Isolation of Ganoderma lucidum (Curtis) P. Karst. From the Wild in Lagos through Tissue Culture Techniques and Cultivation on Sawdust of Six Nigerian Hardwoods

Abstract


 
 
 Cultivation of Ganoderma lucidum (Curtis) P. Karst, a medicinal mushroom known for antioxidant, antitumor, anti-inflammatory, and immunomodulatory activities is not practiced in Nigeria. Tree species used for cultivation in Asia, America and Europe are not available in Nigeria. The present study investigated indigenous hardwoods and their supplementation with rice and wheat bran as substrates for its cultivation. Six hardwoods (Mansonia altissima (A Chev.) A Chev., Avecennia germinans (L.) L, Lophira alata Banks ex Gaertn., Triplochiton scleoxylon K. Schum, Uapaca guineensis Mull. Arg, Nauclea diderrichii (De Wild. & Th. Dur.) Merrill) were investigated as potential growth substrates and potentially improve biological efficiency. The mushroom was collected from the wild and identification confirmed by amplifying the ribosomal DNA-ITS fragment with ITS1 and ITS4 primers. Tissue culture of the mushroom collected from the wild was initiated successfully with modified malt extract agar and grain spawn developed from it. The substrates were incubated after inoculation with grain spawn at room temperature for 30-60 days. Substrates were given cold treatment for 7days to induce fructification which was achieved with 9-10h/day regime and daily watering. There was full mycelial ramification of all substrates by the mushroom. Fruit bodies were harvested from all substrates but Lophira alata. The highest yield (308.76±5.81g/kg) was recorded with Mansonia altissima (Biological Efficiency-31.42±4.55%) and the least yield (31.45±5.44g/kg) was recorded in Nauclea diderrichii (B.E- 5.25±0.58%). The substrates with wheat bran performed significantly better than rice bran supplemented substrates. Commercial cultivation of native G. lucidum in Nigeria is possible with local agricultural wastes.
 
 

Ganoderma lucidum: Unutilized natural medicine and promising future solution to emerging diseases in Africa

“…Diverse substrate formulations have been reported and employed for the large-scale production of this mushroom to meet its demand. In Lagos Nigeria, Adongbede and Atoyebi, (2021) cultivated G. lucidum (Curtis) P. Karst. on the sawdust (substrate) of six indigenous hardwoods supplemented with rice and wheat bran.…”

Effects of cultivation substrate composition on biological productivity and quality parameters of Ganoderma lucidum

“…During the process of cultivating reishi mushrooms, several researchers have added numerous additives (such as wheat and corn and rice bran, soybean meal, cottonseed meal, malt, sugarcane bagasse, sunflower meal or molasses) to corncobs and straw (Veena and Pandey, 2011;Rashad et al, 2019;Yuliana et al, 2020), sawdust of oak, mango, acacia, tuni, paddy straw, wheat straw, and soybean waste (Jandaik et al, 2013), sawdusts of sheesham, mango, and poplar (Mehta et al, 2014), sunflower seed husks (Gonzalez-Matute et al, 2002), paddy husk and plant waste (Yang et al, 2003;Rashad et al, 2019;Yuliana et al, 2020), tea waste (Peksen and Yakupoğlu, 2009), sawdust of various trees (Erkel, 2009;Nguyen et al, 2019;Atila, 2020;Adongbede and Atoyebi, 2021), olive plant residues (Koutrotsios et al, 2019), broad bean stalks, cotton stalks and wheat straw (Rashad et al, 2019;Atila, 2020), soybean straw and bean straw (Atila, 2020), hazelnut shells (Puliga et al, 2022) and found differences among the substrates. Growers generally prefer to use the best and cheapest substrate materials available locally (Özçelik and Pekşen, 2007).…”