1985
DOI: 10.1111/j.1471-4159.1985.tb05463.x
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Isolation of Diosgenin[(25R)‐Spirost‐5‐en‐3β‐o1] from a Lysate of a New Hypotensive Phospholipid Occurring in Bovine Brain

Abstract: Diosgenin[(25R)-spirost-5-en-3 beta-ol] and cholesterol were detected as trimethylsilyl derivatives by gas chromatography-mass spectrometry in an alkaline lysate of a new hypotensive phospholipid from the lipid fraction of bovine brain. These steroids seemed to be present in the hypotensive phospholipid fraction in some bound forms.

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“…Sapogenins and aglycones of steroidal glycoalkaloids were analyzed using GC-MS in the following conditions: oven temperature programmed from 220 to 320°C at 8°C min -1 and then kept for 20 min at 320°C; injector and transfer line temperatures were set at 320°C. Compounds were identified basing on their mass spectra and retention parameters (Akahori et al 1971;Laurila et al 1999;Lee et al 2001;Taylor et al 1997;Tsukatani et al 1985). Commercially available solasodine standard was used to confirm its presence in S. macrocarpon and S. dasyphyllum samples.…”
Section: Saponin and Glycoalkaloid Analysismentioning
confidence: 99%
“…Sapogenins and aglycones of steroidal glycoalkaloids were analyzed using GC-MS in the following conditions: oven temperature programmed from 220 to 320°C at 8°C min -1 and then kept for 20 min at 320°C; injector and transfer line temperatures were set at 320°C. Compounds were identified basing on their mass spectra and retention parameters (Akahori et al 1971;Laurila et al 1999;Lee et al 2001;Taylor et al 1997;Tsukatani et al 1985). Commercially available solasodine standard was used to confirm its presence in S. macrocarpon and S. dasyphyllum samples.…”
Section: Saponin and Glycoalkaloid Analysismentioning
confidence: 99%