Isolation of cathepsin B inhibitory peptides, Cabin-A1 and -A2, from a tryptic and chymotryptic hydrolysate of human serum albumin

Nakagomi, Kazuya; Takatsu, Kouki; Takagi, Shinobu; Ebisu, Hidetoshi; Sadakane, Yutaka; Fujii, Nobutaka; Akizawa, Toshifumi; Tanimura, Takenori; Hatanaka, Y.

doi:10.1016/s0196-9781(02)00098-0

Cited by 5 publications

(5 citation statements)

References 23 publications

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“…Tryptic and chymotryptic digestion of HSA generated two peptides with cathepsin B inhibitory properties. These peptides corresponded to fragments 65-70 (SLHTLF; one letter code) and 403-407 (FQNAL) of HSA and named cabin-A1 and cabin-A2, respectively (Nakagomi et al 2002). The trypsin digest of HSA originated peptides with angiotensin converting enzyme (ACE) inhibition activity too.…”

Section: Serum Albumin Cryptidesmentioning

confidence: 99%

Cryptides: latent peptides everywhere

Iavarone

Desiderio

Vitali

et al. 2018

Critical Reviews in Biochemistry and Molecular Biology

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Proteomic surveys with top-down platforms are today revealing thousands of naturally occurring fragments of bigger proteins. Some of them have not functional meaning because they derive from pathways responsible for protein degradation, but many have specific functions, often completely different from that one of the parent proteins. These peptides encrypted in the protein sequence are nowadays called cryptides. They are frequent in the animal and plant kingdoms and represent a new interesting -omic field of investigation. To point out how much widespread is their presence, we describe here the most studied cryptides from very common sources such as serum albumin, immunoglobulins, hemoglobin, and from saliva and milk proteins. Given its vastness, it is unfeasible to cover the topic exhaustively, therefore only several selected examples of cryptides from other sources are thereafter reported. Demanding is the development of new -omic platforms for the functional screening of new cryptides, which could provide suggestion for peptides and peptido-mimetics with variegate fields of application.

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Section: Serum Albumin Cryptidesmentioning

confidence: 99%

Cryptides: latent peptides everywhere

Iavarone

Desiderio

Vitali

et al. 2018

Critical Reviews in Biochemistry and Molecular Biology

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“…Recently, the bioactivity of HA was studied using either the synthetic peptide or the HA purified by RP-HPLC from a 3-h tryptic hydrolysate of HSA at pH 8.4 (Nakagomi et al, 2002). The procedure for HA purification implies two successive chromatographic steps, after which, the fraction eluted from the HPLC column contains three different peptides, including HA.…”

Section: Specific Release Of Human Albutensin From Hsamentioning

confidence: 99%

“…HA was found in tryptic hydrolysates of HSA (Takahashi et al, 1998) and it was reported to show strong competitive ACE inhibition (Nakagomi et al, 2000). In recent work (Nakagomi et al, 2002), HA was found in the tryptic hydrolysate of HSA and the HPLC-purified peptide inhibited cathepsin B in vitro. For this reason, usage of HA in the development of anti-cathepsin B drugs has been suggested (Nakagomi et al, 2002).…”

Section: Introductionmentioning

confidence: 96%

“…In recent work (Nakagomi et al, 2002), HA was found in the tryptic hydrolysate of HSA and the HPLC-purified peptide inhibited cathepsin B in vitro. For this reason, usage of HA in the development of anti-cathepsin B drugs has been suggested (Nakagomi et al, 2002). HA has also been reported to be an ileumconctracting peptide (Takahashi et al, 1998) and, recently, this peptide was found to decrease food intake in mice after central or peripheral administration (Ohinata et al, 2002).…”

Section: Introductionmentioning

confidence: 97%

“…Many peptides deriving from bovine milk protein have been studied and widely reviewed in the literature (Meisel, 2001;Meisel & Schlimme, 1990, 1996Shah, 2000;Silva & Malcata, 2005;Tirelli, De Noni, & Resmini, 1997). In contrast, few bioactive peptides have been isolated from enzymatic hydrolysates of human protein (Nakagomi et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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