Isolation of human retinal genes: Recoverin cDNA and gene

Murakami, Akira; Yajima, Toshihiro; Inana, George

doi:10.1016/s0006-291x(05)81483-4

Cited by 59 publications

(31 citation statements)

References 25 publications

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“…Molecular masses of marker proteins (kilodaltons; kDa) are indicated on the left. 1421 &h of recoverin in the cells revealed no alternative splicing and no point mutations in the entire coding region (Murakami et al, 1992). This is in line with the above-mentioned animal model, in which the uveoretinitis could be experimentally induced by a purified wild-type form of recoverin.…”

Section: Discussionsupporting

confidence: 86%

“…This primer pair was designed to amplify the recoverin gene between exon 1 and exon 3 of the published human recoverin sequence (Murakami et al, 1992). The primers used for amplification from human recoverin sequence were 5'-TGT GTT CCG CAG CTT CGA TT-3'(sense) and 5'-TGA GGC TCA AAC TGG ATC AG-3'(antisense).…”

Section: Cell Linesmentioning

confidence: 99%

“…The membrane was washed twice with the same solution for 30 min, and incubated for 1 h with a 10 000-fold diluted alkaline phosphatase-conjugated goat anti-rabbit IgG (Organon Teknika, Durham, NC, USA) in buffer containing 1% bovine serum albumin (BSA). After rinsing, blots were developed using a chemiluminescence reaction (LumiPhos 530, Boehringer Mannheim), then exposed to radiographic film for 15 (Murakami et al, 1992).…”

Section: Cell Linesmentioning

confidence: 99%

See 2 more Smart Citations

Expression of a photoreceptor protein, recoverin, as a cancer-associated retinopathy autoantigen in human lung cancer cell lines

Matsubara

Yamaji²,

Sato³

et al. 1996

Br J Cancer

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Section: Discussionsupporting

confidence: 86%

Section: Cell Linesmentioning

confidence: 99%

Section: Cell Linesmentioning

confidence: 99%

See 1 more Smart Citation

Expression of a photoreceptor protein, recoverin, as a cancer-associated retinopathy autoantigen in human lung cancer cell lines

Matsubara

Yamaji²,

Sato³

et al. 1996

Br J Cancer

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“…According to the reported sequences of the proteins shown, evolutionary distances of the sequences were calculated. The sequences used were those of visinin (13), S-modulin (7), bovine recoverin (5), human recoverin (43), and mouse recoverin (44). Bar indicates 10% replacement of an amino acid per site (k ϭ 0.1; see "Experimental Procedures").…”

Section: Rod-and-cone Cell-type Specific Expression Of S-modulin Homomentioning

confidence: 99%

Photoreceptor Protein s26, a Cone Homologue of S-modulin in Frog Retina

Kawamura

Kuwata

Yamada

et al. 1996

Journal of Biological Chemistry

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A frog retinal protein named s26 is a 26-kDa protein found during purification of S-modulin in frog retina (Kawamura, S. (1992) Photochem. Photobiol. 56, 1173-1180). To identify its role in frog retina, first s26 was purified to nearly homogeneity with three chromatographical steps. Based on the partial amino acid sequences of the proteolysed fragments of s26, we isolated cDNAs that encode s26. The analysis of its amino acid sequence revealed that s26 is an S-modulin-like protein, while it shows higher homology to visinin. Visinin is a Ca 2؉ -binding protein reported to be present in chicken cones, but its localization in the retina had been a subject in dispute. The present study showed that s26 is present in cone photoreceptors. The study also showed that s26 inhibits phosphorylation of rhodopsin after a light flash at high Ca 2؉ concentrations as S-modulin does. From these results, we concluded that s26 is a cone homologue of S-modulin. The result is consistent with the idea that each type of photoreceptors expresses each cell-type specific version of phototransduction proteins.

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confidence: 99%

X‐Arrestin: a new retinal arrestin mapping to the X chromosome

et al. 1993

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We have been using a differential cDNA cloning approach to isolate human retina-specific and retina-enriched genes [1]. A 1,314 bp cDNA was isolated by this approach, representing a highly retina-specific message encoding a 388 amino acid protein showing 58%, 50%, and 49% homology to bovine fl-arrestin, and bovine and human retinal arrestin (S-antigen), respectively. Chromosomal mapping localized this new arrestin gene to the proximal long arm of the X chromosome, hence it was named X-arrestin. In situ hybridization demonstrated its expression in the inner and outer segments and the inner plexiform regions of the retina.

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Isolation of human retinal genes: Recoverin cDNA and gene

Cited by 59 publications

References 25 publications

Expression of a photoreceptor protein, recoverin, as a cancer-associated retinopathy autoantigen in human lung cancer cell lines

Expression of a photoreceptor protein, recoverin, as a cancer-associated retinopathy autoantigen in human lung cancer cell lines

Photoreceptor Protein s26, a Cone Homologue of S-modulin in Frog Retina

X‐Arrestin: a new retinal arrestin mapping to the X chromosome

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