about the molecular mechanisms that regulate the cementogenesis process, because specific cementum markers are not yet available. To investigate whether a cementoblastoma-conditioned medium-derived protein (CP) could be useful as a cementum biological marker, we studied its expression and distribution in human periodontal tissues, human periodontal ligament, alveolar bone, and cementoblastoma-derived cells. In human periodontal tissues, immunoreactivity to anti-CP was observed throughout the cementoid phase of acellular and cellular cementum, cementoblasts, cementocytes, cells located in the endosteal spaces of human alveolar bone, and in cells in the periodontal ligament located near the blood vessels. Immunopurified CP promoted cell attachment on human periodontal ligament, alveolar bone-derived cells, and gingival fibroblasts. A monoclonal antibody against bovine cementum attachment protein (CAP) cross-reacted with CP. These findings indicate that CP identifies potential cementoblast progenitor cells, is immunologically related to CAP species, and serves as a biological marker for cementum.

show abstract

“…A λ-ZAP Express EcoRI/XhoI (Stratagene, La Jolla, CA, USA) cDNA library was constructed with mRNA from human cementum tumor-derived cells (Arzate et al, 1992b). After transient expression in cos-7 cells, it was screened by panning with monoclonal anti-CAP antibody 3G9 (Saito et al, 2001).…”

Section: Isolation Of Cap Cdna and Expression Of Recombinant Proteinmentioning

confidence: 99%

“…Human gingival fibroblasts, cementoblasts, periodontal ligament cells, and alveolar bone cells were obtained as described previously (Arzate et al, 1992b;Pitaru et al, 1995). Attachment activity was determined with human gingival fibroblasts in plates coated with rhPTPLA-CAP (Pitaru et al, 1995;Wu et al, 1996).…”

Section: Cells and Determination Of Attachment Activitymentioning

confidence: 99%

Isolation of Protein-Tyrosine Phosphatase-like Member-a Variant from Cementum

Hoyos

Hoz-Rodríguez²,

Arzate

et al. 2011

J Dent Res

View full text Add to dashboard Cite

A supplemental appendix to this article is published electronically only at http://jdr.sagepub.com/supplemental. AbstrAct Cementum has been shown to contain unique polypeptides that participate in cell recruitment and differentiation during cementum formation. We report the isolation of a cDNA variant for protein-tyrosine phosphatase-like (proline instead of catalytic arginine) member-a (PTPLA) from cementum. A cementifying fibroma-derived λ-ZAP expression library was screened by panning with a monoclonal antibody to cementum attachment protein (CAP), and 1435 bp cDNA (gb AC093525.3) was isolated. This cDNA encodes a 140-amino-acid polypeptide, and its N-terminal 125 amino acids are identical to those of PTPLA. This isoform, designated as PTPLA-CAP, results from a read-through of the PTPLA exon 2 splice donor site, truncating after the second putative transmembrane domain. It contains 15 amino acids encoded within the intron between PTPLA exons 2 and 3, which replace the active site for PTPLA phosphatase activity. The recombinant protein, rhPTPLA-CAP, has Mr 19 kDa and cross-reacts with anti-CAP antibody. Anti-rhPTPLA-CAP antibody immunostained cementum cells, cementum, heart, and liver. Quantitative RT-PCR showed that PTPLA was expressed in all periodontal cells; however, PTPLA-CAP expression was limited to cementum cells. The rhPTPLA-CAP promoted gingival fibroblast attachment. We conclude that PTPLA-CAP is a splice variant of PTPLA, and that, in the periodontium, cementum and cementum cells express this variant.

show abstract

Isolation of human tumor cells that produce cementum proteins in culture

Cited by 44 publications

References 32 publications

Sonic Hedgehog Promotes Cementoblastic Differentiation via Activating the BMP Pathways

Sonic Hedgehog Promotes Cementoblastic Differentiation via Activating the BMP Pathways

Immunolocalization of a Human Cementoblastoma-conditioned Medium-derived Protein

Isolation of Protein-Tyrosine Phosphatase-like Member-a Variant from Cementum

Contact Info

Product

Resources

About