Isolation of Gangliosides.

Svennerholm, Lars; Lode, Aaslaug; Sömme, R.; Andersen, Inger Grete Krogh; Munch‐Petersen, Jon

doi:10.3891/acta.chem.scand.17-0239

Cited by 50 publications

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“…Horse erythrocyte glycopeptides were isolated by gel filtration after three pronase digestions [ 12] of the membrane fractions. Crude extracts of gangliosides were obtained by Svennerholm's method [13], as modified by Weinstein et al [14] from membrane fraction (HEGN) or from the insoluble pellet after pronase digestion of membrane fractions (HEGP).…”

Section: Methodsmentioning

confidence: 99%

Interaction between vesicles containing gangliosides and lectins Limulin and wheat‐germ agglutinin

1977

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Section: Methodsmentioning

confidence: 99%

Interaction between vesicles containing gangliosides and lectins Limulin and wheat‐germ agglutinin

1977

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“…Lactosylceramide was isolated from human spleen by TLC (19) or prepared from human brain gangliosides by mild acid hydrolysis (0.1 M HCI at 100" for 1 h). Ganglioside G,, was isolated by column chromatography of crude brain gangliosides (20) treated by sialidase at pH 7.2 and saponified. The purified G,, was then isolated by renewed chromatography on silicic acid.…”

Section: Substratesmentioning

confidence: 99%

Chemical Pathology of Krabbe's Disease

1975

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Galactosylceramide beta-galactosidase and lactosylceramide beta-galactosidase activities were investigated in normal human brain, leu-kocytes and amniotic fluid cells. The enzymatic assays were performed on brains from 11 patients with Krabbe's disease, on leukocytes from 16 patients and 18 obligate heterozygotes, and on amniotic fluid cells from 9 foetuses at risk. The brain enzyme was solubilized from a 900 g-100000 g pellet. With this enzyme preparation a profound deficiency of galactosylceramide beta-galactosidase activity in brain, approximately 1% of that in age-matched controls was shown. The lactosylceramide beta-galactosidase activity of brain was also strongly reduced, but not to the same extent as the other beta-galactosidase. Galactosylceramide beta-galactosidase activity in leukocytes from patients with Krabbe's disease was generally less than 5% of that in age-matched controls and there was no overlap between the patients and the obligate heterozygotes. Carrier detection by the leukocyte enzyme was, however, not possible because of considerable overlap between heterozygotes and normal controls. The lactosylceramide beta-galactosidase activity was only moderately reduced in leukocytes, but strongly reduced in cerebral tissue from patients with Krabbe's disease. The changes in the glycolipid pattern of cerebral tissue, recently described by us in patients with Krabbe's disease, offers an explanation to the serious glycolipid beta-galactosidase deficiency in CNS.

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“…These results support the use of cerebrosides as a biochemical index to depict the occurrence of myelin in normal and pathological nervous tissue. The water-insoluble glycolipids of the nervous system consist almost entirely of galactocerebrosides; glucocerebrosides and ceramide-oligosaccharides are found in only trace amounts, although they are more abundant in other tissues (SVENNERHOLM and SVENNERHOLM, 1963; CARTER, JOHN-SON and WEBER, 1965). Furthermore, galactocerebrosides are largely confined to the…”

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A COMPARISON OF CEREBROSIDES, PROTEOLIPID PROTEINS, AND CHOLESTEROL AS INDICES OF MYELIN IN THE ARCHITECTURE OF RAT CEREBRUM¹

Bass

Hess

1969

Journal of Neurochemistry

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Abstract— —Serial frozen section sampling and microspectrophotometric assays were used to compare the fidelity of cerebrosides, proteolipid proteins (PLP) and cholesterol as indices of myelinated fibres in the somatosensory region of rat cerebrum. Cerebrosides (including sulphatides) were a satisfactory index regardless of the mode of expression. Per unit dry wt., they were lowest in layer II (4 per cent) and increased with the subpial depth to 16 percent in white matter. Elevations were seen at the locations of: the outer tangential plexus (1); the stripe of Kaes‐Bechterew (2); the outer and inner stripes of Baillarger (3,4); and the inner tangential plexus (5), respectively. Expression in terms of residue protein, total lipid or DNA gave somewhat sharper profiles of the myeloarchitecture. PLP per unit dry wt. were lowest in layers I, II and III (2 per cent) and increased to 5·5 per cent in white matter. Peaks occurred near (3), (4) and (5). The PLP per cell and per unit residue protein rose six‐fold, from the upper layers of cortex to white matter, and depicted the myeloarchitecture more faithfully. In general outline, PLP paralleled cerebrosides in distribution, in contrast with previous findings in human prefrontal cortex. Human cortex may contain more non‐myelin PLP in the upper cortical layers owing to its greater wealth of neuropil and synapses. Cholesterol per unit dry weight was a poor index of the myeloarchitecture; it was lowest in layer II (5 per cent) and increased to 13‐5 per cent in white matter without revealing the five horizontal bands of myelinated fibres. Expression of cholesterol in terms of total lipid or DNA improved its value as an index, but it was less satisfactory than cerebrosides.

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Isolation of Gangliosides.

Cited by 50 publications

References 0 publications

Interaction between vesicles containing gangliosides and lectins Limulin and wheat‐germ agglutinin

Interaction between vesicles containing gangliosides and lectins Limulin and wheat‐germ agglutinin

Chemical Pathology of Krabbe's Disease

A COMPARISON OF CEREBROSIDES, PROTEOLIPID PROTEINS, AND CHOLESTEROL AS INDICES OF MYELIN IN THE ARCHITECTURE OF RAT CEREBRUM¹

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Isolation of Gangliosides.

Cited by 50 publications

References 0 publications

Interaction between vesicles containing gangliosides and lectins Limulin and wheat‐germ agglutinin

Interaction between vesicles containing gangliosides and lectins Limulin and wheat‐germ agglutinin

Chemical Pathology of Krabbe's Disease

A COMPARISON OF CEREBROSIDES, PROTEOLIPID PROTEINS, AND CHOLESTEROL AS INDICES OF MYELIN IN THE ARCHITECTURE OF RAT CEREBRUM1

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A COMPARISON OF CEREBROSIDES, PROTEOLIPID PROTEINS, AND CHOLESTEROL AS INDICES OF MYELIN IN THE ARCHITECTURE OF RAT CEREBRUM¹