Isolation of cellulolytic bacteria from the gastro-intestinal tract of Achatina fulica (Gastropoda: Pulmonata) and their evaluation for cellulose biodegradation

Dar, Mudasir A.; Pawar, Kiran D.; Jadhav, Jyoti P.; Pandit, Radhakrishna S.

doi:10.1016/j.ibiod.2014.11.016

Cited by 48 publications

(28 citation statements)

References 29 publications

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“…The final weight of the GS after 20 days of bacterial treatment was reduced from 1000 ± 04 mg to 670 ± 10 mg indicating 33% degradation which is quite significant for such a short duration. However, the degradation capacity of B. tequilensis G9 is much higher than our previously reported isolates [17].…”

Section: Analysis Of Hydrolyzed Gscontrasting

confidence: 72%

“…The pretreated GS was used as the sole source of carbon to induce the cellulase production by B. tequilensis G9. The media used in the study was Berg Minimal Salt (BMS) medium comprising CaCl 2 •2H 2 O, 0.5 g; FeSO 4 •7H 2 O, 0.02 g; K 2 HPO 4 , 0.5 g; MgSO 4 •7H 2 O, 0.02 g; MnSO 4 •7H 2 O, 0.02 g; NaNO 3 , 2 g per 1000 ml of solution [17]. The media and reagents were autoclaved at 121 °C for 15 min before use.…”

Section: Microorganisms and Pretreatment Of The Substratementioning

confidence: 99%

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Statistical optimization of lignocellulosic waste containing culture medium for enhanced production of cellulase by Bacillus tequilensis G9

Dar

Pawar

Chintalchere

et al. 2019

Waste Dispos. Sustain. Energy

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The ever increasing energy demands of modern civilization and rapidly dwindling fossil fuels point towards a renewable substitute like biofuels. However, higher costs associated with biofuel productions is the major bottleneck for its commercialization. The present study demonstrates the use of a statistical approach called response surface methodology (RSM) to investigate the optimum parameters for maximum production of cellulase by Bacillus tequilensis G9. The Plackett-Burman design (PB) of the RSM analysis indicated grass straw (GS) concentration, pH, FeSO 4 , inoculum, MgSO4, incubation period and NH 4 Cl as significant variables that influence the cellulase production. Further, to propose the best medium for the maximum production of cellulase by B. tequilensis G9, the most influential parameters, namely concentrations of GS as substrate, FeSO 4 , pH, inoculum size, etc. were fine-tuned by central composite design (CCD) involving four factors and five levels. The CCD analysis demonstrated 8% substrate concentration, 1.5% of inoculum along with 10 ppm FeSO 4 and a pH of 5.5 in media as optimum conditions for highest enzyme production. The field emission scanning electron microscopic analysis of the treated GS showed structural alterations depicting significant deconstruction caused by B. tequilensis G9. The yield of the partially purified cellulase proteins were found to be 21% revealing molecular mass between 30 and 97 kDa. The enhanced cellulase production by B. tequilensis G9 demonstrated in our study brands its applications in many industrial processes like biorefinery, biofuels, etc.

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Section: Analysis Of Hydrolyzed Gscontrasting

confidence: 72%

Section: Microorganisms and Pretreatment Of The Substratementioning

confidence: 99%

Statistical optimization of lignocellulosic waste containing culture medium for enhanced production of cellulase by Bacillus tequilensis G9

Dar

Pawar

Chintalchere

et al. 2019

Waste Dispos. Sustain. Energy

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“…Microorganisms isolated from nature and the guts of herbivorous animals can degrade different feedstocks. Larger animals lack the capacity to degrade lignocellulosic materials themselves and instead rely on their gut microbial communities [12]. …”

Section: Introductionmentioning

confidence: 99%

Evaluation of gastrointestinal bacterial population for the production of holocellulose enzymes for biomass deconstruction

Asem¹,

Leo²,

Passari³

et al. 2017

PLoS ONE

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The gastrointestinal (GI) habitat of ruminant and non-ruminant animals sustains a vast ensemble of microbes that are capable of utilizing lignocellulosic plant biomass. In this study, an indigenous swine (Zovawk) and a domesticated goat (Black Bengal) were investigated to isolate bacteria having plant biomass degrading enzymes. After screening and enzymatic quantification of eighty-one obtained bacterial isolates, Serratia rubidaea strain DBT4 and Aneurinibacillus aneurinilyticus strain DBT87 were revealed as the most potent strains, showing both cellulase and xylanase production. A biomass utilization study showed that submerged fermentation (SmF) of D2 (alkaline pretreated pulpy biomass) using strain DBT4 resulted in the most efficient biomass deconstruction with maximum xylanase (11.98 U/mL) and FPase (0.5 U/mL) activities (55°C, pH 8). The present study demonstrated that bacterial strains residing in the gastrointestinal region of non-ruminant swine are a promising source for lignocellulose degrading microorganisms that could be used for biomass conversion.

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“…The symbiotic bacteria from the gut of gastropods are considered to participate in the digestion of carbohydrates, such as cellulose and hemicellulose comprising the major part of the plants ( Table 2). Recently, we reported the presence of lignocellulolytic bacteria in the GI tract of A. fulica [73]. However, Koch et al [71] reported that P. canaliculata can survive till 56 days on a cellulose-rich diet and concluded the existence of bacterial endoglucanases that helps the snail to utilize cellulose polymer.…”

Section: Cellulose-degrading Bacteriamentioning

confidence: 97%

“…Further, identification of the isolated gut bacteria has been done in vetigastropods of the genus Haliotis and in several other pulmonates. Among pulmonates, representatives of the genera Biomphalaria, Bulinus, Helisoma [72], Helix, Cornu [64,66] and Achatina [70,73,74] have also been studied.…”

Section: Host-symbiont Interactionsmentioning

confidence: 99%

Gut Microbiome Analysis of Snails: A Biotechnological Approach

Dar¹,

Pawar²,

Pandit³

2017

Organismal and Molecular Malacology

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Mollusks are a diverse group of animals not only at the species level but also with respect to their habitat and behavior. Gastropods comprise 80% of the mollusks with approximately 62,000 living species including snails. Over the period of time, snails have evolved into marine, freshwater and terrestrial forms with a transitional shift in their feeding habits. From prehistoric times, mollusks have established an intimate relationship with humans. These animals are used as food, medicine, offering to gods and are also responsible for economic losses in the form of agricultural pests. As most of these animals feed on plant biomass, their guts have evolved to digest such lignocellulosic biomass with extraordinary efficiency. The plant fiber digestion in their guts depends predominantly on the metabolic activities of the gastro-intestinal microflora. Besides digestive functions, the seasonal dynamic and spatial distribution of bacterial gut community largely influences cold hardiness and many other metabolic properties in snails. Here, we assessed an overview of the various bacterial populations dwelling in digestive tracts of snails. This chapter provides insights into the gut microbiome of various snails that can be exploited for various industrial applications such as biomass degradation, production of biofuel, paper, wine and laundry detergents.

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Isolation of cellulolytic bacteria from the gastro-intestinal tract of Achatina fulica (Gastropoda: Pulmonata) and their evaluation for cellulose biodegradation

Cited by 48 publications

References 29 publications

Statistical optimization of lignocellulosic waste containing culture medium for enhanced production of cellulase by Bacillus tequilensis G9

Statistical optimization of lignocellulosic waste containing culture medium for enhanced production of cellulase by Bacillus tequilensis G9

Evaluation of gastrointestinal bacterial population for the production of holocellulose enzymes for biomass deconstruction

Gut Microbiome Analysis of Snails: A Biotechnological Approach

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