Isolation of Histophilus somni from the nasal exudates of a clinically healthy adult goat

Pérez-Romero, Nelson; Aguilar-Romero, Francisco; Arellano-Reynoso, Beatriz; Díaz-Aparicio, Efrén; Hernández‐Castro, Rigoberto

doi:10.1007/s11250-011-9792-6

Cited by 7 publications

(6 citation statements)

References 9 publications

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“…Histophilosis or the HSDC, occurs predominantly in cattle with sporadic reports of disease occurring in several species of small ruminants. This bacterium has been isolated from healthy goats in Mexico 6 and Hungary 7 and in sheep from Ethiopia. 8 Reports of H. somni associated diseases in sheep include TME, 9 , 10 , 11 pneumonia, 9 reproductive disorders, 12 and endometritis 9 , 13 ; spontaneous 14 and experimentally induced epididymitis 15 have also been described.…”

Section: Introductionmentioning

confidence: 99%

Histophilus somni -associated syndromes in sheep from Southern Brazil

Headley

Pereira

Balbo

et al. 2018

Brazilian Journal of Microbiology

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Histophilus somni is a Gram-negative bacterium that is associated with a disease complex (termed histophilosis) that can produce several clinical syndromes predominantly in cattle, but also in sheep. Histophilosis is well described in North America, Canada, and in some European countries. In Brazil, histophilosis has been described in cattle with respiratory, reproductive, and systemic disease, with only one case described in sheep. This report describes the occurrence of Histophilus somni-associated disease in sheep from Southern Brazil. Eight sheep with different clinical manifestations from five farms were investigated by a combination of pathological and molecular diagnostic methods to identify additional cases of histophilosis in sheep from Brazil. The principal pathological lesions were thrombotic meningoencephalitis, fibrinous bronchopneumonia, pulmonary abscesses, and necrotizing myocarditis. The main clinical syndromes associated with H. somni were thrombotic meningoencephalitis (n = 4), septicemia (n = 4), bronchopneumonia (n = 4), and myocarditis (n = 3). H. somni DNA was amplified from multiple tissues of all sheep with clinical syndromes of histophilosis; sequencing confirmed the PCR results. Further, PCR assays to detect Pasteurella multocida and Mannheimia haemolytica were negative. These findings confirmed the participation of H. somni in the clinical syndromes investigated during this study, and adds to the previous report of histophilosis in sheep from Brazil.

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Section: Introductionmentioning

confidence: 99%

Histophilus somni -associated syndromes in sheep from Southern Brazil

Headley

Pereira

Balbo

et al. 2018

Brazilian Journal of Microbiology

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“…Although H. somni-related diseases are of economic importance to livestock owners (George 2009), clinical manifestations of disease in cattle is predominant within the USA (Fecteau and George 2004;Woolums et al 2009) and Canada (Van Donkersgoed et al 1990;Gagea et al 2006), with reports of sporadic outbreaks occurring in Europe (Tegtmeier et al 1999;Janosi et al 2009b), Argentina (Descarga et al 2002), Nigeria (Odugbo et al 2009), Japan (Momotani et al 1985;Tanaka et al 2005), and South Africa (Last et al 2001); there is no description of this pathogen affecting cattle in Brazil. Nevertheless, H. somni has also been isolated from healthy goats in Mexico (Perez-Romero et al 2011) and Hungary (Janosi et al 2009a) and has been associated TME in sheep within Europe (Cassidy et al 1997), reproductive disease in sheep from Canada (Lees et al 1990), and in one case of endometritis in a sheep from Brazil (Rizzo et al 2012). Furthermore, H. somni-induced infections are more predominant in young cattle (Harris and Janzen 1989;Van Donkersgoed et al 1990) and in feedlot relative to dairy and pastured animals (Fecteau and George 2004), occurring within the first 2 weeks or months of the feeding period (Woolums et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Histophilus somni-induced infections in cattle from southern Brazil

Headley

Oliveira

Figueira

et al. 2013

Trop Anim Health Prod

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The sudden death of three calves, one diarrheic calf, and one aborted fetus from four farms in southern Brazil was investigated. Two Histophilus somni-associated syndromes were identified: systemic histophilosis (n = 4) and abortion (n = 1). The principal pathological findings included vasculitis, meningoencephalitis with thrombosis, necrotizing myocarditis, renal infarctions, hepatic abscesses, and bronchopneumonia. PCR assays were used to amplify specific amplicons of the ovine herpesvirus 2, bovine herpesvirus 1 and -5, Listeria monocytogenes, H. somni, and pestivirus; bovine group A rotavirus (BoRV-A) and bovine coronavirus (BCoV) were investigated in calves with diarrhea. H. somni DNA was amplified in tissues from all calves and the brain of the aborted fetus with pathological alterations consistent with histophilosis. All other PCR assays were negative; BoRV-A and BCoV were not identified. These findings confirm the participation of H. somni in the pathological alterations observed in this study and represent the first description of histophilosis in cattle from Brazil.

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“…In cattle, H. somni has been associated with bronchopneumonia, myocarditis, infectious thrombotic meningoencephalitis, arthritis, mastitis, and spontaneous abortion (17)(18)(19)(20)(21)(22). It has also been associated with disease in domestic sheep, bighorn sheep, and bison (20,22,23) and has been isolated from the nasal and reproductive tracts of goats (24,25). The Tbp system is likely to be the receptor that H. somni uses to take up iron during asymptomatic colonization of the host, since the targets of its other iron receptors, such as heme or hemoglobin, are normally intracellular.…”

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Transferrin Binding Protein B and Transferrin Binding Protein A2 Expand the Transferrin Recognition Range of Histophilus somni

Pogoutse

Moraes

2020

J Bacteriol

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The bacterial bipartite transferrin receptor is an iron acquisition system that several important human and animal pathogens require for survival. It consists of the TonB-dependent transporter transferrin binding protein A (TbpA) and the surface lipoprotein transferrin binding protein B (TbpB). Curiously, the Tbps are only found in host-specific pathogens and are themselves host specific, meaning that they will bind to the transferrin of their host species but not to the transferrins of other animal species. While this phenomenon has long been established, neither the steps in the evolutionary process that led to this exquisite adaptation for the host nor the steps that could alter it are known. We sought to gain insight into these processes by studying Tbp specificity in Histophilus somni, an economically important pathogen of cattle. A past study showed that whole cells of H. somni specifically bind bovine transferrin but not transferrin from sheep and goats, two bovids whose transferrins share 93% amino acid sequence identity with bovine transferrin. To our surprise, we found that H. somni can use sheep and goat transferrins as iron sources for growth and that HsTbpB, but not HsTbpA, has detectable affinity for sheep and goat transferrins. Furthermore, a third transferrin binding protein found in H. somni, HsTbpA2, also showed affinity for sheep and goat transferrins. Our results suggest that H. somni TbpB and TbpA2 may contribute to broadening the host transferrin recognition range of H. somni. IMPORTANCE Host-restricted pathogens infect a single host species or a narrow range of host species. Histophilus somni, a pathogen that incurs severe economic losses for the cattle industry, infects cattle, sheep, and goats but not other mammals. The transferrin binding proteins, TbpA and TbpB, are thought to be a key iron acquisition system in H. somni; however, despite their importance, H. somni TbpA and TbpB were previously shown to be cattle transferrin specific. In our study, we find that H. somni TbpB and another little-studied Tbp, TbpA2, bind sheep and goat transferrins, as well as bovine transferrin. Our results suggest that TbpB and TbpA2 may allow for host range expansion and provide a mechanism for how host specificity in Tbp-encoding pathogens can be altered.

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Isolation of Histophilus somni from the nasal exudates of a clinically healthy adult goat

Cited by 7 publications

References 9 publications

Histophilus somni -associated syndromes in sheep from Southern Brazil

Histophilus somni -associated syndromes in sheep from Southern Brazil

Histophilus somni-induced infections in cattle from southern Brazil

Transferrin Binding Protein B and Transferrin Binding Protein A2 Expand the Transferrin Recognition Range of Histophilus somni

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