Isolation of Coxiella burnetii by a centrifugation shell-vial assay from ticks collected in Cyprus: detection by nested polymerase chain reaction (PCR) and by PCR-restriction fragment length polymorphism analyses.

Spyridaki, Ioanna; Psaroulaki, Anna; Loukaides, Fidias; Αντωνίου, Μαρία; Hadjichristodolou, Christos; Tselentis, Yannis

doi:10.4269/ajtmh.2002.66.86

Cited by 31 publications

(23 citation statements)

References 33 publications

(38 reference statements)

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“…The reference strain Nine Mile was isolated from a tick in the USA [36]. C. burnetii is able to infect more than 40 species of ticks but their role in the transmission of the disease seems to be variable according to the country [82,89,104,150]. In France, this principally concerns wild and seldom concerns farm animals [83].…”

Section: Zoonotic Aspectmentioning

confidence: 99%

Is Q Fever an emerging or re-emerging zoonosis?

2005

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-Q fever is a zoonotic disease considered as emerging or re-emerging in many countries. It is caused by Coxiella burnetii, a bacterium developing spore-like forms that are highly resistant to the environment. The most common animal reservoirs are livestock and the main source of infection is by inhalation of contaminated aerosols. Although the culture process for Coxiella is laborious, advances on the knowledge of the life cycle of the bacterium have been made. New tools have been developed to (i) improve the diagnosis of Q fever in humans and animals, and especially animal shedders, (ii) perform epidemiological studies, and (iii) prevent the disease through the use of vaccines. This review summarizes the state of the knowledge on the bacteriology and clinical manifestations of Q fever as well as its diagnosis, epidemiology, treatment and prevention in order to understand what factors are responsible for its emergence or re-emergence. Coxiella burnetii / epidemiology / bacteriology / diagnostic / control

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Section: Zoonotic Aspectmentioning

confidence: 99%

Is Q Fever an emerging or re-emerging zoonosis?

2005

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“…For example, the soft ticks Ornithodoros (Almeida et al, 2012) and Carios (Reeves et al, 2006) were found to harbour this bacterium. Hard ticks, such as Amblyomma , Rhipicephalus (Bernasconi et al, 2002), Dermacentor (Psaroulaki et al, 2006), Ixodes (Spitalska and Kocianova, 2003), Hyalomma (Spyridaki et al, 2002) and Haemaphysalis, have also been found to be infected by Coxiella endosymbionts (MachadoFerreira et al, 2011). This indicates that Coxiella-like bacteria colonise a great variety of hosts for survival and reproduction.…”

Section: Introductionmentioning

confidence: 97%

Detection of Coxiella-like endosymbiont in Haemaphysalis tick in Thailand

Arthan

Sumrandee

Hirunkanokpun

et al. 2015

Ticks and Tick-borne Diseases

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“…Polymerase Chain Reaction (PCR) and PCR-based techniques are reported to be sensitive and specific for the detection of C. burnetii from tick samples (3,17,29,31). C. burnetii were found in Ixodes ricinus (24,29), Dermacentor reticulatus (24), D. marginatus (1,23,29), Haemaphysalis concinna (24,29), H. punctata (24), H. inermis (24), H. sulcata (27), H. longicornis (13), Rhipicephalus sanguineus (3,5,22,27), R. turanicus (3,27), Ambylomma variegatum (16), Hyalomma spp.…”

Section: Introductionmentioning

confidence: 99%

“…C. burnetii were found in Ixodes ricinus (24,29), Dermacentor reticulatus (24), D. marginatus (1,23,29), Haemaphysalis concinna (24,29), H. punctata (24), H. inermis (24), H. sulcata (27), H. longicornis (13), Rhipicephalus sanguineus (3,5,22,27), R. turanicus (3,27), Ambylomma variegatum (16), Hyalomma spp. (5,22) by using conventional (23) and PCR techniques (3,5,29,31).…”

Section: Introductionmentioning

confidence: 99%

Detection of Coxiella burnetii from ticks by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism

Çapın¹,

Emre²,

Canpolat³

et al. 2013

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:For the detection of Coxiella burnetii, a total of 2472 ticks (1446 female, 1021 male and 5 nymphs) was collected from cattle and sheep of 38 provinces of Turkey. The ticks were pooled into groups of 1-7 ticks of the same province, species and gender for DNA extraction. Following DNA extraction, the groups were examined for the presence of C. burnetii DNA by using the primers CB1 and CB2. Six groups from the province of Denizli (13 groups of total 56 ticks), and one group from the province of Ankara (53 groups of total 160 ticks) were found to be positive for C. burnetii. The species of Rhipicephalus turanicus, Rhipicephalus bursa and Hyalomma excavatum were found to be infected with C. burnetii. The gender was not seem to have a role in transmission of the agent. The specificities of the PCR products were evaluated by the restriction fragment length polimorphism (RFLP) analysis. The positive PCR products were digested with the enzyme TaqІ and four bands in order of 118, 57, 43 and 39 bp's were appeared such as seen in the positive control DNA (C. burnetii Nine Mile RSA493).

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Isolation of Coxiella burnetii by a centrifugation shell-vial assay from ticks collected in Cyprus: detection by nested polymerase chain reaction (PCR) and by PCR-restriction fragment length polymorphism analyses.

Cited by 31 publications

References 33 publications

Is Q Fever an emerging or re-emerging zoonosis?

Is Q Fever an emerging or re-emerging zoonosis?

Detection of Coxiella-like endosymbiont in Haemaphysalis tick in Thailand

Detection of Coxiella burnetii from ticks by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism

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