Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and Methods

Bryzgunova, Olga E.; Konoshenko, Maria; Zaporozhchenko, Ivan A.; Yakovlev, Alexey; Laktionov, Pavel P.

doi:10.3390/diagnostics11050865

Cited by 31 publications

(27 citation statements)

References 165 publications

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“…For this reason, various sample extraction kits and methods have been developed, and some of these have been reported to reduce intralaboratory and interlaboratory variability. Several research groups have presented and compared commercial RNA extraction kits, and studies related to the standardization of miRNA extraction methods from biological fluids have been conducted [ 46 , 47 , 48 ]. Column-based extraction kits often obtain high-quality miRNA extracts, and they are associated with lower extraction variation chloroform–phenol-based kits.…”

Section: Factors Inhibiting Accurate Mirna Measurement In Plasma Usin...mentioning

confidence: 99%

Considerations and Suggestions for the Reliable Analysis of miRNA in Plasma Using qRT-PCR

Ban

Song

2022

Genes

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MicroRNAs (miRNAs) are promising molecules that can regulate gene expression, and their expression level and type have been associated with early diagnosis, targeted therapy, and prognosis of various diseases. Therefore, analysis of miRNA in the plasma or serum is useful for the discovery of biomarkers and the diagnosis of implicated diseases to achieve potentially unprecedented progress in early treatment. Numerous methods to improve sensitivity have recently been proposed and confirmed to be valuable in miRNA detection. Specifically, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) is an effective and common method for sensitive and specific analysis of miRNA from biological fluids, such as plasma or serum. Despite this, the application of qRT-PCR is limited, as it can be affected by various contaminants. Therefore, extraction studies have been frequently conducted to maximize the extracted miRNA amount while simultaneously minimizing contaminants. Moreover, studies have evaluated extraction efficiency and normalization of the extracted sample. However, variability in results among laboratories still exists. In this review, we aimed to summarize the factors influencing the qualification and quantification of miRNAs in the plasma using qRT-PCR. Factors influencing reliable analysis of miRNA using qRT-PCR are described in detail. Additionally, we aimed to describe the importance of evaluating extraction and normalization for reliable miRNA analysis and to explore how miRNA detection accuracy, especially from plasma, can be improved.

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Section: Factors Inhibiting Accurate Mirna Measurement In Plasma Usin...mentioning

confidence: 99%

Considerations and Suggestions for the Reliable Analysis of miRNA in Plasma Using qRT-PCR

Ban

Song

2022

Genes

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“…Cell-free miRNAs (cfmiRNAs) can be released from different tumor areas or tumor nodes, and, therefore, a cfmiRNA profile reflects a patient’s generalized tumor phenotype. Considering the well-developed protocols for cfmiRNA isolation and evaluation of their sets and concentrations, cfmiRNAs could be promising diagnostic markers [ 16 ]. The availability of liquid media, such as blood, sputum, and saliva, and methods that do not require invasive procedures have provided an opportunity for using liquid biopsies in the diagnosis of cancers, including LC [ 17 ].…”

Section: Micrornas and Lung Cancer Chemotherapymentioning

confidence: 99%

MicroRNAs as Predictors of Lung-Cancer Resistance and Sensitivity to Cisplatin

Konoshenko

Lansukhay

Красильников

et al. 2022

IJMS

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Background: Platinum-based chemotherapy, cisplatin (DDP) specifically, is the main strategy for treating lung cancer (LC). However, currently, there is a lack of predictive drug-resistance markers, and there is increased interest in the development of a reliable and sensitive panels of markers for DDP chemotherapy-effectiveness prediction. MicroRNAs represent a perspective pool of markers for chemotherapy effectiveness. Objectives: Data on miRNAs associated with LC DDP chemotherapy response are summarized and analyzed. Materials and methods: A comprehensive review of the data in the literature and an analysis of bioinformatics resources were performed. The gene targets of miRNAs, as well as their reciprocal relationships with miRNAs, were studied using several databases. Results and Discussion: The complex analysis of bioinformatics resources and the literature indicated that the expressions of 12 miRNAs have a high predictive potential for LC DDP chemotherapy responses. The obtained information was discussed from the point of view of the main mechanisms of LC chemoresistance. Conclusions: An overview of the published data and bioinformatics resources, with respect to the predictive microRNA markers of chemotherapy response, is presented in this review. The selected microRNAs and gene panel have a high potential for predicting LC DDP sensitiveness or DDP resistance as well as for the development of a DDP co-therapy.

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“…Since cf-RNA is a source of multiple RNA types as described above, some, such as cf-miRNA, are more stable than others. Cf-miRNA interacts with other biomolecules or packaging membrane-coated or membrane-free particles which protect them from the RNases present in the circulation [43].…”

Section: Technologies In Rna Isolation and Detectionmentioning

confidence: 99%

“…The review from Bryzgunova et al, 2021 outlines the variety of methods of cf-miRNA isolation from different body fluids [43]. As in blood analysis, a centrifugation step is crucial to eliminate any cell debris that can interact with the RNA fraction.…”

Section: Other Body Fluidsmentioning

confidence: 99%

Current Technologies for RNA-Directed Liquid Diagnostics

Martinez-Dominguez

Zottel

Šamec

et al. 2021

Cancers

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There is unequivocal acceptance of the variety of enormous potential liquid nucleic acid-based diagnostics seems to offer. However, the existing controversies and the increased awareness of RNA-based techniques in society during the current global COVID-19 pandemic have made the readiness of liquid nucleic acid-based diagnostics for routine use a matter of concern. In this regard—and in the context of oncology—our review presented and discussed the status quo of RNA-based liquid diagnostics. We summarized the technical background of the available assays and benchmarked their applicability against each other. Herein, we compared the technology readiness level in the clinical context, economic aspects, implementation as part of routine point-of-care testing as well as performance power. Since the preventive care market is the most promising application sector, we also investigated whether the developments predominantly occur in the context of early disease detection or surveillance of therapy success. In addition, we provided a careful view on the current biotechnology investment activities in this sector to indicate the most attractive strategies for future economic success. Taken together, our review shall serve as a current reference, at the interplay of technology, clinical use and economic potential, to guide the interested readers in this rapid developing sector of precision medicine.

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Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and Methods

Cited by 31 publications

References 165 publications

Considerations and Suggestions for the Reliable Analysis of miRNA in Plasma Using qRT-PCR

Considerations and Suggestions for the Reliable Analysis of miRNA in Plasma Using qRT-PCR

MicroRNAs as Predictors of Lung-Cancer Resistance and Sensitivity to Cisplatin

Current Technologies for RNA-Directed Liquid Diagnostics

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