Isolation of Glycoalkaloids with the Chromatotron and Their Determination by High-Performance Liquid Chromatography

Abstract

An e x t r a c t of Solanum l a c i n i a t u m leaves was f r a c t i o n a t e d w i t h t h e Chromatotron. The m k a l o i d f r a c t i o n t h u s o b t a i n e d was analyzed by reversed-phase HPLC. The l o a d c a p a c i t y o f t h e Chromatotron w i t h a 2-mm l a y e r t h i c k n e s s i s 600 mg (as determined w i t h c h o l e s t e r o l ) and t h e recovery o f solasonine from t h e p l a n t e x t r a c t (as determined by HPLC) i s 93-96%. The HPLC method p e r m i t s t h e d e t e c t i o n o f as l i t t l e as 1.5 ug solasonine o r 3.5 p g solamargine w i t h a l i n e a r d e t e c t o r response up t o 375 pg f o r t h e former and 250 u g f o r t h e l a t t e r .

Solanum dulcamara L. (Bittersweet): Accumulation of Steroidal Alkaloids in the Plant and in Different in Vitro Systems

“…The crude glycoalkaloids can be precipitated by the addition of NH4 0H; extraction of the supernatant with butanol yields the saponin fraction. The direct identification of glycoalkaloids (e.g., solasonine, solamargine) is possible by HPLC (Elridge and Hockridge 1983;Hunter and Heftmann 1983; in combination with fast atom bombardment (FAB) or thermospray mass spectrometry (Price et al 1985;Ehmke et al 1987). For the sensitive and specific determination of solasodine glycosides in minute samples, Weiler et al (1980) established a radioimmunoassay.…”

Preparation and Application of Reversed Phase ChromatorotorTM for the Isolation of Natural Products by Centrifugal Preparative Chromatography

“…Centrifugal preparative chromatography (CPC) is a convenient, reliable and economic method for preparative scale separation of natural products [1][2][3][4]. Devices for CPC have long been recorded in the art of separation.…”