Isolation of human adult olfactory sphere cells as a cell source of neural progenitors

Ohnishi, Yozo; Iwatsuki, Koichi; Ishihara, Masahiro; Shikina, Takashi; Shinzawa, Koei; Moriwaki, Takashi; Ninomiya, Koshi; Ohkawa, Taka-aki; Umegaki, Masao; Kishima, Haruhiko; Yoshimine, Toshiki

doi:10.1016/j.scr.2015.04.006

Cited by 15 publications

(13 citation statements)

References 21 publications

(29 reference statements)

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“…The olfactory mucosa has multipotent horizontal basal cells that differentiate into olfactory sensory neurons during normal neuronal turnover as well as during tissue regeneration after injury . To date, several studies have described different techniques that olfactory stem cells are generated from olfactory mucosa, using various culture conditions according to the species or the presence of serum . However, the culture methods and cellular identity of olfactory stem cells have not been settled.…”

Section: Discussionmentioning

confidence: 99%

“…However, the culture methods and cellular identity of olfactory stem cells have not been settled. Among those, some studies demonstrated that olfactory mucosa generated neurospheres that were multipotent in vitro and human olfactory neurospheres gave rise to neurons and glia, and self‐replicating . OSCs are a kind of cluster for stem cells generated from olfactory mucosa in suspension culture, and these cells express markers of neural stem cells, glial cells, neurons and mesenchymal cells.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we generated olfactory sphere cells (OSCs) in cultures that were derived from mouse olfactory mucosa as previously described and showed that these OSCs had characteristics of neural stem cell (NSC). We also developed a murine model with allergic inflammation, in which all mice showed olfactory dysfunction such as anosmia or hyposmia.…”

Section: Introductionmentioning

confidence: 99%

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Tumour necrosis factor alpha and interleukin‐5 inhibit olfactory regeneration via apoptosis of olfactory sphere cells in mice models of allergic rhinitis

Kim

Choi

Eun

et al. 2019

Clin Experimental Allergy

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Background: Olfactory dysfunction is frequently experienced by patients with allergic rhinitis. It is thought to result from structural and functional changes occurring in the olfactory mucosa caused by inflammation. However, the current understanding of the pathophysiology of olfactory dysfunction in allergic rhinitis remains unclear.Objective: To investigate the mechanism by which the olfactory neural cells are damaged in allergic rhinitis. Methods:Olfactory sphere cells (OSCs) were established after dissociation and serial cultures of cells from the mouse olfactory mucosa. Viability and proliferation of OSCs were compared between control and allergic rhinitis mice models, and olfactory stem cell markers were analysed in vivo. To elucidate which cytokines have an inhibitory effect on OSCs, viability and apoptotic markers of OSCs were investigated.Results: Olfactory sphere cells were successfully isolated from the olfactory mucosa of mice, and these cells expressed markers of neural stem cells. To investigate the neural differentiation, we performed the immunocytochemical staining and found significantly elevated expressions of Tuji1, GFAP and O4 on OSCs. On the comparison of the characteristics of OSCs between control and allergic rhinitis model, we detected significantly fewer neurospheres, reduced clonogenic capacity and decreased expression of olfactory neural stem cell markers in allergic rhinitis model. When OSCs were treated with several major allergic cytokines were treated on OSCs, only TNF-α showed an inhibitory effect on OSCs. Interestingly, IL-5 had an inhibitory effect on the viability of OSCs in combination with TNF-α, whereas IL-5 alone does not have an effect. Moreover, TNF-α combined with IL-5 significantly increased the apoptotic expression, compared with TNF-α or IL-5 alone. Additionally, allergic rhinitis mice models showed the increased apoptotic expression. Conclusion and Clinical Relevance:Allergic rhinitis mice models showed lower expression of OSCs, and TNF-α combined with IL-5 had an apoptotic effect on OSCs.Therefore, these cytokines may be therapeutic targets for olfactory dysfunction in patients with allergic rhinitis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tumour necrosis factor alpha and interleukin‐5 inhibit olfactory regeneration via apoptosis of olfactory sphere cells in mice models of allergic rhinitis

Kim

Choi

Eun

et al. 2019

Clin Experimental Allergy

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“…Similarly, culture techniques have been established for human OE to generate populations of mitotically active neural progenitors that form neurospheres [28, 29, 31]. The resulting olfactory neurospheres and their derived cells are multipotent and capable of self-renewal, and can be differentiated into neurons and glia [29, 32].…”

Section: Oe-derived Tissue/cell Modelsmentioning

confidence: 99%

“…Indeed, olfactory lamina propria stem cells share membrane markers with bone marrow stem cells, and are named, olfactory ectomesenchymal stem cells [37]. Other groups have demonstrated that neurosphere-forming cells derived from the OE can be directed toward neuronal lineage restriction by sonic hedgehog, forskolin, and retinoic acid [38], and that human olfactory spheres express oligodendrocyte progenitor cell markers [31]. …”

Section: Oe-derived Tissue/cell Modelsmentioning

confidence: 99%

Application of olfactory tissue and its neural progenitors to schizophrenia and psychiatric research

Lavoie

Sawa²,

Ishizuka³

2017

Current Opinion in Psychiatry

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Purpose of review The goal of this review article is to introduce olfactory epithelium (OE)-derived cell/tissue models as a promising surrogate system to study the molecular mechanisms implicated in schizophrenia (SZ) and other neuropsychiatric disorders. Here we particularly focus the utility of their neural progenitors. Recent findings Recent investigations of the pathophysiology of SZ using OE-derived tissue/cell models have provided insights about SZ-associated alterations in neurodevelopment, stress response, and gene/protein expression regulatory pathways. Summary The OE retains the capacity for lifelong neurogenesis and regeneration, because of the presence of neural stem cells and progenitors. Thus, both mature neurons and neural progenitors can be obtained from the OE without the need for genetic reprogramming and related confounds. Furthermore, the OE is highly scalable resource in translational settings. Here we also demonstrate recent findings from research using OE-derived tissue/cell models in SZ and other brain disorders. In summary, we propose that the OE as a promising resource to study neural molecular and cellular signatures relevant to the pathology of SZ and other mental disorders.

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Functional Consequences of Keratan Sulfate Sulfation in Electrosensory Tissues and in Neuronal Regulation

Melrose

2019

Adv. Biosys.

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Keratan sulfate (KS) is a functional electrosensory and neuro‐instructive molecule. Recent studies have identified novel low sulfation KS in auditory and sensory tissues such as the tectorial membrane of the organ of Corti and the Ampullae of Lorenzini in elasmobranch fish. These are extremely sensitive proton gradient detection systems that send signals to neural interfaces to facilitate audition and electrolocation. High and low sulfation KS have differential functional roles in song learning in the immature male zebra song‐finch with high charge density KS in song nuclei promoting brain development and cognitive learning. The conductive properties of KS are relevant to the excitable neural phenotype. High sulfation KS interacts with a large number of guidance and neuroregulatory proteins. The KS proteoglycan microtubule associated protein‐1B (MAP1B) stabilizes actin and tubulin cytoskeletal development during neuritogenesis. A second 12 span transmembrane synaptic vesicle associated KS proteoglycan (SV2) provides a smart gel storage matrix for the storage of neurotransmitters. MAP1B and SV2 have prominent roles to play in neuroregulation. Aggrecan and phosphacan have roles in perineuronal net formation and in neuroregulation. A greater understanding of the biology of KS may be insightful as to how neural repair might be improved.

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Isolation of human adult olfactory sphere cells as a cell source of neural progenitors

Cited by 15 publications

References 21 publications

Tumour necrosis factor alpha and interleukin‐5 inhibit olfactory regeneration via apoptosis of olfactory sphere cells in mice models of allergic rhinitis

Tumour necrosis factor alpha and interleukin‐5 inhibit olfactory regeneration via apoptosis of olfactory sphere cells in mice models of allergic rhinitis

Application of olfactory tissue and its neural progenitors to schizophrenia and psychiatric research

Functional Consequences of Keratan Sulfate Sulfation in Electrosensory Tissues and in Neuronal Regulation

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