Isolation of cDNA sequences encoding the MAP kinase HOG1 and the MAP kinase kinase PBS2 genes of the fungus Alternaria tenuissima through a genetic approach

Fan, Feng; Qiu, Dewen; Jiang, Lijing

doi:10.1016/j.mimet.2006.12.021

Cited by 7 publications

(4 citation statements)

References 37 publications

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“…A cotransformation was performed by using pAN7-1 plasmid, carrying the hph gene as selectable marker and conferring resistance to hygromycin B (Punt et al, 1987) and the plasmid pGEMT-easy (Promega, Madison, USA) with an inserted 600 bp sequence of aahog. This 600 bp sequence is an internal fragment of the 1539 bp complete cDNA sequence AtHOG1, the hog1 homologue of A. tenuissima published by Feng et al (2007). To amplify the 600 bp sequence of A. alternata the following primer pairs were deduced from the A. tenuissima sequence: HOG1_A7-for (5′-GATTGCCGGACCTAGC-3′) and HOG1_A7-rev (5′-AATACGTCCACTCCGCT-3′).…”

Section: Protoplast Transformationmentioning

confidence: 99%

HOG MAP kinase regulation of alternariol biosynthesis in Alternaria alternata is important for substrate colonization

Graf

Schmidt‐Heydt

Geisen

2012

International Journal of Food Microbiology

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Section: Protoplast Transformationmentioning

confidence: 99%

HOG MAP kinase regulation of alternariol biosynthesis in Alternaria alternata is important for substrate colonization

Graf

Schmidt‐Heydt

Geisen

2012

International Journal of Food Microbiology

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“…8 It is found widespread in the environment. 9 Previous chemical investigations of A. tenuissima have focused on the isolation and characterization of its toxic metabolites alternariol, 12 alternariol monomethyl ether, 12 altenuene, 12 tenuazonic acid, 13 caseinolytic protease enzyme, 12 and beta-1,3-glucanases. 13 Many Alternaria sp.…”

Section: Introductionmentioning

confidence: 99%

Altertoxins with potent anti-HIV activity from Alternaria tenuissima QUE1Se, a fungal endophyte of Quercus emoryi

Bashyal

Wellensiek

Ramakrishnan

et al. 2014

Bioorganic & Medicinal Chemistry

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Screening of a small library of natural product extracts derived from endophytic fungi of the Sonoran desert plants in a cell-based anti-HIV assay involving T-cells infected with the HIV-1 virus identified the EtOAc extract of a fermentation broth of Alternaria tenuissima QUE1Se inhabiting the stem tissue of Quercus emoryi as a promising candidate for further investigation. Bioactivity-guided fractionation of this extract led to the isolation and identification of two new metabolites, altertoxins V (1) and VI (2) together with the known compounds, altertoxins I (3), II (4), and III (5). The structures of 1 and 2 were determined by detailed spectroscopic analysis and those of 3–5 were established by comparison with reported data. When tested in our cell-based assay at concentrations insignificantly toxic to T-cells, altertoxins V (1), I (3), II (4), and III (5) completely inhibited replication of the HIV-1 virus at concentrations of 0.50, 2.20, 0.30, and 1.50 μM respectively. Our findings suggest that the epoxyperylene structural scaffold in altertoxins may be manipulated to produce potent anti-HIV therapeutics. 2014 Elsevier Ltd. All rights reserved.

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“…Despite being a plant and human pathogen, A. tenuissima is a cosmopolitan and seasonal fungus that has developed a highly adjustable mechanism to different environmental stresses (Feng et al, 2007;Peever et al, 2004;Rashman et al, 2002). To date, A. tenuissima is known to infect various parts of economically important plants belonging to different families and in different parts of the world (Mirkova and Konstantinova, 2003;Peever et al, 2004;Gannibal et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…According to Anderson et al (2002), Gannibal and Klemsdal (2007) and Rotem (1994), it can infect cereal grains and be a source of food contamination. In humans, it appears to be a cutaneous opportunistic pathogen associated with the increasing use of immuno-suppressants, immunological impairment due to organ transplants or neoplastic diseases (Feng et al, 2007). Its pathogenicity is mainly associated with the production of melanin and host specific toxin (Kishore et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Co-infection of Tylosema esculentum (Marama bean) seed pods by Alternaria tenuissima and a Phoma spp.

Uzabakiriho¹,

C.J²,

Shikongo³

et al. 2013

Afr. J. Biotechnol.

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Marama bean, Tylosema esculentum, is an endemic, perennial wild tuberous Fabaceae, widely distributed in the Southern Kalahari (Namibia, Botswana and to a lesser extends in South Africa). It is well adapted to the deep sandy soils of the Kalahari. It is drought avoiding. It has been valued by the indigenous people of the semi arid land of the Kalahari for its nutritional and medicinal qualities. The aim of the study was to determine the identity of fungal pathogens that occur on marama bean. Diseased pods presenting necrotic spots with dense sporulation in the centre were collected. They were subjected to fungal isolation using potato dextrose agar (PDA) at room temperature under fluorescent light for five days. Two fungi with different cultural conidial morphology were isolated. Single spores from the two cultures were separately inoculated on PDA to obtain a pure culture. Genomic fungal DNA was extracted from fresh mycelium using the cetyl trimethylammonium bromide (CTAB) method and was used as a template in internal transcribed spacer (ITS) PCR amplification. Based on the sequence analysis of the 5.8S ribosomal DNA and the ITS, the two isolates associated with necrotic Marama pods were identified as Alternaria tenuissima and Phoma spp. Isolate Po72 due to the high sequence homology, which was 99% in both cases. To our knowledge, this is the first report describing the presence of these two fungi on Marama bean.

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Isolation of cDNA sequences encoding the MAP kinase HOG1 and the MAP kinase kinase PBS2 genes of the fungus Alternaria tenuissima through a genetic approach

Cited by 7 publications

References 37 publications

HOG MAP kinase regulation of alternariol biosynthesis in Alternaria alternata is important for substrate colonization

HOG MAP kinase regulation of alternariol biosynthesis in Alternaria alternata is important for substrate colonization

Altertoxins with potent anti-HIV activity from Alternaria tenuissima QUE1Se, a fungal endophyte of Quercus emoryi

Co-infection of Tylosema esculentum (Marama bean) seed pods by Alternaria tenuissima and a Phoma spp.

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