Isolation of genomic DNA from buccal swabs for forensic analysis, using fully automated silica-membrane purification technology

Hanselle, Thomas; Otte, M.; Schnibbe, Thomas; Smythe, Emma; Krieg-Schneider, F.

doi:10.1016/s1344-6223(02)00099-8

Cited by 17 publications

(9 citation statements)

References 9 publications

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“…The use of buccal swabs is becoming a common method for collecting human and nonhuman DNA samples because it is rapid, non-invasive, and has been shown to produce a sufficient yield for a variety of genetic assays [[1], [2], [3]]. However, there remains a dearth of independent investigation into the DNA yield acquired from various medical and non-medical swab types despite the stark cost difference between the two.…”

Section: Methods Detailsmentioning

confidence: 99%

Medical-grade buccal swabs versus drugstore cotton swabs: No difference in DNA yield

2018

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Section: Methods Detailsmentioning

confidence: 99%

Medical-grade buccal swabs versus drugstore cotton swabs: No difference in DNA yield

2018

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“…However, the current available methods are cumbersome and time-consuming. 1,2 Commercially available methods use silica columns to capture DNA in the presence of a high salt solution from human samples. 1,3 Protein molecules and other debris are then washed with alcohols and buffers.…”

Section: Introductionmentioning

confidence: 99%

Nanotips for single-step preparation of DNA for qPCR analysis

Kalyanasundaram

Kim

Fotouhi

et al. 2013

Analyst

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PCR inhibitory substances in complex sample matrices can cause false negatives or underestimation of target concentration. This study assessed DNA heat treatment for reducing inhibition during qPCR analysis of human adenovirus (HAdV) in wastewater samples. Inhibition was reduced by heat treating DNA, where mean HAdV concentration was increased by 0.71 log 10 GC/L (and up to 3.04 log 10 GC/L in one case), and replicate variability and false negatives were reduced. DNA heat treatment should be further investigated for improving reliability of HAdV concentration estimates in water, which can support more accurate assessment of health risks associated with viral pathogen exposure.

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“…DNA extraction is also crucial for medical, forensic, environmental, or military purposes [1]. Popular sources are saliva- [2, 3] and buccal swab samples [4, 5] because the sample collection is minimally invasive.…”

Section: Introductionmentioning

confidence: 99%

“…For DNA extraction, solid phase extraction (SPE) methods using porous silica are commercially available[5, 6]. Cell lysates are infiltrated into silica micropores by high salt and chaotropic solutions, which bind DNA by electrostatic charge.…”

Section: Introductionmentioning

confidence: 99%

Rapid extraction and preservation of genomic DNA from human samples

et al. 2013

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Simple and rapid extraction of human genomic DNA remains a bottle neck for genome analysis and disease diagnosis. Current methods using microfilters require cumbersome, multiple handling steps in part because salt conditions must be controlled for attraction and elution of DNA in porous silica. We report a novel extraction method of human genomic DNA from buccal swab- and saliva samples. DNA is attracted on to a gold-coated microchip by an electric field and capillary action while the captured DNA is eluted by thermal heating at 70 °C. A prototype device was designed to handle 4 microchips, and a compatible protocol was developed. The extracted DNA using microchips was characterized by qPCR for different sample volumes, using different lengths of PCR amplicon, and nuclear and mitochondrial genes. In comparison with a commercial kit, an equivalent yield of DNA extraction was achieved with fewer steps. Room-temperature preservation for one month was demonstrated for captured DNA, facilitating straightforward collection, delivery and handling of genomic DNA in an environment-friendly protocol.

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Isolation of genomic DNA from buccal swabs for forensic analysis, using fully automated silica-membrane purification technology

Cited by 17 publications

References 9 publications

Medical-grade buccal swabs versus drugstore cotton swabs: No difference in DNA yield

Medical-grade buccal swabs versus drugstore cotton swabs: No difference in DNA yield

Nanotips for single-step preparation of DNA for qPCR analysis

Rapid extraction and preservation of genomic DNA from human samples

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