Isolation of Human Enteroviruses from Beagle Dogs

Abstract

level. We are currently investigating the effects of HE on synthesis of specific protein hormones in an organ culture system. Summary. Rat anterior pituitary glands cultured for periods up to four hours incorporate amino acid-14C mixture or glutamic acid-14C into pituitary protein in linear fashion. Addition of hypothalamic extracts to the culture system results in an increased incorporation and release of protein radioactivity as compared to glands incubated with cerebral cortex extracts. The stimulatory effect can be demonstrated at the level of the pituitary polyribosomes.

Antiviral Antibodies in Dogs in the Netherlands

“…Serological surveys are usually performed with the intention to show the incidence of selected infectious agents in a geographically defined host population. For canine viruses, surveys have been performed in several countries ( 2 , 6, 10, 11, 14,16,20). The resent study is the first of this kind for the In our collection of sera sampled from dogs, antibodies to CAV, are found more frequently and with higher levels in sera from the OP.…”

“…The role played by the dog in the epizootiology of viruses occurring in other species is still unknown. Serologic surveys have been carried out in several countries in order to estimate the incidence of different virus infections in the dog (2, 6, 10,11,14,16,20). As in other countries infectious laryngotracheitis (kennel cough) and neonatal mortality (NM) represent unresolved problems in the Netherlands, too.…”

A survey for human enteroviruses in dogs and man

“…It was observed that the enteroviruses previously recovered occurred less frequently in each successive group of Beagle dogs sampled (4,6). In previous surveys (4, 6) such viruses were isolated repeatedly from Beagle dogs in the same facilities.…”

“…The procedures followed were the same as those used previously (4). Essentially, primary rhesus monkey kidney (MK) cells 2 and HeLa cells were grown under medium 199 with 10% calf serum and antibiotics (500 units of penicillin G sodium, 100 ~g of streptomycin sulfate, 100 ~g of neomycin sulfate and 5 ~g of Fungizonc/ml of medium).…”

“…We previously reported the occurrence of human enteroviruses in Beagle dogs maintained in the Lovelace Foundation Fission Product Inhalation Program (FPIP) kennels (4,6). In these studies, conducted between 1961 and 1966, a total of 187 isolates of ECHO virus 6 and coxsackieviruses B1, B3, and B5 were made from 1645 rectal, nasal and throat swabs collected from 69 healthy one to two year old Beagle dogs.…”