Abstract:Fibronectin was isolated from human blood plasma by affinity chromatography
on immobilized Physiogel, a plasma expander made by chemical degradation of gelatin.
Binding of fibronectin to Physiogel is weaker than to gelatin; elution could therefore be
performed under rather mild conditions: at pH 6.5 and 30°C. Loading of the sample at low
temperature increases the capacity of the affinity column.
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