Isolation of ecdysterone from indian plants

Banerji, Asoke; Chintalwar, G. J.; Joshi, N.K.; Chadha, M. S.

doi:10.1016/s0031-9422(00)97227-3

Cited by 52 publications

(25 citation statements)

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“…Roots extracts yielded ecdysterone (3), 4,5) methyl palmitate (4), stigmast-6-en-3-O-b-(Dglicopiranoside) (5) 5,6) and stigmasterol. To the best of our knowledge 1, 2, 4 and 5 were isolated for the first time from G. celosioides.…”

Section: Resultsmentioning

confidence: 99%

“…2,3) There are few phytochemical and pharmacological screening report on this genus. 1,4) In this paper we deal with the isolation, structural elucidation of constituents, antimicrobial activity and quantitative determination of benzoic acid derivative by TLC-densitometry. …”

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“…Fractions were collected and grouped according to the results of TLC. Beginning fractions gave methyl palmitate (4) and others fractions were recrystallized with acetone to afford an saponin: stigmast-6-en-3-O-b-(D-glicopiranoside) (5). Another fraction was purified by silica gel (silica gel 60) column chromatography to afford stigmasterol.…”

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Antimicrobial Screening and Quantitative Determination of Benzoic Acid Derivative of Gomphrena celosioides by TLC-Densitometry

et al. 2004

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The genus Gomphrena (family Amaranthaceae) comprises approximately 120 species found in the Americas, Australia, and Indo-Malaysia; 46 species occur in Brazil, in savanna vegetation (cerrado), napeadic grassland (campo limpo), high altitude grassland (campo rupestre), and caatinga; only a few species are found in forest.1) A number of Brazilian Gomphrena species are employed in the treatment of bronchial affections, diarrhea, and fever, and as an analgesic, tonic, or carminative.1) This species show antimalarial and diuretic activities. 2,3) There are few phytochemical and pharmacological screening report on this genus. 1,4) In this paper we deal with the isolation, structural elucidation of constituents, antimicrobial activity and quantitative determination of benzoic acid derivative by TLC-densitometry. ExperimentalPlant Material Gomphrena celosioides was collected in Paranaiba, Mato Grosso do Sul State, in December 1994 and authenticated by Prof. Josafá Carlos de Siqueira. A voucher specimen is deposited in the herbarium of Pontifical Catholic University, Rio de Janeiro (SCAB 4051).Extraction and Purification Dry powdered aerial parts (6 kg) and roots (3 kg) were macerated successively with hexane, ethanol and methanol.The ethanolic extract of the aerial parts (281.0 g) was submitted to partition successively with hexane and chloroform yielding crude extract: 1.6 and 20.0 g respectively. The extracts were chromatographed respectively over a vacuum column filled with silica gel (silica gel H, Merck). The elution started with hexane by using vacuum. Then EtOAc was gradually added until the eluant was pure EtOAc. Then methanol was gradually added until the final eluant was pure methanol. Fractions were collected and grouped according to the results of TLC. The combined fractions of the hexanic extract were submitted to analysis by GC with authentic substances. Identification of stigmasterol, sitosterol and campesterol was carried out by comparing their retention times with those of the standards. Final separation of the chloroformic extract by HPLC on a silica gel RP-18 (20ϫ250 mm) with MeOH : H 2 O (3 : 7) gave 4-hydroxy-benzoic acid (1) and 4-hydroxy-3-methoxy-benzoic acid (2).The methanolic extract of the roots (60.7 g) was submitted to partition successively with chloroform and n-butanol. The butanolic phase was evaporated and chromatographed over a column filled with silica gel (silica gel H, Merck). The elution started with hexane by using pressure. Then EtOAc was gradually added until the eluant was pure EtOAc. Then methanol was gradually added until the final eluant was pure methanol. One hundred and fifty milliliters fractions were collected and grouped according to the results of TLC. Final purification by PTLC on a silica gel C-18 (20ϫ20 cm) eluting with MeOH : H 2 O (3 : 7) gave ecdysterone (3).The ethanolic extract of the roots (54.3 g) was fractionated by silica gel (silica gel G-60) column chromatography using reducing pressure. The elution system followed the procedure above-mentioned. Fractions ...

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Section: Resultsmentioning

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Antimicrobial Screening and Quantitative Determination of Benzoic Acid Derivative of Gomphrena celosioides by TLC-Densitometry

et al. 2004

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“…20 There is little phytochemical and pharmacological screening report on this species. 16,17,18,19,20,21 The phytochemical review on Gomphrena serrata indicates that there were no reports available for the presence and detection of kaempferol from the plant species. Kaempferol was reported for the first time from the plant species.…”

Section: Detection and Quantification Of Major Phytochemical Markers mentioning

confidence: 99%

Detection and Quantification of Major Phytochemical Markers for Standardization of Talinum Portulacifolium, Gomphrena Serrata, Alternanthera Sessilis and Euphorbia Heterophylla by HPLC

Mamillapalli¹,

Rahaman²,

Rani³

2018

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Back ground: High-performance Liquid Chromatography is one of the major analytical techniques used in the quality control of phytochemicals. Objective: This research article presents the development of HPLC method to detect and quantify the major marker components, kaempferol, and quercetin from four plant species. Materials and Methods: HPLC method was developed for the qualitative and quantitative analysis of plant extracts by using orthophosphoric acid and methanol (95:5) at 370 nm for kaempferol, methanol and orthophosphoric acid (60:40) at 262nm for quercetin. Results: Kaempferol was detected from the hydro alcoholic extracts of Talinum portulacifolium leaves (RT 13.720, concentration 1.08 mg/ml) and flowers of Gomphrena serrata (RT 13.758, concentration 2.13mg/ml). Kaempferol was reported for the first time from Gomphrena serrata. Quercetin was separated and identified from the hydro alcoholic extracts Alternanthera sessilis stems (RT 6.503, concentration 0.01mg/ml). The hydroalcoholic extract of Euphorbia heterophylla stems (RT 6.588, concentration 0.01mg/ml) was also evaluated for the presence of quercetin. Conclusion: The method developed is very useful tool for qualifying and quantifying the plant specimens as well as their extracts.

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“…The decoction of whole plant is given in fertility regulating, while roots and shoots together given for piles and roots chewed in cough [4][5][6] . Phyto compounds like 3-(4-hydroxyphenyl) methylpropenoate, Aurantiamides, Aurantiamide acetate and Ecdysterone were previously isolated from the plant Gomphrena celosioides while one methoxy derivative of kaempferol was identified in Nothosaerva brachiata [7][8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Quercetin From Amaranthaceae Family Plants

Pk¹,

Srivastava²

2017

Int. J. Res. Ayurveda Pharm.

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The two medicinal plants viz. Nothosaerva brachiata and Gomphrena celosioides belonging to amaranthaceae family were chosen to isolate flavonoid compounds since no scientific study reported so far. Quercetin was isolated from N. brachiata and G. celosioides. The soxhlet extraction was carried out for dried root powder of both the plants with petroleum ether, chloroform and methanol successively. The methanolic fraction so obtained was successively extracted with petroleum ether, diethyl ether and ethyl acetate. The ethyl acetate fraction was hydrolysed with 7% H2SO4, kept for hydrolysis for 7 hours to obtain quercetin. It was then extracted with ethyl acetate to obtain crude quercetin. The isolated compounds were subjected for characterization by TLC, HPTLC and HRLC-MS. The isolated compound GQ, N2Q were identified as "Quercetin". This is the first report of isolation of said phyto compounds from these plants.

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Isolation of ecdysterone from indian plants

Cited by 52 publications

References 6 publications

Antimicrobial Screening and Quantitative Determination of Benzoic Acid Derivative of Gomphrena celosioides by TLC-Densitometry

Antimicrobial Screening and Quantitative Determination of Benzoic Acid Derivative of Gomphrena celosioides by TLC-Densitometry

Detection and Quantification of Major Phytochemical Markers for Standardization of Talinum Portulacifolium, Gomphrena Serrata, Alternanthera Sessilis and Euphorbia Heterophylla by HPLC

Isolation and Characterization of Quercetin From Amaranthaceae Family Plants

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