Isolation of Cellulose-decomposing Fungi from the Soil

Eggins, H. O. W.; Pugh, G.J.F.

doi:10.1038/193094a0

Cited by 175 publications

(45 citation statements)

References 6 publications

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“…Cellulose hydrolysis was tested on LB agar containing 250 ml4% (w/v) suspension of ball-milled cellulose 1-l (Eggins & Pugh, 1962). A positive result was a clear zone around a streak inoculation within 21 d.…”

Section: Pen Icillium Characterization Methods 1889mentioning

confidence: 99%

An Evaluation of Some Physiological and Biochemical Methods as an Aid to the Characterization of Species of Penicillium Subsection Fasciculata

Bridge

1985

Microbiology

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Physiological and biochemical test methods have been evaluated for use in the taxonomy of filamentous microfungi, with specific reference to isolates of Penicillium subsection Fasciculata. Physiological and enzymic tests such as growth in the presence of inhibitory substances, pH limits of growth and the hydrolysis of a variety of substrates proved to be relatively easy to perform and showed promise as differential characters. Carbon and nitrogen source assimilations were technically difficult to perform and few substrates gave differential results. In the few cases where assimilation tests were successful they often involved substrates that may otherwise be inhibitory, such as organic acids and sodium nitrite. Results from assimilation tests appeared to be differential at around species level and positive results were often associated with a significant rise in growth medium pH. Many of the tests described have not previously been applied to filamentous fungi and may have applications in the study of other genera.

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Section: Pen Icillium Characterization Methods 1889mentioning

confidence: 99%

An Evaluation of Some Physiological and Biochemical Methods as an Aid to the Characterization of Species of Penicillium Subsection Fasciculata

Bridge

1985

Microbiology

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“…The following liquid media were used for submerged cultures: medium I had the same composition as that of Eggins & Pugh (1962) but without asparagine and with the addition of trace elements (10 ml 1-1 of the SL6 solution of Pfennig & Lippert, 1966), CaC12.2H20 (100 mg 1-l) and iron(II1) ammonium citrate (10 mg 1-l); medium I1 had the same composition as medium I, but (NH&S04 was replaced by an equivalent amount of Difco Casamino acids. Organic substrates were sterilized separately and mixed aseptically with the medium to give a final concentration ranging from 0.01 to 1 % (w/v) (see individual experiments).…”

Section: E T H O D Smentioning

confidence: 99%

Induction and Catabolite Repression of Cellulase Synthesis in the Thermophilic Fungus Sporotrichum thermophile

Canevascini

Coudray

Southgate

et al. 1979

Journal of General Microbiology

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A strain of Sporotrichum thermophile (var. 2), grown in submerged culture at 44 "C, decayed crystalline cellulose by the secretion of cellulolytic enzymes into the culture medium.Dialysed culture filtrates, after growth on cellobiose, wese also effective in degrading crystalline cellulose (Avicel). Cellulase, measured viscometrically with carboxymethylcellulose as substrate and therefore referred to as C M-cellulase, was induced by cellulose, glucomannan, cellobiose and, to a limited extent, by laminaribiose and cellobionic acid. Low CM-cellulase activities were also detectable when the organism was grown on other carbon sources. In culture media supplemented with readily metabolized non-inducing substrates, such as glucose, CM-cellulase activity did not increase after their exhaustion indicating that no derepression of constitutive CM-cellulase occurred. CM-cellulase synthesis induced during growth on cellobiose was strongly inhibited, although not completely suppressed, by glucose and other carbon sources. In cultures grown on glucose and cellobiose, glucose was utilized first and CM-cellulase synthesis was repressed ; cellobiose utilization occurred only after glucose exhaustion and triggered CM-cellulase formation. When glucose was added to a culture growing on cellobiose, further utilization of the latter was prevented and CM-cellulase synthesis was inhibited. The use of glucose analogues gave some indications that cellobiose did not compete with glucose for the same transport carrier and that glucose catabolism was a prerequisite for the inhibition of CM-cellulase synthesis.

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“…Suspensions of approximately 1.2 mg of peat in Ringer solution were overpoured with molten, cooled cellulose agar (Eggins and Pugh, 1962), incubated at approximately 22° C and examined at intervals up to 96 days. Fungi and Streptomycetes were recorded on these plates, on a presence or absence only basis.…”

Section: Methodsmentioning

confidence: 99%

Microbial Activity in Florida Everglades Peat

1974

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SUMMARYPopulations of viable bacteria and fungi were shown to exist in various strata of peat formed in the Florida Everglades. The populations present were quantitatively and qualitatively different in three distinct peat-forming habitats and at various depths in the peat profiles. Relatively few pieces of peat taken from horizons below the surface were colonized by actively growing fungi or actinomycetes. Direct observation of peat confirmed some features of the cultural studies. Bacteria were mostly present in small groups of two to six cells and most of the hyphae recorded were apparently formed by Actinomycetales rather than the fungi. The significance of these data in relation to possible long-term changes in the peats is discussed.

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Isolation of Cellulose-decomposing Fungi from the Soil

Cited by 175 publications

References 6 publications

An Evaluation of Some Physiological and Biochemical Methods as an Aid to the Characterization of Species of Penicillium Subsection Fasciculata

An Evaluation of Some Physiological and Biochemical Methods as an Aid to the Characterization of Species of Penicillium Subsection Fasciculata

Induction and Catabolite Repression of Cellulase Synthesis in the Thermophilic Fungus Sporotrichum thermophile

Microbial Activity in Florida Everglades Peat

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