Isolation of genomic DNA using magnetic cobalt ferrite and silica particles

Abstract

Adsorption separation techniques as an alternative to laborious traditional methods (e.g., based on phenol extraction procedure) have been applied for DNA purification. In this work we used two types of particles: silica and cobalt ferrite (unmodified or modified with a reagent containing weakly basic aminoethyl groups, aminophenyl groups, or alginic acid). DNA from chicken erythrocytes and DNA isolated from bacteria Lactococcus lactis were used for testing of adsorption/desorption properties of particles. The cobalt ferrite particles modified with different reagents were used for isolation of PCR-ready bacterial DNA from different dairy products.

DNA extraction from plant food supplements: Influence of different pharmaceutical excipients

“…These results are in good agreement with previous data that reported a very strong interaction between DNA and particles of cobalt ferrite (a compound composed of iron oxide) due to the high affinity of phosphate groups to iron oxides [22].…”

Synthesis of Spinel CoFe2O4 Via the Co‐Precipitation Method Using Tetraalkyl Ammonium Hydroxides as Precipitating Agents

“…C obalt ferrite belongs to a family of AFe 2 O 4 ‐type spinel ferrites 1–3 (where A is a divalent metal). Ferrites have diverse practical applications as magnetic materials, 4–6 refractory materials, 7 in medical diagnostics, 8,9 and as catalysts 10 . Because their properties are critically dependent on the synthesis procedure, it is of interest to explore the cause and effect relationship between synthetic strategies and the properties of these materials.…”

Resistive pulse sensing of magnetic beads and supraparticle structures using tunable pores

“…40 They are of particular benefit in assay development and biological handling applications, providing an efficient and cost-effective way to separate and pre-concentrate analytes from solutions. [41][42][43][44] Their versatility has been demonstrated by use in conjunction with techniques such as the polymerase chain reaction, 45 mass spectroscopy, 46 and high-throughput linear magnetophoresis (LM) assays. 41,[47][48][49][50] Precise application of magnetic fields has enabled device-based quadrupole sorting, [51][52][53][54] active bead transport for biosensing, 55 and dynamic manipulation of magnetic supraparticle structures (SPSs).…”