Isolation of human mesenchymal stem cells from third‐trimester amniotic fluid

You, Qi; Cai, Lu; Zheng, Jianhua; Tong, Xiaojing; Zhang, Dan; Zhang, Yuanlong

doi:10.1016/j.ijgo.2008.06.012

Cited by 69 publications

(53 citation statements)

References 8 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, these amniotic fluid stem (AFS) cells were not tumorigenic, retained long telomeres, and maintained a normal karyotype for over 250 population doublings (De Coppi et al, 2007). The presence of NANOG protein and SSEA-4 in human AFS cells (Roubelakis et al, 2007), their differentiation into chondrocytes (Kim et al, 2007;Kolambkar et al, 2007) and osteocytes (You et al, 2008), and the presence of telomerase activity (Kim et al, 2007) have been reported. It seems therefore that AFS cells are pluripotent stem cells similar to -but less differentiated -than BM-MSCs (Kim et al, 2007).…”

Section: Amniotic Fluid Derived Cellsmentioning

confidence: 99%

“…AFCs can be collected during the second trimester of gestation by amniocentesis. Amniotic fluid is rich in mesenchymal stem cells (Fauza, 2004;Siegel et al, 2008) that possess high proliferation potential, are OCT4 positive; and are able to differentiate into cells types of the three germ layers, like adipogenic, osteogenic, myogenic and endothelial cell types, neurogenic cell types, and hepatic cell types (De Coppi et al, 2007;You et al, 2008;Zheng et al, 2008). A recent study reported that c-Kit+ Lin-cells derived from human amniotic fluid displayed a multilineage differentiation potential in vitro to the haematopoietic lineages (Ditadi et al, 2009).…”

Section: Amniotic Fluid Derived Cellsmentioning

confidence: 99%

See 1 more Smart Citation

On the origin of amniotic stem cells: of mice and men

Dobreva¹,

Pereira²,

Deprest³

et al. 2010

Int. J. Dev. Biol.

135

139

View full text Add to dashboard Cite

A common characteristic of mammals is the development of extraembryonic supporting tissues and organs that are required for embryonic implantation, survival and development in utero. The amnion is the innermost extraembryonic membrane that eventually surrounds the fetus of amniotes, and contains the amniotic fluid. Next to its function in in utero development, the amnion has been shown to have an important potential for clinical applications. It is mainly used as a dressing to stimulate healing in skin and ocular wounds. Moreover, cells derived from the amniotic membrane and amniotic fluid have been reported to possess stem cell features, like pluripotent differentiation ability. Little is known about the early development of this membrane in humans. The mouse is a powerful genetic model organism that can be used to address the dynamics and the developmental origin of amnion and amnion-derived stem cells. Here, we discuss some fundamental differences in amnion development in the disc-shaped primate embryo and in the cup-shaped mouse embryo. We emphasize the consequences that this may have on the derivation of amniotic "stem" cells. After revision of the different isolation procedures of amniotic (fluid) derived "stem" cells from rodents, we reveal striking differences in the sources used to derive these cells across studies. The profound differences in the development of the extraembryonic membranes and cavities between primates and rodents may result in comparing cell types of different developmental origins, eventually leading to missinterpretations.

show abstract

Section: Amniotic Fluid Derived Cellsmentioning

confidence: 99%

Section: Amniotic Fluid Derived Cellsmentioning

confidence: 99%

On the origin of amniotic stem cells: of mice and men

Dobreva¹,

Pereira²,

Deprest³

et al. 2010

Int. J. Dev. Biol.

135

139

View full text Add to dashboard Cite

show abstract

“…However, due to their adipogenic, osteogenic, myogenic, endothelial, neurogenic, hepatic, chondrogenic and renal differentiation potency (Tsai et al 2004, AFMSCs may be a readily available source similar to ESCs for large numbers of different cells progenitors (De Coppi et al 2007). In addition, AFMSCs have not shown tumorigenicity, thus making them an exciting new source of regenerative cells (You et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Isolation, characterization and differentiation of mesenchymal stem cells from amniotic fluid, umbilical cord blood and Wharton's jelly in the horse

Iacono

Brunori²,

Pirrone³

et al. 2012

Reproduction

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) have been derived from multiple sources of the horse including umbilical cord blood (UCB) and amnion. This work aimed to identify and characterize stem cells from equine amniotic fluid (AF), CB and Wharton's Jelly (WJ). Samples were obtained from 13 mares at labour. AF and CB cells were isolated by centrifugation, while WJ was prepared by incubating with an enzymatic solution for 2 h. All cell lines were cultured in DMEM/TCM199 plus fetal bovine serum. Fibroblast-like cells were observed in 7/10 (70%) AF, 6/8 (75%) CB and 8/12 (66.7%) WJ samples. Statistically significant differences were found between cell-doubling times (DTs): cells isolated from WJ expanded more rapidly (2.0G0.6 days) than those isolated from CB (2.6G1.3 days) and AF (2.3G1.0 days) (P!0.05). Positive von Kossa and Alizarin Red S staining confirmed osteogenesis. Alcian Blue staining of matrix glycosaminoglycans illustrated chondrogenesis and positive Oil Red O lipid droplets staining suggested adipogenesis. All cell lines isolated were positive for CD90, CD44, CD105; and negative for CD34, CD14 and CD45. These findings suggest that equine MSCs from AF, UCB and WJ appeared to be a readily obtainable and highly proliferative cell lines from a uninvasive source that may represent a good model system for stem cell biology and cellular therapy applications in horses. However, to assess their use as an allogenic cell source, further studies are needed for evaluating the expression of markers related to cell immunogenicity.

show abstract

“…Furthermore, characteristics of MSCs are the absence of expression of typical hematopoietic antigens like CD34 and CD45, and the expression of surface markers like Stro-1, CD44, CD73, CD90, CD105 and CD166 (Pittenger et al, 1999). Human MSCs, which are probably responsible for normal tissue renewal, as well as for response to injury (Tsai et al, 2007), have been isolated from several tissues, including bone marrow (Kastrinaki et al, 2008;Yoo et al, 1998), periosteum (Nakahara et al, 1990), perichondrium (Dounchis et al, 1997) (You et al, 2008;Steigman & Fauza, 2007;Fauza, 2004), placenta (Barlow et al, 2008, Steigman & Fauza, 2007Fauza, 2004: Matikainen & Laine, 2005, amniotic membrane (Díaz-Prado et al, 2010a& 2010bAlviano et al, 2007), umbilical cord (Baksh et al, 2007 and umbilical cord blood (Mareschi et al, 2001). Although bone marrow is the usual source of MSCs, umbilical cord blood is emerging as an important reservoir for stem cells capable of differentiation into many cell types and possessing the advantages of immune status and relatively unshortened telomere length (McGuckin et al, 2005).…”

Section: Mesenchymal Stem Cells Transplantationmentioning

confidence: 99%