Isolation of Genomic Fragments from Polymorphic Regions by Representational Difference Analysis

Baldocchi, Russell A.; Flaherty, Lorraine

doi:10.1006/meth.1997.0542

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Cited by 5 publications

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“…16 second received 150 ng of lambda DNA (pool B: 1000 copies of lambda/human genome), and the third received no lambda DNA (pool C). Two rounds of RDA were performed in an attempt specifically to amplify a 570 bp lambda HindIII fragment.…”

Section: Resultsmentioning

confidence: 99%

Appraisal of genetic and epigenetic congruity of a monozygotic twin pair discordant for schizophrenia

McDonald

2003

Journal of Medical Genetics

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Section: Resultsmentioning

confidence: 99%

Appraisal of genetic and epigenetic congruity of a monozygotic twin pair discordant for schizophrenia

McDonald

2003

Journal of Medical Genetics

View full text Add to dashboard Cite

Genome Scanning

Plass

2002

Wiley Encyclopedia of Molecular Medicine

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Resistance gene analogue markers are mapped to homeologous chromosomes in cultivated tetraploid cotton

Hinchliffe

Potenza

et al. 2005

Theor Appl Genet

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Degenerate primers designed from conserved motifs of known plant resistance gene products were used to amplify genomic DNA sequences from the root-knot nematode (Meloidogyne incognita) resistance genetic source, Upland cotton (Gossypium hirsutum) cultivar Auburn 634 RNR. A total of 165 clones were isolated, and sequence analysis revealed 57 of the clones to be novel nucleotide sequences, many containing the resistance (R)-protein nucleotide-binding site motif. A cluster analysis was performed with resistance gene analogue (RGA) nucleotide sequences isolated in this study, in addition to 99 cotton RGA nucleotide sequences already deposited in GenBank, to generate a phylogenetic tree of cotton R genes. The cotton RGA nucleotide sequences were arranged into 11 groups and 56 sub-groups, based on genetic distances. Multiple sequence alignments were performed on the RGA sequences of each sub-group, and either the consensus sequences or individual RGA sequences were used to design 61 RGA-sequence-tagged site primers. A recombinant inbred line (RIL) population of cultivated tetraploid cotton was genotyped using RGA-specific primers that amplified polymorphic fragments between the two RIL parents. Nine RGA markers were mapped to homeologous chromosomes 12 and 26, based on linkage to existing markers that are located on these chromosomes.

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Isolation of Genomic Fragments from Polymorphic Regions by Representational Difference Analysis

Cited by 5 publications

References 21 publications

Appraisal of genetic and epigenetic congruity of a monozygotic twin pair discordant for schizophrenia

Appraisal of genetic and epigenetic congruity of a monozygotic twin pair discordant for schizophrenia

Genome Scanning

Resistance gene analogue markers are mapped to homeologous chromosomes in cultivated tetraploid cotton

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