Isolation of exosome from the culture medium of Nasopharyngeal cancer (NPC) C666-1 cells using inertial based Microfluidic channel

Teoh, Boon Yew; Lim, Yang Mooi; Wang, Chong; Subramaniam, Menaga; Tan, Zi Zhang; Misran, Misni; Suk, Vicit Rizal Eh; Lo, Kwok‐Wai; Lee, Poh Foong

doi:10.1007/s10544-022-00609-z

Cited by 7 publications

(5 citation statements)

References 36 publications

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“…These microfluidic devices enable efficient extraction and detection of specific exosomes, making them suitable for use in clinical applications [ 179 ]. In relation to NPC, a successful separation of exosomes from the culture medium of C666-1 cell line using a microfluidic-based approach has been achieved by Teoh et al [ 180 ]. However, it is important to note that the scalability of capture-based techniques is limited by cost constraints.…”

Section: Limitations Of Exosomes In Clinical Use and Future Directionsmentioning

confidence: 99%

Unfolding the Complexity of Exosome–Cellular Interactions on Tumour Immunity and Their Clinical Prospects in Nasopharyngeal Carcinoma

Chak,

Kam,

Choi

et al. 2024

Cancers

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Nasopharyngeal carcinoma (NPC) is an epithelial malignancy situated in the posterolateral nasopharynx. NPC poses grave concerns in Southeast Asia due to its late diagnosis. Together with resistance to standard treatment combining chemo- and radiotherapy, NPC presents high metastatic rates and common recurrence. Despite advancements in immune-checkpoint inhibitors (ICIs) and cytotoxic-T-lymphocytes (CTLs)-based cellular therapy, the exhaustive T cell profile and other signs of immunosuppression within the NPC tumour microenvironment (TME) remain as concerns to immunotherapy response. Exosomes, extracellular vesicles of 30–150 nm in diameter, are increasingly studied and linked to tumourigenesis in oncology. These bilipid-membrane-bound vesicles are packaged with a variety of signalling molecules, mediating cell–cell communications. Within the TME, exosomes can originate from tumour, immune, or stromal cells. Although there are studies on tumour-derived exosomes (TEX) in NPC and their effects on tumour processes like angiogenesis, metastasis, therapeutic resistance, there is a lack of research on their involvement in immune evasion. In this review, we aim to enhance the comprehension of how NPC TEX contribute to cellular immunosuppression. Furthermore, considering the detectability of TEX in bodily fluids, we will also discuss the potential development of TEX-related biomarkers for liquid biopsy in NPC as this could facilitate early diagnosis and prognostication of the disease.

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Section: Limitations Of Exosomes In Clinical Use and Future Directionsmentioning

confidence: 99%

Unfolding the Complexity of Exosome–Cellular Interactions on Tumour Immunity and Their Clinical Prospects in Nasopharyngeal Carcinoma

Chak,

Kam,

Choi

et al. 2024

Cancers

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“…Based on its good biocompatibility, air permeability, and transparency, PDMS is the main material for microfluidic chips. [11,13,106,124,160,179] However, there are certain limitations of PDMS as well. The hydrophobicity of the PDMS surface can easily cause non-specific adsorption of drugs and biomolecules, which affects the accuracy of the experiment.…”

Section: Biological Productionmentioning

confidence: 99%

“…The emergence of microfluidic technology has provided a broad stage for the investigation of cellular processes through real-time in situ monitoring of biomarkers during cell culture. Microfluidic platforms, also known as lab-on-a-chip, [8] provide an ideal tool for the real-time in situ monitoring of biomarkers at the cellular level, [9] and are thus widely used in cell culture, [10] cell differentiation, [11] cell migration, [12] and cell-tocell communication. [13] The first microfluidic platform dates back to 1975, when James et al reported a gas chromatographic air analyzer fabricated on a silicon wafer.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Platforms for Real‐Time In Situ Monitoring of Biomarkers for Cellular Processes

Lou,

Yang,

Hou

et al. 2023

Advanced Materials

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Cellular processes are mechanisms carried out at the cellular level (within or among more than one cell interactions) that are aimed at guaranteeing the stability of the organism they comprise. The investigation of cellular processes is key to understanding cell fate, understanding pathogenic mechanisms, and developing new therapeutic technologies. Microfluidic platforms are thought to be the most powerful tools among all methodologies for investigating cellular processes because they can integrate almost all types of the existing intracellular and extracellular biomarker‐sensing methods and observation approaches for cell behavior, combined with precisely controlled cell culture, manipulation, stimulation, and analysis. Most importantly, microfluidic platforms can realize real‐time in situ detection of secreted proteins, exosomes, and other biomarkers produced during cell physiological processes (adhesion, differentiation, migration, apoptosis, and cell‐to‐cell communication), thereby providing the possibility to draw the whole picture for a cellular process. In addition, these can be used to study the process of cell or tissue response to changes in the microenvironment (drugs, physical signals, or types and quantities of surrounding cells). Owing to their advantages of high throughput, low sample consumption, and precise cell control, microfluidic platforms with real‐time in situ monitoring characteristics are widely being used in cell analysis (cellular heterogeneity analysis, cell sorting, and cellular marker detection), disease diagnosis, pharmaceutical research, and biological production. This review focuses on the basic concepts, recent progress, and application prospects of microfluidic platforms for real‐time in situ monitoring of biomarkers in cellular processes.This article is protected by copyright. All rights reserved

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“…In fact, F i acts to drive the particles orthogonally to the flow direction inside the microchannel in a manner that strongly depends on particle dimension D (F i ∝ D 4 ). Therefore, by properly tuning the channel size and flow rates, it is necessary to focus the particles according to their size [50][51][52][53][54][55]. A particular configuration consists of spiral channels that strongly favor the lateral migration of particles with different velocities depending on size [56], as used by Tay et al for rapid isolation from a whole blood sample at 80 µL/min, despite reaching a poor recovery efficiency (20-60%) [57].…”

Section: Passive Approachesmentioning

confidence: 99%

“…Filtration [38][39][40][41][42][43][44][45]150,151] Micro-/nano-filtration process by porous membranes inside chip Deterministic lateral displacement [46,[60][61][62] Particle distribution in size by lateral forces conveyed by ordered array of posts Inertial force [50][51][52][53][54][55]77] Viscoelastic force [67][68][69][70][71][72] Imbalance of inertial forces or of shear forces in non-Newtonian viscoelastic fluid…”

Section: Physical: Passivementioning

confidence: 99%

Microfluidic Strategies for Extracellular Vesicle Isolation: Towards Clinical Applications

et al. 2022

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Extracellular vesicles (EVs) are double-layered lipid membrane vesicles released by cells. Currently, EVs are attracting a lot of attention in the biological and medical fields due to their role as natural carriers of proteins, lipids, and nucleic acids. Thus, they can transport useful genomic information from their parental cell through body fluids, promoting cell-to-cell communication even between different organs. Due to their functionality as cargo carriers and their protein expression, they can play an important role as possible diagnostic and prognostic biomarkers in various types of diseases, e.g., cancers, neurodegenerative, and autoimmune diseases. Today, given the invaluable importance of EVs, there are some pivotal challenges to overcome in terms of their isolation. Conventional methods have some limitations: they are influenced by the starting sample, might present low throughput and low purity, and sometimes a lack of reproducibility, being operator dependent. During the past few years, several microfluidic approaches have been proposed to address these issues. In this review, we summarize the most important microfluidic-based devices for EV isolation, highlighting their advantages and disadvantages compared to existing technology, as well as the current state of the art from the perspective of the use of these devices in clinical applications.

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Isolation of exosome from the culture medium of Nasopharyngeal cancer (NPC) C666-1 cells using inertial based Microfluidic channel

Cited by 7 publications

References 36 publications

Unfolding the Complexity of Exosome–Cellular Interactions on Tumour Immunity and Their Clinical Prospects in Nasopharyngeal Carcinoma

Unfolding the Complexity of Exosome–Cellular Interactions on Tumour Immunity and Their Clinical Prospects in Nasopharyngeal Carcinoma

Microfluidic Platforms for Real‐Time In Situ Monitoring of Biomarkers for Cellular Processes

Microfluidic Strategies for Extracellular Vesicle Isolation: Towards Clinical Applications

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