Isolation of Decidual Macrophages and Hofbauer Cells from Term Placenta—Comparison of the Expression of CD163 and CD80

Abstract

(1) Background: Placental immune cells are playing a very important role in a successful placentation and the prevention of pregnancy complications. Macrophages dominate in number and relevance in the maternal and the fetal part of the placenta. The evidence on the polarization state of fetal and maternal macrophages involved in both, healthy and pregnancy-associated diseases, is limited. There is no representative isolation method for the direct comparison of maternal and fetal macrophages so far. (2) Material and Methods: For the isolation of decidual macrophages and Hofbauer cells from term placenta, fresh tissue was mechanically dissected and digested with trypsin and collagenase A. Afterwards cell enrichment was increased by a Percoll gradient. CD68 is represented as pan-macrophage marker, the surface markers CD80 and CD163 were further investigated. (3) Results: The established method revealed a high cell yield and purity of the isolated macrophages and enabled the comparison between decidual macrophages and Hofbauer cells. No significant difference was observed in the percentage of single CD163+ cells in the distinct macrophage populations, by using FACS and immunofluorescence staining. A slight increase of CD80+ cells could be found in the decidual macrophages. Considering the percentage of CD80+CD163− and CD80−CD163+ cells we could not find differences. Interestingly we found an increased number of double positive cells (CD80+CD163+) in the decidual macrophage population in comparison to Hofbauer cells. (4) Conclusion: In this study we demonstrate that our established isolation method enables the investigation of decidual macrophages and Hofbauer cells in the placenta. It represents a promising method for direct cell comparison, enzyme independently, and unaffected by magnetic beads, to understand the functional subsets of placental macrophages and to identify therapeutic targets of pregnancy associated diseases.

Proteomic studies of VEGFR2 in human placentas reveal protein associations with preeclampsia, diabetes, gravidity, and labor

“…In diabetic patients, higher MDMX levels (Supplementary Table 4) seem to correspond to the higher number of cells stained with CD163 [64], a marker of HC [42]. It is not known if MDMX upregulation and higher HC numbers mark a metabolic shift towards diabetes.…”

“…3C). CD163 [36,42], a marker for placental macrophages [43], stained the cytoplasm of the HC (Fig. 3D).…”

Tumor cell-derived exosomal miR-193b-3p promotes tumor-associated macrophage activation to facilitate nasopharyngeal cancer cell invasion and radioresistances

“…Peripheral blood samples were collected from 21 patients with NPC. Peripheral blood mononuclear leukocytes (PBMCs) were collected using Ficoll-Hypaque density centrifugation, and CD68 + macrophages were purified as blood-derived macrophages (BDMs) using flow cytometry [ 29 , 30 ].…”

Histological Properties of Placentas in Acute Herpes Simplex Virus Infection Affecting Genitals

“…Scale bar: Г -50 µm, the rest -20 µm. [15,19]. Согласно литературным данным, наличие молекулярных паттернов, ассоциированных с патогеном, вызванных микроорганизмами (PAMPs) или молекулярных паттернов, ассоциированных с повреждением хозяина (DAMPs), запускает сигнальный каскад, что приводит к увеличению экспрессии цитокинов, хемокинов и интерферонов [20].…”