“…Protocols using these core methods have been shown to produce high‐purity histone extractions and high‐quality MS data in several model organisms, including humans, mice, flies, and worms (Garcia et al., 2007; Haws et al., 2020; Holt et al., 2021; L. Johnson, 2004; Jung et al., 2010; Karch et al., 2013; Shechter et al., 2007; Sidoli, Simithy, Karch, Kulej, & Garcia, 2015; Zhou et al., 2017). However, most protocols for histone extraction have been designed for non‐plant organisms and produce low‐purity histones or low‐quality data when used in plants, evidenced by the requirement for additional purification steps, such as gel excision and chromatography, or the use of membranes to remove non‐histone proteins and other impurities in existing plant‐based methods (Chen et al., 2015; L. Johnson, 2004; Ledvinová et al., 2018; Moraes et al., 2015; Zhou et al., 2021). Additional purification steps complicate the overall workflow and extend the required time to purify histones when compared to protocols used in other organisms.…”