Isolation of glycopeptides with skin test activity from dermatophytes

Abstract

By using ethylene glycol extraction of whole submerged cultures, followed by Sephadex G-200 and diethylaminoethyl-Sephadex chromatography, we isolated four distinct glycopeptides from Trichophyton mentagrophytes, T. rubrum, and Microsporum canis. Chemical analyses revealed that these glycopeptides contained mostly carbohydrate (42.5 to 81.6%) and protein (4.3 to 11.3%), with lesser amounts of phosphorus (0.4 to 6.0%) and hexosamines (0.3 to 0.6%). Based upon total carbohydrate and monosaccharide content, these dermatophyte glycopeptides could be divided into two chemical groups: glucopeptides (DSI) and mannopeptides (DSI2, DS11, and DS112). The mannopeptides and glucopeptides of each species of dermatophyte were not significantly different chemically from those derived from the other two dermatophyte species studied. Skin testing of DSI1-glucopeptides or DSI2-mannopeptides in immunized guinea pigs indicated that only the DSI2-mannopeptides elicited a delayed hypersensitivity reaction. Skin testing T. mentagrophytes 62-infected guinea pigs with the four purified DSglycopeptides, as well as earlier fractions from the purification scheme, derived from T. mentagrophytes, T. rubrum, and M. canis, again indicated that only the DSI2-mannopeptides of the two Trichophyton species elicited a delayed hypersensitivity reaction. The number of infections or duration of infection had no effect on the size of the skin test response. DSI2-mannopeptides were non-crossreactive between genera when tested in Trichophyton-immunized or-infected guinea pigs and Microsporum-immunized guinea pigs.

Human dermatophyte‐responsive T‐cell lines recognize cross‐reactive antigens associated with mannose‐rich glycoproteins

“…Evidence suggests that a carbohydrate/protein complex mediates both humoral and cell-mediated immunity (10,26). Moser and Pollack have isolated a series of glycopeptides from dermatophytes which were capable of inducing delayed hypersensitivity in guinea pigs (26). Both cross-reactive and species-specific antigen fractions were identified.…”

“…Portiotis of this work were reported at the Society for Investigative Dertnatology Annual Meeting in Washington D.C. on April [26][27][28][29][30]1989. An abstract of this presentation appears in (Tlin Res 37: 652A, 1989. quinekeanum in sublethally irradiated BALB/c mice as measured by the size of the fungal lesion (32).…”

Cutaneous defenses against dermatophytes and yeasts

“…The dermatophytes, like other fungi, have a very complicated antigenic makeup. Antigenic substances of the dermatophytes may be glycopeptides, peptides, or carbohydrates, and the individual types of antigens may elicit different types of responses (14,184). In addition, the keratinase of Trichophyton mentagrophytes may be an important antigen not only because it elicits strong delayed hypersensitivity responses but also because antibodies that inhibit the proteolytic activity of this enzyme are produced (97).…”

Απομονωση Τυποποιηση Δερματοφυτων. Μελετη Συνθηκων Της Σπορογονιας Των

“…Παρ'όλα αυτά οι ερευνητές αυτοί δεν μελέτησαν ποικιλία συγκεντρώσεων θειαμίνης και περιορίσθηκαν σε συγκεντρώσεις μεγαλύτερες του 0,6g/l. (Shadomy and Philpot 1980, Kumert 1988, Clayton and Midgley 1989 Moser and Pollack (1978) οι οποίες όμως συν δέθηκαν με την περιεκτικότητα του κυτταρικού τοιχώματος αυτών των μυκήτων σε γλυκοπρωτεϊνες και όχι με τις τροφι κές απαιτήσεις τους in vitro.…”