Isolation of Cardenolide Glycosides from Pergularia Tomentosa.L and Their Antioxidant Activities

Babaamer, Zohra; Zarga, Musa Abu; Al-Abdallat, Nesren Ghazi

doi:10.12816/0039179

Cited by 3 publications

(2 citation statements)

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“…Furthermore, studies on a similar group of compounds (cardenolides) demonstrated their slight antioxidant effectiveness. However, the DPPH scavenging actions, recorded for three cardenolide glycosides isolated from Pergularia tomentosa L., were significantly lower (EC 50 ¼ 1953-3410 lg/mL) (Babaamer et al 2014) in comparison to results, obtained in this study on K. daigremontiana.…”

Section: Hplc Analysis Of Low-molecular Thiolscontrasting

confidence: 90%

Antioxidant efficacy of Kalanchoe daigremontiana bufadienolide-rich fraction in blood plasma in vitro

Kołodziejczyk-Czepas

Nowak

Wachowicz

et al. 2016

Pharmaceutical Biology

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Context: The main source of bufadienolides is toad venom; however, plants such as members of Kalanchoe Adans. (Crassulaceae) genus may also synthesize these bioactive substances. Objective: This is the first study on antioxidant effects and cytotoxicity of bufadienolide-rich fraction isolated from Kalanchoe daigremontiana Raym.-Hamet & H. Perrier. Materials and methods: The methanolic fraction was extracted from the plant roots and contained 0.48 mg bufadienolides/mg of dry mass (11a,19-dihydroksytelocinobufagin, bersaldegenin-1-acetate, bersaldegenin-1,3,5-orthoacetate, 19-(acetyloxy)-3b,5b,11a,14-tetrahydroxyl-12-oxo-bufa-20,22-dienolide and 19-(acetyloxy)-1b,3b,5b,14-tetrahydroxyl-bufa-20,22-dienolide, mainly). The cytotoxicity of K. daigremontiana fraction was evaluated in an in vitro experimental model of blood platelets. The viability of blood platelets was determined on the basis of a release of lactate dehydrogenase. Results: The fraction scavenged DPPH radicals, with EC 50 of 21.80 lg/mL. Studies on an experimental model of blood plasma under peroxynitrite-induced oxidative stress revealed that the plant preparation had moderate antioxidant properties. Levels of 3-nitrotyrosine and thiol groups indicated that the protective effect of K. daigremontiana was significant mainly for its concentration of 50 lg/mL. No effect was found in prevention of oxidation of low-molecular plasma thiols (glutathione, cysteine and cysteinylglycine). Simultaneously, measurements of lipid hydroperoxides and thiobarbituric acid-reactive substances (TBARS) indicated that the examined fraction might be effective antioxidant at broader concentration range, that is 1-5 and 25-50 lg/mL for hydroperoxides and TBARS generation, respectively. No cytotoxicity was observed at the concentration range of 1-50 lg/mL. Conclusions: Based on the obtained results, we suggest that antioxidant activity may additionally contribute to beneficial properties of K. daigremontiana-derived extracts.ARTICLE HISTORY

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Section: Hplc Analysis Of Low-molecular Thiolscontrasting

confidence: 90%

Antioxidant efficacy of Kalanchoe daigremontiana bufadienolide-rich fraction in blood plasma in vitro

Kołodziejczyk-Czepas

Nowak

Wachowicz

et al. 2016

Pharmaceutical Biology

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“…IC50 value (the concentration of extract in µg/ml required to scavenge 50% of the DPPH free radical) was calculated from the inhibition curve using the percentage scavenging activities or percentage inhibition at ve different concentrations of the compound. [20,21] The crude extract was tested for antimicrobial activity using agar disk diffusion methods as follows: A lter paper disk (6 mm) was impregnated with 10 µg of the crude extract, dried and laid on surface of nutrient agar plates inoculated with 0.1µl test microorganisms (S. aureus, Methicillin resistant S.aureus (MRSA), and E.coli The plates were left at 4°C for 12-16 hours then incubated overnight at 37°C. Inhibition zone of test microorganisms around the paper disks were measured.…”

Section: Antioxidant Activity [18 19]mentioning

confidence: 99%

Cardenolide glycosides from the aerial parts of Pergularia tomentosa.L and their biological activities

Babaamer¹,

Al-abdallah²,

kouadri³

et al. 2023

Preprint

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The chemical constituents from the aerial parts of P. tomentosa.L. were studied. The constituents were isolated and purified by various chromatography methods, and the spectroscopic methods (MS, NMR) were used to identify their structures. A new cardenolide glycoside 1, along with five known cardenolide glycosides calactin (2), 12 β-hydroxycalotropin (3), 6’-hydroxycalactin (4), galakinoside (5) and uscharidin (6) were identified and isolated for the first time from Pergularia tomentosa. L. In addition, antioxidant activities of the cardenolides were studied, the highest scavenging activity of DPPH was observed in (1) (IC50 = 2050 µg/ ml), and (2) (IC50 = 2050 µg/ ml) followed by (3) (IC50 = 2178 µg/ ml), and (4) (IC50 = 3384µg/ ml). Compounds (1–6) showed no activity against the bacterial strains S. aureus bacteria (MRSA), S. aureus, and E.coli.

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Bioactivities and in silico study of Pergularia tomentosa L. phytochemicals as potent antimicrobial agents targeting type IIA topoisomerase, TyrRS, and Sap1 virulence proteins

Haddaji

Papetti

Noumi

et al. 2021

Environ Sci Pollut Res

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Isolation of Cardenolide Glycosides from Pergularia Tomentosa.L and Their Antioxidant Activities

Cited by 3 publications

References 0 publications

Antioxidant efficacy of Kalanchoe daigremontiana bufadienolide-rich fraction in blood plasma in vitro

Antioxidant efficacy of Kalanchoe daigremontiana bufadienolide-rich fraction in blood plasma in vitro

Cardenolide glycosides from the aerial parts of Pergularia tomentosa.L and their biological activities

Bioactivities and in silico study of Pergularia tomentosa L. phytochemicals as potent antimicrobial agents targeting type IIA topoisomerase, TyrRS, and Sap1 virulence proteins

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