Isolation of Filamentous Fungi from the Caatinga Region and Production of Amylolytic Enzymes of Great Industrial Interest

Amorim, Ingrid Cristina Soares; Marinho, Gessica Oliveira; Oliveira, Tarcisio Michael Ferreira Soares De; Roa, Jorge; Reis, Arlete Barbosa dos; Nelson, David Lee; Pasin, Thiago Machado; Benassi, Vívian Machado

doi:10.4236/jbm.2020.811014

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…Experiments were carried out with A. japonicus , previously isolated from Atlantic Forest remnants 17 and deposited as URM 7270 at the mycology collection URM (WDCM 604)/ISO 9001:2015 from the Department of Mycology at the Federal University of Pernambuco, Recife, Pernambuco, Brazil. A. japonicus was maintained at the Laboratory of Microbiology and Cell Biology from the Biology Department of the Faculty of Philosophy, Sciences, and Letters of Ribeirão Preto, University of São Paulo, Brazil, in slants of solid culture medium composed of 4% oatmeal (w/v) and 2% bacteriological agar (w/v) 18 . Samples of the fungus were kept at −80°C in a solution of 20% glycerol (v/v) or at 4°C in silica, as previously reported by Pasin et al 17 The culture of A. japonicus was scraped and the spores were resuspended in sterile distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…Chemicals: Potato starch from Sigma-Aldrich ® -Brazil, oatmeal from Quaker ® -Brazil, bacteriological agar from Sigma-Aldrich ® -Brazil, starch from Reagen ® -Brazil, 3,5-Dinitrosalicylic acid from Sigma-Aldrich ® -Brazil, D-glucose from Sigma-Aldrich ® -Brazil, sodium acetate from Sigma-Aldrich ® -Brazil, sodium phosphate from Sigma-Aldrich ® -Brazil, sodium bicarbonate from Sigma-Aldrich ® -Bra- 18 Samples of the fungus were kept at À80 C in a solution of 20% glycerol (v/v) or at 4 C in silica, as previously reported by Pasin et al 17 The culture of A. japonicus was scraped and the spores were resuspended in sterile distilled water. Spores were counted in a Neubauer chamber, and a solution containing 1.77 Â 10 6 spores/mL was obtained.…”

Section: Chemical and Instrumentsmentioning

confidence: 99%

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Biochemical characterization of an acid‐thermostable glucoamylase from Aspergillus japonicus with potential application in the paper bio‐deinking

Pasin,

Betini,

de Lucas

et al. 2023

Biotechnology Progress

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Aspergillus species have been highlighted in enzyme production looking for industrial applications, notably, amylases are one of the most interesting enzymes. They are capable of hydrolyzing α‐glycosidic linkages of starch and widely used in industrial processes to produce ethanol, glucose, and fructose syrup as well as in the textiles, detergents, and paper industries applications. In this context, this work aimed at the biochemical characterization of the glucoamylase from Aspergillus japonicus and its application in the bio‐bleaching process of recycled paper. The optimum temperature and pH for the glucoamylase assay were standardized as 50°C and 5.5. After 1 h of incubation, glucoamylase retained 90% of its activity at 30–50°C. It also kept 70% of its activity in the pH range of 4.0–6.5 after an hour of incubation. The enzyme led to an increase of 30% in the relative whiteness of 10 dry grams of sulfite paper and magazine paper when applied along with commercial cellulase and 10 mM MnCl2. In addition, after the treatments, the glucoamylase recovered activity was 30%–32%, which indicates a prolonged availability of the enzyme and can considerably curtail the redundant downstream process of the recycled paper bio‐bleaching. Thus, the glucoamylase from A. japonicus has a significant role in bio‐bleaching recycled paper, reducing the necessity of hard chemicals, and improving the industrial process in an interesting economic and ecological mode.

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Section: Methodsmentioning

confidence: 99%

Section: Chemical and Instrumentsmentioning

confidence: 99%

Biochemical characterization of an acid‐thermostable glucoamylase from Aspergillus japonicus with potential application in the paper bio‐deinking

Pasin,

Betini,

de Lucas

et al. 2023

Biotechnology Progress

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“…Among the microorganisms studied, filamentous fungi are particularly interesting because they are fundamental pieces for biotechnological development [6]. In addition to the controlled application of enzymes, these microorganisms are also used to produce many other metabolites of interest for different areas of the biotechnology industry.…”

Section: Introductionmentioning

confidence: 99%

An Environmentally Safe Production of Xylanases by Fusarium sp. EA 1.3.1 Using Agroindustrial Residues: Biochemical Characterization and Potential Applications

Marinho

Nogueira

Pasin

et al. 2023

AJBGMB

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Renewable energy-related biotechnologies such as biofuels produced from low-cost residual sources that represent clean technologies have become a partial solution to environmental problems. We sought to optimize the cultivation parameters of the fungus Fusarium sp. EA1.3.1 and biochemically characterize the naturally produced xylanases from the fungus. The development of the fungus was analyzed considering the variations in the resources available and by biochemical analysis of the crude extract. The composition and duration of the cultivation, nitrogen source, carbon source, salt solution, and initial pH of the medium were evaluated. The maximum xylanolytic production was obtained in Khanna medium enriched with a CP salts solution during four days of culture using yeast extract, wheat bran, and an initial pH of 8.5 for the culture medium. The optimum temperature and pH were 65°C and 6.5, respectively, for the xylanase activity from Fusarium sp. EA 1.3.1. The enzymatic extract presented general stability at 50°C, keeping 75% of its activity after 90 minutes of incubation, and its activity decreased to 20-40% with exposure to higher temperatures (60-70°C). The enzyme also presented high stability at pH 5.0 after 90 minutes, maintaining 85% of its relative activity. Thus, the isolated fungus presents high potential for xylanase production with desired biochemical and biophysical properties for industrial application.

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Production of Amylase Enzyme by Aspergillus and Fusarium Species using Sugar Cane Bagasse

Abubakar,

Bilkisu, A.,

Shamsuddeen, U.

2024

UJMR

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Study’s Novelty/Excerpt This study presents insights into the cost-effective production of amylase enzymes using sugar cane bagasse as a substrate, showcasing a significant advancement over traditional synthetic starch substrates. By optimizing production parameters such as incubation time, substrate and inoculum concentrations, pH, and temperature, the research demonstrates the superior amylase yield of Aspergillus niger, Aspergillus flavus, and Fusarium spp. under specific conditions. This work not only highlights the potential of using waste materials for industrial enzyme production but also emphasizes the need for further exploration of the isolates' identities and the industrial applications of the extracted enzymes. Full Abstract Amylases are hydrolytic enzymes produced by some microorganisms and utilized in the hydrolysis of starch. This study aimed to determine the ability of certain fungal isolates isolated from wastes to synthesize the amylase enzyme utilizing synthetic soluble starch and sugar cane bagasse as substrates. Aspergillus niger, Aspergillus flavus, and Fusarium spp., previously identified to have amylolytic activity, were used for the research. The submerged fermentation process was used to produce amylase, with basal medium and sugar cane bagasse as substrates. The incubation time, substrate and inoculum concentrations, pH, and temperature were all optimized. The dinitrosalicylic acid reagent (DNS) technique was used to determine the activity of the amylase generated. Initial production of amylase using soluble starch (20 g (w/v)) as substrate at room temperature and pH 7.0 shows that all the isolates produce amylase better when their concentration (3%) is high but have different incubation periods of 96 hr for Aspergillus niger (8.65±0.21 U/mL/minutes) and Fusarium spp (7.15±0.07 U/mL/minutes), while Aspergillus flavus (7.30±0.14 U/mL/minutes) requires an extended incubation period of 144 hr to produce the product. Further production using sugar cane bagasse and optimization of production parameters of the isolates reveals that Aspergillus niger (4.35±0.07 U/mL/minutes) has an optimum incubation period of 120 hours, an inoculum concentration and substrate concentration of 2% each, and a pH of 6, Aspergillus flavus (6.40±0.28 U/mL/minutes) has an optimum incubation period of 144 hours, with inoculum and substrate concentration of 3% each at neutral pH, Fusarium spp (6.80±0.28 U/mL/minutes) has an optimum incubation period of 168hr., inoculum concentration of 3%, substrate concentration of 2% and a neutral pH-7 and all the isolates have their best yield at temperature of 30oC. In conclusion, using cheap and readily available sugar cane bagasse can be a more cost-effective option for expensive synthetic starch substrates used in amylase synthesis. The study suggests further investigation into the isolates' identities and the extracted enzyme's industrial application.

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Isolation of Filamentous Fungi from the Caatinga Region and Production of Amylolytic Enzymes of Great Industrial Interest

Cited by 3 publications

References 19 publications

Biochemical characterization of an acid‐thermostable glucoamylase from Aspergillus japonicus with potential application in the paper bio‐deinking

Biochemical characterization of an acid‐thermostable glucoamylase from Aspergillus japonicus with potential application in the paper bio‐deinking

An Environmentally Safe Production of Xylanases by Fusarium sp. EA 1.3.1 Using Agroindustrial Residues: Biochemical Characterization and Potential Applications

Production of Amylase Enzyme by Aspergillus and Fusarium Species using Sugar Cane Bagasse

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