Isolation of haptenic material from ragweed pollen

Attallah, N.A.; Sehon, A.H.

doi:10.1016/0019-2791(69)90199-2

Cited by 23 publications

(18 citation statements)

References 9 publications

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“…The IgE reactivity of the allergen extracts was reduced by denaturation using aldehydes resulting in allergoids [69,70,77,78], by proteolysis [79][80][81] or by conjugation of the extracts to chemical groups (e.g. using mPEG) [82,83].…”

Section: Past and Present Attempts For The Improvement Of Allergen-spmentioning

confidence: 99%

Developments in allergen‐specific immunotherapy: from allergen extracts to allergy vaccines bypassing allergen‐specific immunoglobulin E and T cell reactivity

Focke

Swoboda

Marth

et al. 2010

Clin Experimental Allergy

101

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SummaryAllergen-specific immunotherapy (SIT) is the only specific and disease-modifying approach for the treatment of allergy but several disadvantages have limited its broad applicability. We argue that the majority of the possible disadvantages of SIT such as unwanted effects, poor efficacy and specificity as well as inconvenient application are related to the poor quality of natural allergen extracts, which are the active ingredients of all currently available allergy vaccines. Because of the progress made in the field of molecular allergen characterization, new allergy vaccines based on recombinant allergens, recombinant hypoallergenic allergen derivatives and allergen-derived T cell peptides have entered clinical testing and hold promise to reduce the side-effects and to increase the specificity as well as the efficacy of SIT. Here, we present a refined immunotherapy concept, which is based on the use of peptides derived from allergen surfaces that exhibit reduced, allergen-specific IgE as well as T cell reactivity. These peptides when fused to non-allergenic carriers give rise to allergen-specific protective IgG responses with T cell help from a non-allergenic carrier molecule. We summarize the experimental data demonstrating that such peptide vaccines can bypass allergen-specific IgE as well as T cell activation and may be administered at high doses without IgE-and T cell-mediated side-effects. Should these peptide vaccines prove efficacious and safe in clinical trials, it may become possible to develop convenient, safe and broadly applicable forms of SIT as true alternatives to symptomatic, drug-based allergy treatment.

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Section: Past and Present Attempts For The Improvement Of Allergen-spmentioning

confidence: 99%

Developments in allergen‐specific immunotherapy: from allergen extracts to allergy vaccines bypassing allergen‐specific immunoglobulin E and T cell reactivity

Focke

Swoboda

Marth

et al. 2010

Clin Experimental Allergy

101

View full text Add to dashboard Cite

show abstract

“…On the other hand, penicillin al lergy, which is triggered by this chemically well-defined antibiotic, has been managed by inhibiting the reaction between the tissue fixed anti-penicillin reagins and the corre sponding multivalent antigen(s) by the ad ministration of an appropriate univalent penicillin derivative, benzylpenicilloylformyl-L-lysine [6]. Similarly, it was dem onstrated earlier in this laboratory that the aqueous extract of ragweed pollen con tained hapten-like constituents capable of inhibiting the Prausnitz-Kiistner reaction, which was inducible with the high molecular weight multivalent components of ragweed pollen [1]. Unfortunately, however, due to the unavailability of haptenic materials from pollen in sufficient amounts, this tech nique of inhibition of allergic reactions by saturating IgE antibodies on target tissues with the hapten-like components present in pollens has not been exploited on a wide scale.…”

Section: Introductionmentioning

confidence: 99%

Modulation of the IgE Antibody Response in Rats to Kentucky Blue Grass Pollen Allergens

Ekramoddoullah¹,

Kisil²,

Sehon³

1977

Int Arch Allergy Immunol

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The low molecular weight dialyzable fraction (D) prepared from the aqueous extract of Kentucky Blue Grass pollen was shown to suppress the formation of IgE antibodies in rats immunized with the nondialyzable fraction (R). In an attempt to establish the nature of the constituents responsible for this suppression, D was fractionated by gel filtration through Sephadex G-25. The first fraction eluted (D_I) elicited skin reactions in rats sensitized with a rat reaginic serum to R and also gave a precipitate with a rabbit antiserum to R. A later fraction (D_III) was devoid of these two properties. To investigate the effects of these fractions on the antibody response, rats received either D_I or D_III, administered in saline, prior to their immunization with R in presence of aluminum hydroxide. Pretreatment with D_I resulted in a reduction of IgE antibody levels as compared with the IgE antibody response in control animals which had received pretreatment only with saline; however, pretreatment with D_I did not affect the anti-R-hemagglutinating titers. In contrast, pretreatment with D_III enhanced both the IgE and the hemagglutinating antibodies. Hence, it is concluded that the composite fraction D contains one group of constituents capable of suppressing and another of enhancing the IgE antibody response.

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“…In support of this interpretation, it is to be noted that excessively large amounts of the haptenic preparation were re quired, in relation to the amounts of the non-dialyzable aqueous extract of ragweed pollen, for the complete neutralization of reaginic activity of ragweed allergic sera [1]. Moreover, it ought to be pointed out that the haptenic preparation was composed of at least four components possess ing distinct electrophoretic mobilities and it still remains to be determined which of these components is (are) the hapten(s) capable of combining with reagins.…”

Section: Discussionmentioning

confidence: 99%

“…The haptenic preparation, which was not skin-active, had been shown to be capable of neutralizing completely and specifically the reaginic ac tivity of ragweed allergic sera [1], As a plausible explanation for the rela tively low recovery of reagins by elution with haptenic components one may suggest that only reagins with relatively low affinities for the aller genic determinants of R-Rag were displaced by these ragweed constituent haptens. The possibility that such low affinity reagins were displaced by salt effects due to the low molecular weight constituents can be ruled out in view of the inability of this ragweed haptenic preparation to displace reagins to allergenic constituents of Timothy grass from the appropriate immunosorbent.…”

Section: Discussionmentioning

confidence: 99%

“…In an attempt to recover reagins by specific displacement from the immunosorbent with a dialyzable ragweed prepa ration [1] possessing hapten-like properties (referred to hereafter as hapten), 40 mg of immunosorbent-antibody complex was suspended in a volume of 2 ml of a solu tion containing 200 mg of hapten and 10 mg of HSA. After stirring for 30 min at room temperature the immunosorbent was removed by centrifugation, the superna tant recovered and dialyzed extensively against the Nal buffer to dissociate the antibody-hapten complexes and to facilitate the removal of the haptens, then against the tris-HCl buffer alone and finally against PBS.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Isolation of Human Reagins Differing in Their Affinity for Ragweed Immunosorbent

Kisil¹,

Attallah²,

Sehon³

et al. 1974

Int Arch Allergy Immunol

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Reaginic antibodies to ragweed pollen which had been adsorbed to cellulose-allergen immunosorbents were eluted in the presence of homologous or heterologous serum protein(s) at a concentration of 3–7 mg/ml with gly-HCl at pH 2.5, or 6 m urea at pH 7.4. These extraneous serum proteins exerted a stabilizing effect on the eluted reagins. Two groups of reagins differing in their affinity for the allergen(s) were sequentially eluted from the immunosorbent with gly-HCl and Nal regardless of the order of addition of these eluting agents. Moreover, specific elution of some reagins was achieved with a low molecular weight, hapten-like preparation of ragweed pollen.

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Isolation of haptenic material from ragweed pollen

Cited by 23 publications

References 9 publications

Developments in allergen‐specific immunotherapy: from allergen extracts to allergy vaccines bypassing allergen‐specific immunoglobulin E and T cell reactivity

Developments in allergen‐specific immunotherapy: from allergen extracts to allergy vaccines bypassing allergen‐specific immunoglobulin E and T cell reactivity

Modulation of the IgE Antibody Response in Rats to Kentucky Blue Grass Pollen Allergens

Isolation of Human Reagins Differing in Their Affinity for Ragweed Immunosorbent

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