Isolation of HIV-1 From Vitreous Humor

Srinivasan, Arjun; Kalyanaraman, S.; Dutt, Kamla; Butler, D Jr; Kaplan, H. J.

doi:10.1016/0002-9394(89)90022-6

Cited by 8 publications

(1 citation statement)

References 5 publications

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“…1-3 HIV-1 genome, antigens, or infectious virus were found in corneas, 3 tears, 4 conjunctival epithelium, 5 retina, 6,7 and vitreous [8][9][10][11][12] from AIDS patients. The most common ocular condition of HIV-1 infection is a microangiopathy , also known as retinal microvasculopat hy or AIDS retinopathy, which is characterized by cotton-wool spots and, less frequently, intraretinal hem orrhages.…”

Section: Introduction T H E Ey E Is a C O M M O N Site Of Neuropatholmentioning

confidence: 99%

Human Immunodeficiency Virus Type 1 Can Infect Human Retinal Pigment Epithelial Cells in Culture and Alter the Ability of the Cells to Phagocytose Rod Outer Segment Membranes

Canki

Sparrow²,

Chao³

et al. 2000

AIDS Research and Human Retroviruses

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Human immunodeficiency virus type 1 (HIV-1) has been found in the vitreous of persons with AIDS. Here we investigated the susceptibility of human retinal pigment epithelial (RPE) cells to HIV-1 infection in culture and the effects of HIV-1 on the phagocytic function of the RPE. We found that 10 of 11 populations of RPE cells isolated from different fetal or adult eyes were susceptible to low-level replication of HIV-1/NL4-3 as determined by the detection of viral DNA and spliced viral RNA encoding envelope. HIV-1 infection was not inhibited by recombinant soluble CD4, suggesting that CD4 is not required for virus entry into RPE cells. RPE cells fused with target cells constitutively expressing HIV-1 envelope glycoproteins, indicating that HIV-1 enters cells by receptor-mediated fusion. Exposure to HIV-1 or recombinant gp120 caused a two- to four-fold increase in the binding and uptake of isolated rod outer segments by RPE cells. These findings introduce a new cell target of HIV-1 replication in the eye and indicate that RPE cells function aberrantly when exposed to HIV-1 or its envelope glycoprotein.

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Section: Introduction T H E Ey E Is a C O M M O N Site Of Neuropatholmentioning

confidence: 99%

Human Immunodeficiency Virus Type 1 Can Infect Human Retinal Pigment Epithelial Cells in Culture and Alter the Ability of the Cells to Phagocytose Rod Outer Segment Membranes

Canki

Sparrow²,

Chao³

et al. 2000

AIDS Research and Human Retroviruses

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Hiv

London¹,

Cunningham²

2016

Intraocular Inflammation

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Detection of HIV in human vitreous

et al. 1993

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Assay of human vitreous specimens obtained postmortem for HIV antibodies, or HIV p24 antigen, is reported to be a reliable technique to demonstrate HIV infection in possible cornea donors from whom serum could not be obtained. We tested three vitreous samples obtained during vitrectomy from two HIV-positive patients. One patient exhibited the clinical AIDS syndrome. HIV antigen and antibody tests were negative in all specimens. HIV proviral DNA was detected by PCR only in the vitreous of the patient with AIDS. Therefore, testing only vitreous samples is insufficient to exclude HIV infection in potential cornea donors.

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Isolation of HIV-1 From Vitreous Humor

Cited by 8 publications

References 5 publications

Human Immunodeficiency Virus Type 1 Can Infect Human Retinal Pigment Epithelial Cells in Culture and Alter the Ability of the Cells to Phagocytose Rod Outer Segment Membranes

Human Immunodeficiency Virus Type 1 Can Infect Human Retinal Pigment Epithelial Cells in Culture and Alter the Ability of the Cells to Phagocytose Rod Outer Segment Membranes

Hiv

Detection of HIV in human vitreous

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