Isolation of genes encoding novel transcription factors which interact with the Hap complex from Aspergillus species

Tanaka, Akimitsu; Kato, Masashi; Nagase, Takeshi; Kobayashi, Tetsuo; Tsukagoshi, Norihiro

doi:10.1016/s0167-4781(02)00286-5

Cited by 35 publications

(31 citation statements)

References 34 publications

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“…The Gly residue, where the missense mutation FlbB100 (G70R) lies, is situated nine residues upstream from the starting Leu of the bZIP domain. Previous reports have shown the presence a similar sequence in HapX, where this Gly is universally conserved (41). This region is necessary for the recruitment of HapX to the Hap complex and consequent recognition of CCAAT sequence-containing promoter regions (25).…”

Section: Discussionmentioning

confidence: 87%

Basic-Zipper-Type Transcription Factor FlbB Controls Asexual Development in Aspergillus nidulans

et al. 2008

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The fungal colony is a complex multicellular unit consisting of various cell types and functions. Asexual spore formation (conidiation) is integrated through sensory and regulatory elements into the general morphogenetic plan, in which the activation of the transcription factor BrlA is the first determining step. A number of early regulatory elements acting upstream of BrlA (fluG and flbA-E) have been identified, but their functional relations remain to be further investigated. In this report we describe FlbB as a putative basiczipper-type transcription factor restricted to filamentous fungi. FlbB accumulates at the hyphal apex during early vegetative growth but is later found in apical nuclei, suggesting that an activating modification triggers nuclear import. Moreover, proper temporal and quantitative expression of FlbB is a prerequisite for brlA transcription, and misscheduled overexpression inhibits conidiation. We also present evidence that FlbB activation results in the production of a second diffusible signal, acting downstream from the FluG factor, to induce conidiation.

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Section: Discussionmentioning

confidence: 87%

Basic-Zipper-Type Transcription Factor FlbB Controls Asexual Development in Aspergillus nidulans

et al. 2008

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“…Among these, only one factor, HapX, has been reported. 93) In A. nidulans, METR is the only b-ZIP factor so far characterized. METR is a transcriptional activator in sulfur metabolism.…”

Section: Proteolytic Enzymesmentioning

confidence: 99%

Genomics ofAspergillus oryzae

Kobayashi

Abe

Asai

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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118

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“…Originally, a yeast two-hybrid screen using HapB as bait identified HapX as an additional subunit of the CBC in A. nidulans (15). HapX displays no similarity to S. cerevisiae Hap4p, except for an N-terminal 17-amino acid motif, which has been shown to be essential for interaction of Hap4p with the S. cerevisiae Hap2p-Hap3p-Hap5p complex (16).…”

mentioning

confidence: 99%

Deciphering the Combinatorial DNA-binding Code of the CCAAT-binding Complex and the Iron-regulatory Basic Region Leucine Zipper (bZIP) Transcription Factor HapX

Hortschansky

Ando

Tuppatsch

et al. 2015

Journal of Biological Chemistry

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Background: HapX and the CCAAT-binding complex (CBC) are the master regulators of fungal iron homeostasis. Results: HapX DNA binding requires previous CBC-DNA complex formation and a 3Ј-flanking GAT motif with variable nucleotide spacing. Conclusion: Combinatorial CBC-HapX DNA recognition allows discrimination of CBC and CBC-HapX targets. Significance: Conservation of functional domains in HapX orthologs suggests a similar DNA-binding code in most fungi.

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Isolation of genes encoding novel transcription factors which interact with the Hap complex from Aspergillus species

Cited by 35 publications

References 34 publications

Basic-Zipper-Type Transcription Factor FlbB Controls Asexual Development in Aspergillus nidulans

Basic-Zipper-Type Transcription Factor FlbB Controls Asexual Development in Aspergillus nidulans

Genomics ofAspergillus oryzae

Deciphering the Combinatorial DNA-binding Code of the CCAAT-binding Complex and the Iron-regulatory Basic Region Leucine Zipper (bZIP) Transcription Factor HapX

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