Isolation of fatty acids with anticancer activity fromProtaetia brevitarsis Larva

Yoo, Yung Choon; Shin, Byung-Hoon; Hong, Jang Hee; Lee, Junglim; Chee, Hee-Youn; Song, Kyung-Sik; Lee, Kyung‐Bok

doi:10.1007/bf02977619

Cited by 80 publications

(67 citation statements)

References 20 publications

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“…One molecular target of palmitic acid in tumour cells is DNA topoisomerase I, however, interestingly, it does not affect DNA topoisomerase II, suggesting that palmitic acid may be a lead compound of anticancer drugs (Harada et al, 2002). Recently, the in vitro cytotoxic activity of fatty acids and methyl esters against various cancer cells was reported (Takeara et al, 2007;Yoo et al, 2007). In particular, a methyl esters mixture containing methyl palmitate and methyl stearate was investigated for mechanisms involved in the cytotoxic activity.…”

Section: Resultsmentioning

confidence: 99%

In vitro Cytotoxic Activity of Salsola oppositifolia Desf. (Amaranthaceae) in a Panel of Tumour Cell Lines

Tundis

Loizzo

Bonesi

et al. 2008

Zeitschrift Für Naturforschung C

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The aim of the present study was to evaluate for the first time the in vitro cytotoxic activity of fractions and isolated flavonols from Salsola oppositifolia Desf. (Amaranthaceae). The nhexane fraction demonstrated an effective cytotoxic activity on the large lung carcinoma and amelanotic melanoma cell lines with IC 50 values of 19.1 μg/ml and 24.4 μg/ml, respectively. Also the dichloromethane fraction exhibited cytotoxic activity against COR-L23 (IC 50 30.4 μg/ml) and C32 (IC 50 33.2 μg/ml) cells, while the EtOAc fraction demonstrated a selective cytotoxic activity against MCF-7 cells (IC 50 67.9 μg/ml). The major active constituents of this fraction were isorhamnetin-3-O-glucoside (1) and isorhamnetin-3-O-rutinoside (2), which showed an interesting activity against the cell line MCF-7 with IC 50 values of 18.2 and 25.2 μg/ml, respectively. Compound 2 exhibited a strong activity against the hormonedependent prostate carcinoma LNCaP cell line with an IC 50 of 20.5 μg/ml. Constituents of S. oppositifolia were identified by GC-MS and NMR analyses.

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Section: Resultsmentioning

confidence: 99%

In vitro Cytotoxic Activity of Salsola oppositifolia Desf. (Amaranthaceae) in a Panel of Tumour Cell Lines

Tundis

Loizzo

Bonesi

et al. 2008

Zeitschrift Für Naturforschung C

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“…Aside from its antipsychotic usage, palmitic acid also like decanoic acid to use in depot medication particularly haloperidol decanoate. The highlighting part of palmitic acid is that it has been studied as anti-tumor [43] and anticancer [44] activities. This compound was suggested by Benoit et al [45] to play a role in weight regulation as in vivo study from rats fed a diet of palmitic acid shows suppression of the body natural appetite suppressing signals from leptin and insulin [45].…”

Section: Discussionmentioning

confidence: 99%

Study of Dendrophthoe Pentandra Ethyl Acetate Extract as Potential Anticancer Candidate on Safety and Toxicity Aspects

Sul’ain¹,

Yee²,

Fauzi³

et al. 2017

JAPLR

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In the present study, the safety and toxicity of Dendrophthoe pentandra (DP) extracted using ethyl acetate were investigated. DP ethyl acetate (DPEA) extract was screened for its phytochemical constituents using standard procedure and active compounds present in DPEA extract were determined using gas chromatographymass spectrometry (GC-MS), the toxicity of DPEA extract was investigated using brine shrimp lethality assay (BSLA) while the heavy metal content of DP plant was detected using atomic absorption spectrophotometer (AAS) and the cytotoxicity effect of DPEA extract on selected cell lines (HeLa, L929, Glioma, MD-AMB 231, Hep G2 and MCF-7) was determined through MTT assay. The results showed that DPEA extract consist of wide range of bioactive compounds including tannins, saponins, flavonoids and alkaloids. The crude extract contained 1.33% alkaloid, 2.67% flavonoid and 14.87±0.2µg/g total phenolic content. Heavy metals such as copper, zinc and manganese were present below the maximum permissible level. GC-MS result revealed the presence of 39 compounds and out of that, only decanoic acid, palmitic acid, linolenic acid and betasitosterol were suggested to contribute to the medicinal use of DP. The LC 50 of DPEA extract was underdetermined and was predicted above 1000 ppm and DPEA extract showed antiproliferative activity on MCF-7 and L929 with IC 50 of 4.72±0.52µg/ml and 18.12±3.46µg/ml respectively. Thus, the result of the present study revealed that DP plant is relatively safe and can be considered as possible candidates with promising potency to be developed as new chemotherapeutic agent.

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“…To rapidly perform simultaneous identification and quantification of infectious agents, the concentration of pathogen genomic DNA could be calculated based on fluorescence intensity in Multiplex Ligation-dependent Probe Amplification (MPLA), which is a variation of multiplex PCR that permits amplification of multiple et Suh and Kang, 2012;Yeo et al, 2013;Yoo et al, 2007;Youn et al, 2014 (Coleoptera: Scarabaeidae) are extensively farmed in Korea (Aronson et al, 1986;Choi et al, 2003; Jander et al, 2000; Lee et al, 1997). Several reports indicate that these beetles can be used as functional food because of their antioxidant (Suh and Kang, 2012;Suh et al, 2010), anti-hepatofibrotic, anti-cancer, and anti-diabetic properties (Koyama et al, 2006;Miyanoshita et al, 1996;Taketa et al, 1986;Yamada et al, 2004;Yeo et al, 2013;Yoo et al, 2007;Yoon et al, 2003;Youn et al, 2012), and in Korea, P. b. seulensis and A. dichotoma are grown in farms.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous detection of fungal, bacterial, and viral pathogens in insects by multiplex PCR and capillary electrophoresis

Kwak¹,

Nam²,

Choi³

et al. 2015

International Journal of Industrial Entomology

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Beetles Protaetia brevitarsis seulensis Kolbe (Coleoptera: Cetoniidae) and Allomyrina dichotoma Linn. (Coleoptera: Scarabaeidae) are widely used in traditional medicine, and the number of insect-rearing farms is increasing in South Korea. The purpose of this study was to establish a multiplex PCR-based assay for rapid simultaneous detection of multiple pathogens causing insect diseases. Six insect parasites such as fungi Beauveria bassiana (Bals.-Criv.) Vuill. (Hypocreales: Cordycipitaceae) and Metarhizium anisopliae (Metschn.) Sorokin (Hypocreales: Clavicipitaceae), bacteria Bacillus thuringiensis Berliner (Bacillales: Bacillaceae), Pseudomonas aeruginosa Migula (Pseudomonadales: Pseudomonadaceae), and Serratia marcescens Bizio (Enterobacteriales: Enterobacteriaceae), and Oryctes rhinoceros nudivirus were chosen based on the severity and incidence rate of insect diseases in South Korea. Pathogen-specific primers were designed and successfully applied for simultaneous detection of multiple infectious agents in farm-bred insects P. b. seulensis and A. dichotoma using multiplex PCR and high resolution capillary electrophoresis. Our results indicate that multiplex PCR is an effective and time-saving method for simultaneous detection of multiple infections in insects, and the QIAxcel capillary electrophoresis system is useful for quantitative evaluation of the individual impact of each infectious agent on the severity of insect disease. The approach designed in this study can be utilized for rapid and accurate diagnostics of infection in insect farms.© 2015 The Korean Society of Sericultural Sciences Int. J. Indust. Entomol. 30(2), 64-74 (2015) IntroductionFollowing a global trend of using natural products from insects, the Korean government has been encouraging the development of insect resources for environmental and dietary purposes, traditional medicine, and as pets (Kim et al., 2013 Vol. 30, No. (1), pp. 64-74 (2015) Pseudomonas aeruginosa is reported as a virulent bacterial pathogen not only for insects but also for mammals (Jander et al., 2000), where it causes infection of the respiratory tract, especially in cystic fibrosis patients (Spiker et al., 2004). Similar to Pseudomonas aeruginosa, Serratia marcescens is known as an opportunistic bacterium (El-Aasar et al., 2013); it is also used to control apple maggot fly population and as a source of insecticidal agents such as toxin luxA (Hejazi and Falkiner, 1997; Lauzon et al., 2003). Oryctes rhinoceros virus Rhabdionvirus oryctes Hüger was first discovered in oil palms in Malaysia 40 years ago and is currently used for the control of rhinoceros beetle population in Malaysia (Huger, 2005;Ramle et al., 2005).We have identified this virus in an A. dichotoma-breeding farm in 2014 in Korea (Lee, 2015).Extensive large-scale insect breeding has resulted in frequent occurrence of insect infection due to multiple pathogens. Here, we developed a multiplex PCR-based approach that allowed simultaneous detection of six insect pathogens using specific primer se...

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Isolation of fatty acids with anticancer activity fromProtaetia brevitarsis Larva

Cited by 80 publications

References 20 publications

In vitro Cytotoxic Activity of Salsola oppositifolia Desf. (Amaranthaceae) in a Panel of Tumour Cell Lines

In vitro Cytotoxic Activity of Salsola oppositifolia Desf. (Amaranthaceae) in a Panel of Tumour Cell Lines

Study of Dendrophthoe Pentandra Ethyl Acetate Extract as Potential Anticancer Candidate on Safety and Toxicity Aspects

Simultaneous detection of fungal, bacterial, and viral pathogens in insects by multiplex PCR and capillary electrophoresis

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