Isolation of Clostridium difficile from dogs with digestive disorders, including stable metronidazole-resistant strains

Orden, Cristina; Blanco, José L.; Álvarez‐Pérez, Sergio; García-Sancho, Mercedes; Rodríguez-Franco, Fernando; Sainz, Á.; Villaescusa, Alejandra; Harmanus, Céline; Kuijper, Ed J.; García, Marta E.

doi:10.1016/j.anaerobe.2016.12.008

Cited by 39 publications

(38 citation statements)

References 32 publications

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“…Metronidazole is reported to be an effective antibiotic for the treatment of CDI in dogs [2] and humans [1]. However, in vitro analyses showed metronidazole-resistant strains in faeces of dogs [19,20]. In human patients, as many as 20% of CDI cases treated with antibiotics including metronidazole was reported to recur [5] and a target for FMT.…”

Section: Discussionmentioning

confidence: 99%

Oral faecal microbiota transplantation for the treatment of Clostridium difficile-associated diarrhoea in a dog: a case report

et al. 2019

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BackgroundSuccessful clinical outcomes of faecal microbiota transplantation (FMT) for recurrent Clostridium difficile infection have been reported in humans and a marmoset. However, it has been unclear whether oral FMT was effective for the treatment of C. difficile-associated diarrhoea in dogs.Case presentationAn 8-month-old, intact male French bulldog was presented with a 4-month history of intermittent large bowel diarrhoea. Physical and clinical examinations did not identify any specific causes for diarrhoea. Real-time PCR analysis and immunochromatography detected C. difficile antigen and toxin A&B genes and proteins in a faecal sample. Based on these findings, diarrhoea in the dog was considered to be induced by C. difficile-associated colitis. The dog was treated with oral FMT, in which a faecal solution obtained from a healthy beagle was orally administered to the subject. Stool consistency and frequency and faecal blood and mucus became normal 2–3 days after oral FMT, and real-time PCR analysis and immunochromatography was negative for C. difficile antigen and toxin A&B genes and proteins. No adverse events were observed.ConclusionThe present case report demonstrated that oral FMT was an effective treatment for C. difficile-associated diarrhoea in a dog. The findings in this report provide a rationale to evaluate clinical efficacy of oral FMT for other gastrointestinal diseases in dogs.

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Section: Discussionmentioning

confidence: 99%

Oral faecal microbiota transplantation for the treatment of Clostridium difficile-associated diarrhoea in a dog: a case report

et al. 2019

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“…This includes the discovery of epidemic ribotypes of multiresistant Clostridioides (Clostridium) difficile (Nagy ) in dogs with GI disorders (Orden et al . ).…”

Section: Antibacterial Resistancementioning

confidence: 97%

“…Moreover, dogs are also considered a possible reservoir of antibiotic resistant strains potentially dangerous for human patients. This includes the discovery of epidemic ribotypes of multiresistant Clostridioides (Clostridium) difficile (Nagy 2018) in dogs with GI disorders (Orden et al 2017).…”

Section: Antibacterial Resistancementioning

confidence: 99%

Proposal for rational antibacterial use in the diagnosis and treatment of dogs with chronic diarrhoea

Cerquetella

Rossi

Suchodolski

et al. 2020

J of Small Animal Practice

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Chronic diarrhoea is a frequent complaint in canine practice and the diagnostic path is often characterised by numerous diagnostic tests and stepwise empirical treatments, often applied before gastrointestinal endoscopy/mucosal biopsies. These include dietary interventions (novel protein, hydrolysed protein diet), parasiticides and still, in many cases, antibacterials. Indiscriminate use of antibacterial drugs risks detrimental consequences for both the individual patient (antimicrobial resistance, long‐term disruption of intestinal bacterial populations, potential worsening of gastrointestinal signs) and the general public. For that reason, in this Perspective essay we advocate use of antibacterials only after histopathologic evaluation of gastrointestinal biopsies or, for those cases in which endoscopy is not possible, after other therapeutic trials, such as diet/pre‐probiotics or anti‐inflammatory drugs have proven unsuccessful. They should be reserved, after appropriate dietary trials, for those canine chronic diarrhoeic patients with signs of true primary infection (i.e. signs of systemic inflammatory response syndrome or evidence of adherent‐invasive bacteria) that justify antibacterial use.

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“…, Orden et al . ). Metronidazole is currently first choice in humans for treating mild to moderate C. difficile (Spigaglia ) and therefore to reduce the risk of further resistant strains emerging it is advisable that metronidazole should only be administered in veterinary patients where there is a clear benefit.…”

Section: Discussionmentioning

confidence: 97%

“…It was deemed important to examine the need for metronidazole because resistance to this antibiotic is increasing in bacteria found in humans and veterinary species (Spigaglia 2016). Moreover metronidazole resistance is specifically reported in dogs, both to C. difficile and C. perfringens (Gobeli et al 2012, Spigaglia et al 2015, Orden et al 2017. Metronidazole is currently first choice in humans for treating mild to moderate C. difficile (Spigaglia 2016) and therefore to reduce the risk of further resistant strains emerging it is advisable that metronidazole should only be administered in veterinary patients where there is a clear benefit.…”

Section: Discussionmentioning

confidence: 99%

Evaluating the effect of metronidazole plus amoxicillin‐clavulanate versus amoxicillin‐clavulanate alone in canine haemorrhagic diarrhoea: a randomised controlled trial in primary care practice

Ortíz

Klein

Channell

et al. 2018

J of Small Animal Practice

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Isolation of Clostridium difficile from dogs with digestive disorders, including stable metronidazole-resistant strains

Cited by 39 publications

References 32 publications

Oral faecal microbiota transplantation for the treatment of Clostridium difficile-associated diarrhoea in a dog: a case report

Oral faecal microbiota transplantation for the treatment of Clostridium difficile-associated diarrhoea in a dog: a case report

Proposal for rational antibacterial use in the diagnosis and treatment of dogs with chronic diarrhoea

Evaluating the effect of metronidazole plus amoxicillin‐clavulanate versus amoxicillin‐clavulanate alone in canine haemorrhagic diarrhoea: a randomised controlled trial in primary care practice

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