Isolation of follicular dendritic cells from human tonsils and adenoids. I. Procedure and morphological characterization

Lilet-Leclercq, Chantal; Radoux, D.; Heinen, Ernst; Kinet-Denoel, C.; Defraigne, Jean-Olivier; Houben-Defresne, Marie-Paule; Simar, Léopold

doi:10.1016/0022-1759(84)90334-x

Cited by 61 publications

(11 citation statements)

References 14 publications

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“…FDCs and lymphoid dendritic cells (interdigitating reticulum cells) are morphologically similar, low density cells. Both cell types provide in vivo and in vitro a complex microenvironment for lymphoid cells by forming extensive networks with cellular processes (29)(30)(31). This report, however, demonstrated that FDCs could be identified as a unique cell type with phenotypic characteristics that were distinct from those of lymphoid dendritic cells.…”

Section: Discussionmentioning

confidence: 75%

Isolated human follicular dendritic cells display a unique antigenic phenotype.

Schriever

Freedman

Freeman

et al. 1989

The Journal of Experimental Medicine

204

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In the present study, follicular dendritic cells (FDCs) were purified to homogeneity in order to define the lineage and function of these cells. FDCs were identified by their characteristic morphology and by their expression of receptors for the third complement component, the myeloid-restricted antigen CD14, and the FDC antigen DRC-1. Unclustered FDCs displayed a unique antigenic phenotype since they expressed several B- and myeloid lineage-restricted antigens, but lacked T and NK cell antigens as well as the leukocyte common antigen. FDCs expressed adhesion molecules, including most of the VLA proteins, intercellular adhesion molecule 1 (ICAM-1), and CD11b. FDCs could be isolated to homogeneity by their intense staining with anti-CD14 using flow cytometric cell sorting. These highly purified FDCs expressed CD14 and CD21 but lacked CD20. This antigen pattern and characteristic morphology confirmed that these cells were, in fact, homogeneous FDC preparations. Analysis of polymerase chain reaction-amplified cDNA from highly purified FDCs showed no transcripts for IL-6. The isolation of homogeneous FDC populations will be important for the analysis of the functional role of FDCs within the lymphoid follicle.

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Section: Discussionmentioning

confidence: 75%

Isolated human follicular dendritic cells display a unique antigenic phenotype.

Schriever

Freedman

Freeman

et al. 1989

The Journal of Experimental Medicine

204

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“…However, thusfar, preparation of FDC from normal lymphoid tissue yielded in cell suspensions containing only 10% to 50% FDC clusters with the FDC still being aggregated with each other and/or with lymphoid cells [5, [11][12][13][14].Thus, the aim of our study was to develop a technique for the preparation of highly pure single-cell suspensions of viable FDC and to elucidate whether these cells might be susceptible to HIV-1 infection in vitro.…”

Section: Introductionmentioning

confidence: 99%

Isolation of normal human follicular dendritic cells and CD4‐independent in vitro infection by human immunodeficiency virus (HIV‐1)

et al. 1991

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Immunohistological and electron microscopy studies of lymph nodes from patients infected with the human immunodeficiency virus 1 (HIV-1) demonstrated that follicular dendritic cells (FDC), the antigen-presenting cells of the B cell system, contain and may produce the virus. To elucidate the mode of infection of FDC with HIV-1 in vitro we developed an improved method for the preparation of single-cell suspensions of viable FDC with high purity (greater than 90% FDC). These isolated FDC were subjected to human T cell leukemia virus IIIB infection, which was monitored after 4 days in culture using the polymerase chain reaction. We were able to demonstrate that normal human FDC are highly susceptible to infection by HIV-1. Inhibition experiments with the monoclonal antibody OKT4a demonstrate that this infection is independent of the CD4 molecule.

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“…Antibodies directed against the T3 antigen block cell-mediated lysis and stimulate resting T cells to proliferate [5]. The murine equivalent of the human T3 has not been defined unequivocally as yet [6, 71.…”

Section: Introductionmentioning

confidence: 99%

Transfer of immune complexes from lymphocytes to follicular dendritic cells

et al. 1986

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Antigens in the form of immune complexes are retained on the membranes of follicular dendritic cells (FDC) for long periods of time. To examine how immune complexes reach germinal centers, where FDC are located, we injected mice with anti-2,4-dinitrophenyl (DNP) antibodies complexed to DNP-myoglobin-coated gold particles. The distribution of the particles in spleens or draining lymph nodes was then determined with the electron microscope. The vast majority of the particles were cell bound. Shortly after injection they were phagocytized by macrophages or fixed on lymphocytes. The latter were found even in the corona of lymph follicles but not in germinal centers. Already 30 min after injection, FDC in contact with the corona were faintly positive but were negative in the center. FDC precursor cells were occasionally observed but in too small a number to account for the transport of immune complexes to the germinal centers. Twenty-four hours after injection colloidal gold particles were found in phagolysosomes of macrophages or on cytoplasmic extensions of FDC in all parts of the germinal centers. Experiments performed on isolated FDC showed that they are not only able to take up free immune complexes but are also able to adsorb immune complexes from pulsed lymphocytes. These results strengthen the idea that lymphoid cells binding immune complexes by their Fc receptors may transport these complexes inside germinal centers.

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Isolation of follicular dendritic cells from human tonsils and adenoids. I. Procedure and morphological characterization

Cited by 61 publications

References 14 publications

Isolated human follicular dendritic cells display a unique antigenic phenotype.

Isolated human follicular dendritic cells display a unique antigenic phenotype.

Isolation of normal human follicular dendritic cells and CD4‐independent in vitro infection by human immunodeficiency virus (HIV‐1)

Transfer of immune complexes from lymphocytes to follicular dendritic cells

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