Isolation of human T and B lymphocytes by E-rosette gradient centrifugation. Characterization of the isolated subpopulations

Morphological variants of leukemic cells in B chronic lymphocytic leukemia are associated with different T cell and NK cell abnormalities

“…The different morphological characteristics of typical and atypical leukemic cells may also be associated with potential anti-genic and/or functional differences [5,46]. The existence of activated T lymphocytes with an increased percentage of antigen driven cells and the described preferential use of T cell receptor ␤ chain rearrangement genes in B-CLL patients, is in agreement with this possibility [36,47]. Another explanation is that the different abnormal backgrounds of T lymphocytes and NK cells might allow the preferential expansion and accumulation of different leukemic B cells.…”

“…A decreased percentage of CD4+ T lymphocytes and normal or increased percentage of CD8+ lymphocytes in purified CD2+ populations from these patients have been observed [23][24][25][26][27][28]36]. In this study, the distribution of the CD4 and CD8 subsets in purified CD2+ populations from typical B-CLL and atypical B-CLL patients were seen to be markedly different.…”

Determining the Role of the Epstein-Barr Virus Cp EBNA2-Dependent Enhancer during the Establishment of Latency by Using Mutant and Wild-Type Viruses Recovered from Cottontop Marmoset Lymphoblastoid Cell Lines

“…Partially purified human peripheral B cells were prepared from leukopaks by E-rosette depletion of T cells (13). For each virus stock, 100, 10, 1, and 0.1 l of virus was used to infect 3 ϫ 10 6 human B lymphocytes The bottom diagram represents the targeting plasmid used to incorporate the Cp EBNA2-responsive enhancer deletion into the viral genome.…”

The association between CD2+ peripheral blood lymphocyte subsets and the relapse of bladder cancer in prophylactically BCG-treated patients

“…T-cells were purified by rosetting with sheep red blood cells pretreated with 2-aminoiethylisothiouronium bromide, as previously described (Madsen et al, 1980). After counting, cells were resuspended in RPMI-1640 (Whitaker Bioproducts, Walkersville, USA) supplemented with 10% heatinactivated fetal bovine serum (Biochrom KG, Berlin), L-glutamine (2 mM Biochrom KG, Berlin), Hepes (25 mM Biochrom), and 1% penicillinÐstreptomycin (Difco Lab, Detroit, MI, USA); this will be referred to as complete medium.…”