Isolation of Erwinia Amylovora From Blighted Plums (Prunus Domestica) and Potato Roses (Rosa Rugosa)

Vanneste, J. L.; Vermeulen, Marcele; Lex, S.; Berger, F.; Hale, Chris; Mitchell, Richard R.

doi:10.17660/actahortic.2002.590.10

Cited by 15 publications

(12 citation statements)

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“…However, symptom development on cherry and plum fruits indicates that those plants could be potential hosts of E. amylovora. Moreover, fire blight on European plum was recorded in Germany in 2002 (Vanneste et al, 2002) and in Hungary in 2011 (Végh et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia

et al. 2018

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The diversity of 30 Erwinia amylovora strains, isolated from quince, pear and apple trees on 14 localities in Serbia, was studied using bacteriological and molecular methods. In pathogenicity tests, all strains caused necrosis and oozing of bacterial exudate on inoculated immature pear, cherry and plum fruits, and induced hypersensitive reaction in tobacco leaves. The studied strains were Gram and oxidase negative, non-fluorescent, levan and catalase positive and facultatively anaerobic. The strains did not reduce nitrates, but utilized citrate and produced acid from sorbitol, hydrolyzed gelatine, produced reducing substances from sucrose and grew in the presence of 5% NaCl, but not at 36ºC. Identity of the strains was confirmed by conventional and nested PCR methods. Rep-PCR with REP, ERIC and BOX primers resulted in amplification of several DNA fragments respectively, but showed no variation within the strains. However, different genetic profiles were obtained with RAPD-PCR by using six primers which enabled differentiation of the strains into four groups. Genetic differences between the studied strains did not correlate with the host plants, geographical origin or year of isolation.

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Section: Discussionmentioning

confidence: 99%

Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia

et al. 2018

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“…). Further reports describe detection of E. amylovora on symptomless sweet cherry blossoms (Moltmann & Viehrig, ), and its isolation from plum tissue with symptoms (Mohan & Thomson, ; Vanneste et al ., ), and strawberry (Atanasova et al ., ), all of which are members of the Rosaceae but hitherto not classified as host plants. There has been no study regarding the epiphytic development of E. amylovora on non‐host plants and their potential role in dissemination of fire blight bacteria.…”

Section: Discussionmentioning

confidence: 99%

Alternative inoculum sources for fire blight: the potential role of fruit mummies and non‐host plants

et al. 2015

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Fire blight is the most damaging bacterial disease in apple production worldwide. Cankers and symptomless infected shoots are known as sites for the overwintering of Erwinia amylovora, subsequently providing primary inoculum for infection in the spring. In the present work, further potential sources of inoculum were investigated. Real-time PCR assays covering a 3-year-period classified 19Á9% of samples taken from fruit mummies as positive. Bacterial abundance in fruit mummies during autumn, winter and spring was up to 10 9 cells per gram of tissue and correlated well with later infection rates of blossoms. Blossoms of non-host plants growing close to infected trees were also shown to be colonized by E. amylovora and to enable epiphytic survival and propagation of bacteria. The results indicate a potential role of fruit mummies and buds in overwintering and as a source of primary inoculum for dissemination of the pathogen early in the growing season. Non-host blossoms may also serve as an inoculum source in the build-up of the pathogen population. Both aspects may contribute significantly to the epidemiology of E. amylovora. The significance of infected rootstocks as an inoculum source is also discussed. Fruit mummies might be used to determine pathogen pressure in an orchard before the beginning of the blooming period.

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“…The Maloideae subfamily's species are frequently affected and are also of high economic importance because Pyrus, Malus, Cydonia, Eryobotria, Cotoneaster, Crataegus, Pyracantha, and Sorbus species are the most common hosts. Fire blight has also been described in raspberry (Rubus idaeus) (Starr et al 1951) and, more recently, in Rosa rugosa in Germany (Vanneste et al 2002); both belong to the Rosoideae subfamily. In some cases, the bacterium has been reported in the United States (USA) and in South Germany as causing similar symptoms in members of Amygdaloideae, such as in Japanese plum (Prunus salicina) (Mohan and Thomson 1996), European plum (Prunus domestica) (Vanneste et al 2002), and Pluot Ò (plum 9 apricot hybrid) trees naturally infected under high inoculum pressure (Mohan 2007).…”

Section: Erwinia Amylovoramentioning

confidence: 97%

“…Fire blight has also been described in raspberry (Rubus idaeus) (Starr et al 1951) and, more recently, in Rosa rugosa in Germany (Vanneste et al 2002); both belong to the Rosoideae subfamily. In some cases, the bacterium has been reported in the United States (USA) and in South Germany as causing similar symptoms in members of Amygdaloideae, such as in Japanese plum (Prunus salicina) (Mohan and Thomson 1996), European plum (Prunus domestica) (Vanneste et al 2002), and Pluot Ò (plum 9 apricot hybrid) trees naturally infected under high inoculum pressure (Mohan 2007). A plum isolate of E. amylovora has also been reported to cause symptoms after inoculation in other species in this subfamily, specifically, in cultivars of peach (P. persica), nectarine (P. persica var.…”

Section: Erwinia Amylovoramentioning

confidence: 97%

Erwinia spp. from pome fruit trees: similarities and differences among pathogenic and non-pathogenic species

Palacio-Bielsa

Roselló

Llop

et al. 2011

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The number of described pathogenic and nonpathogenic Erwinia species associated with pome fruit trees, especially pear trees, has increased in recent years, but updated comparative information about their similarities and differences is scarce. The causal agent of the fire blight disease of rosaceous plants, Erwinia amylovora, is the most studied species of this genus. Recently described species that are pathogenic to pear trees include Erwinia pyrifoliae in Korea and Japan, Erwinia spp. in Japan, and Erwinia piriflorinigrans in Spain. E. pyrifoliae causes symptoms that are indistinguishable from those of fire blight in Asian pear trees, Erwinia spp. from Japan cause black lesions on several cultivars of pear trees, and E. piriflorinigrans causes necrosis of only pear blossoms. All these novel species share some phenotypic and genetic characteristics with E. amylovora. Non-pathogenic Erwinia species are Erwinia billingiae and Erwinia tasmaniensis that have also been described on pome fruits; however, less information is available on these species. We present an updated review on the phenotypic and molecular characteristics, habitat, pathogenicity, and epidemiology of E. amylovora, E. pyrifoliae, Erwinia spp. from Japan, E. piriflorinigrans, E. billingiae, and E. tasmaniensis. In addition, the interaction of these species with pome fruit trees is discussed.

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Isolation of Erwinia Amylovora From Blighted Plums (Prunus Domestica) and Potato Roses (Rosa Rugosa)

Cited by 15 publications

References 0 publications

Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia

Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia

Alternative inoculum sources for fire blight: the potential role of fruit mummies and non‐host plants

Erwinia spp. from pome fruit trees: similarities and differences among pathogenic and non-pathogenic species

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