Isolation of CYP17 I, 3β-HSD and AR Genes from Spotted Sea Bass (Lateolabrax maculatus) Testis and Their Responses to Hormones and Salinity Stimulations

Chi, Meili; Ni, Meng; Jia, Yongyi; Gu, Zhenxin; Wen, Haishen

doi:10.1007/s11802-019-3786-9

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…The expression of CYP17 mRNA has been observed in several nonmammalian vertebrate species. In the spotted sea bass, Lateolabrax maculatus , CYP17 mRNA was present in steroidogenic tissues and strong ISH signals were mostly found in the area of Leydig cells in the testis (Chi et al, 2019). In the zebrafish, CYP17A1 mRNA was prominently expressed in male gonads (Hinfray et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Steroidogenic potential of the gonad during sex differentiation in the rice field frog Hoplobatrachus rugulosus (Anura: Dicroglossidae)

et al. 2023

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Prior studies demonstrated that gonadal differentiation in the rice field frog, Hoplobatrachus rugulosus, was of an undifferentiated type since all individuals had ovaries at complete metamorphosis. However, the steroidogenic potential of the gonad is still unknown. In this study, H. rugulosus were obtained by stimulating fertilization in the laboratory under natural light and temperature conditions. The gonads were collected and their steroidogenic potential was evaluated by determining the expression level of messenger RNA (mRNA) encoding for cytochrome P450 17‐hydroxylase/C17–20 lyase (CYP17) and cytochrome P450 aromatase (CYP19) using quantitative real‐time RT‐PCR and the localization of CYP17 mRNA in tissues by in situ hybridization. The CYP17 mRNA levels in males at 4–11 weeks postmetamorphosis were higher than in female and intersex gonads. This corresponded to their localization in the gonadal tissues, where CYP17 signals were specifically detected in the Leydig cells of the testis at 5–16 weeks postmetamorphosis but was undetectable in all ovary samples. The CYP19 mRNA levels in females at 4–11 weeks postmetamorphosis was higher than in male and intersex gonads, which corresponded with gonadal development, indicating the potential steroidogenic function of the ovary. Based on the present results, the role of CYP17 and CYP19 mRNA in sex differentiation in H. rugulosus may occur after gonadal sex differentiation and the steroidogenic potential of the gonads exhibited a sexual dimorphic pattern. These results provide a crucial basis for further research on the developmental biology in anuran species.

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Section: Discussionmentioning

confidence: 99%

Steroidogenic potential of the gonad during sex differentiation in the rice field frog Hoplobatrachus rugulosus (Anura: Dicroglossidae)

et al. 2023

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“…The expression of CYP17 mRNA have been observed in several nonmammalian vertebrate species. In spotted sea bass Lateolabrax maculatus, CYP17 mRNA presented in steroidogenic tissues and the strong signals were largely found in the area of Leydig cells of the testis when observed by in situ hybridization (Chi et al, 2019). In zebrafish, CYP17A1 mRNA is prominently expressed in male gonad (Hinfray et al, 2011).…”

Section: Cloning Of Cyp17 Cyp19 and ß-Actin Cdnamentioning

confidence: 96%

Gonadal sex differentiation of rice field frog hoplobatrachus rugulosus (wiegmann, 1834)

Traijitt¹

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The rice field frog Hoplobatrachus rugulosus, a species widely distributed in Thailand, has potential to be a model in many fields of research. A preliminary study demonstrated that the gonadal differentiation in this frog showed tendency to be an undifferentiated type since all individuals had ovaries at complete metamorphosis. However, the information was still unclear. Therefore, this study aimed to investigate the chronology and pattern of gonadal sex differentiation as well as steroidogenic potential of gonadal tissues during development. The frog embryos were obtained by stimulating fertilization in laboratory under natural light and temperature conditions. Their developmental stage was identified using Gosner (1960) system. The time period during each stage of development was also recorded. H. rugulosus spent 25-33 days for development to complete metamorphosis. The gonad was firstly observed by stereomicroscope at Gosner stage 33 (9-12 days post hatch, dph), while the testis and ovary were distinguishable by morphology from 3–4 weeks after metamorphosis. Histological analysis showed that genital ridge formation began at stage 25 (1-2 dph) and ovarian differentiation began at stage 36 (13-17 dph). The developing ovary appeared with numerous primary oogonia which developed into oocytes, while the medulla regressed to form an ovarian cavity. During metamorphosis, only an ovary was observed. Testicular differentiation began later, during the first week after metamorphosis, and occurred via an intersex condition. The intersex gonads contained developing testicular tissue with both normal and atretic oocytes. The fully developed testis was firstly identified at 6 weeks after metamorphosis. Thus, the pattern of gonadal sex differentiation in H. rugulosus is an undifferentiated type, in which only female gonads are observed during metamorphosis and intersex and male gonads are observed later. For steroidogenic potential of gonad, the expression level of mRNA encoding cytochrome P450 17-hydroxylase/C17–20 lyase (CYP17) and cytochrome P450 aromatase (CYP19) were observed by quantitative real-time RT-PCR and localization of CYP17 mRNA in the tissues was observed by in situ hybridization. The results showed that CYP17 mRNA levels in male group at 4-11 weeks after metamorphosis showed a higher level than those of other groups. This result corresponded to their localization in the gonadal tissues that the CYP17 signals were specifically detected in Leydig cells of testicular tissue in both testes and intersex gonads at 4-16 weeks after metamorphosis but undetectable in all ovary samples. The CYP19 mRNA levels in female group at 4-11 weeks after metamorphosis was higher than those of other groups which corresponded with the histological results that the number of oocytes and follicular cells were increased, indicating potential steroidogenic function of the ovary. In intersex group at 4-11 weeks after metamorphosis, the level of CYP17 mRNA was high, whereas the level of CYP19 mRNA was relatively lower. It could be suggested that the testicular tissue of intersex gonad has developed and had steroidogenic function, while the ovarian tissue of intersex gonad may not function. Based on the present results, the role of CYP17 and CYP19 mRNA in sex differentiation in H. rugulosus may occur after the gonadal sex differentiated and the steroidogenic potential of the gonads exhibited sexual dimorphic pattern. These results provide a crucial basis for further research on developmental biology in anuran species.

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Skin damage caused by scale loss modifies the intestine of chronically stressed gilthead sea bream (Sparus aurata, L.)

Mateus

Mourad

Power

2021

Developmental & Comparative Immunology

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Isolation of CYP17 I, 3β-HSD and AR Genes from Spotted Sea Bass (Lateolabrax maculatus) Testis and Their Responses to Hormones and Salinity Stimulations

Cited by 4 publications

References 31 publications

Steroidogenic potential of the gonad during sex differentiation in the rice field frog Hoplobatrachus rugulosus (Anura: Dicroglossidae)

Steroidogenic potential of the gonad during sex differentiation in the rice field frog Hoplobatrachus rugulosus (Anura: Dicroglossidae)

Gonadal sex differentiation of rice field frog hoplobatrachus rugulosus (wiegmann, 1834)

Skin damage caused by scale loss modifies the intestine of chronically stressed gilthead sea bream (Sparus aurata, L.)

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