Isolation of Candida Species in clinical specimens and its virulence factor: The biofilm

Nerurkar, Alka; Solanky, Priti; Chavda, Nilesh; Baria, Hinal; Desai, Binita

doi:10.5455/ijmsph.2012.1.97-100

Cited by 11 publications

(11 citation statements)

References 7 publications

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“…We studied 142 Candida strains isolated from various clinical specimens, which showed significant predominance (p value <0.001) of C. albicans over non-albicans Candida species, although 36.6% strains were those of non-albicans Candida species. Similar to our findings, studies from other regions of India have also reported C. albicans as the most common isolated species with the trends towards increasing prevalence of infections caused by non-albicans Candida species [4,8,9]. However, Golia et al from Bangalore observed higher rate of isolation of non-albicans Candida species as compared to C. albicans [10].…”

Section: Discussionsupporting

confidence: 73%

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Speciation, biofilm formation and antifungal susceptibility of Candida isolates

Arora¹,

Dhuria²,

Jindal³

et al. 2017

IJRDPL

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Abstract:Recently, an increase in the incidence of infections caused by fungi especially non-albicans Candida species has been reported. Several virulence factors like biofilm formation, toxin production and presence of adhesins contribute to its pathogenesis. This study was undertaken to determine species distribution, biofilm formation and in-vitro antifungal susceptibility of Candida isolated in our tertiary care hospital. One hundred and forty-two clinical isolates obtained from various clinical specimens were subjected to KOH smear and cultured on Sabouraud's Dextrose agar medium. Conventional methods and automated identification system (Vitek 2 Compact) for yeast identification were done. Biofilm forming ability of each isolate was detected using microtitre plate method. Antifungal susceptibility against fluconazole, voriconazole, flucytosine, amphotericin B and caspofungin was tested using Vitek 2 Compact. Out of 142 Candida isolates, 90 (63.4%) were C. albicans and 52 (36.6%) were non-albicans Candida species. Among 52 nonalbicans Candida, C. parapsilosis was found in 20 (38.5%) cases followed by C. tropicalis 16 (30.8%). Among all isolates, 52 (36.6%) were biofilm producers and biofilm positivity was more among non-albicans Candida 28 (53.8%) as compared to C. albicans 24 (26.7%) (p-value <0.002). The maximum positivity was observed with isolates from plastic devices (60%). The minimum inhibitory concentrations of all isolates against antifungal drugs were within susceptible range. Although C. albicans remains the major isolate from various clinical specimens, infections caused by non-albicans. Candida is on the rise and biofilm formation as a virulence factor might have a higher significance for non-albicans Candida species than for C. albicans. The changing epidemiology of Candida infections highlights the need for close monitoring on the distribution, biofilm production and susceptibility to optimize therapy and outcome.

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Section: Discussionsupporting

confidence: 73%

“…showed that more of C. albicans isolates were biofilm producers than non-albicans species of Candida [8]. Among the non-albicans Candida species, 62.5% of C. tropicalis and 60% of C. parapsilosis were found to be biofilm producers.…”

Section: Discussionmentioning

confidence: 98%

Speciation, biofilm formation and antifungal susceptibility of Candida isolates

Arora¹,

Dhuria²,

Jindal³

et al. 2017

IJRDPL

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“…The majority of isolates (95.8%) formed biofilm. Our results found that this happened up to 82.6% in contrast with the 68.5% of Candida isolates that formed moderate to strongly [44]. It could thus be suggested that the difference between biofilm formations is related to the fact that our samples were collected from the oral cavity while the previous study deals with clinical isolates.…”

Section: Discussioncontrasting

confidence: 53%

“…In contrast, the previous studies reported that K. marxianus is an etiological agent in immunocompromised patients and that it forms a biofilm [47]. It could be concluded that species with less pathogenicity have less potency when they form biofilms [44]. It could be concluded that forming biofilm is multifactorial process that needs further analysis.…”

Section: Discussionmentioning

confidence: 79%

Innate pathogenic traits in oral yeasts

Al-laaeiby¹,

Al-Mousawi²,

Alrubayae³

et al. 2020

Karbala International Journal of Modern Science

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“…Although Kluyveromyces marxianus is not usually a pathogen for human disease, it has been recovered from catheters, associated with canditdemia, and found in biofilms on many indwelling deovices such as prosthetic heart valves and pacemakers. K. marxianus has been seen in about 1% to 3% of cases with female genital infections, surgical wounds, and infections related to upper respiratory tracts [2,3].…”

Section: Introductionmentioning

confidence: 99%

Antifungal Properties of Silver Nanoparticles Synthe‌sized From Capparis Spinosa Fruit

et al. 2019

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Background: Nanoparticles (NPs) are colloidal systems with particles ranging from 10 to 100 nm in diameter. Because of their large surface-volume ratio, NPs are biologically active materials that could interact with biomolecules and microorganisms, enter into the cells, and affect the metabolic functions. The study aimed to biosynthesize silver nanoparticles (Ag-NPs) from Capparis spinosa fruit aqueous extract, and evaluate their Ag nanostructure characterization and antifungal activity.Materials and Methods: Capparis spinosa fruit aqueous was prepared with the percolation method. Then, silver NPs were synthesized using 0.01 M silver nitrate solution, and their formation was validated by color changing of the solution from green to dark brown . The NPs were purified using centrifugation and then dried in an oven for further analyses. Ag-NPs nanostructure characterization was determined by various techniques such as Fourier Transforms Infrared (FTIR) spectroscopy, Scanning Electron Microscope (SEM), and Ultraviolet-visible (UV-Vis) spectroscopy. Antifungal activity of Ag-NPs against three pathogenic fungi of Candida albicans, Candida glabrata, and Kluyveromyces marxianus was also evaluated using the microdilution method.Results: Synthesis of Ag-NPs from aqueous extract of C. spinosa fruit was done successfully. UV-Vis spectrum of Ag-NPs showed an absorbance peak around 420 nm, revealing Ag-NPs surface plasmon resonance (K max ). FTIR analysis showed that functional groups correspond to plant bioactive components, promoting the formation of Ag-NPs. Furthermore, spherical uniformity of the synthesized Ag-NPs from plant extract was confirmed by SEM analysis within the 50-80 nm size range. Our results showed that the produced Ag-NPs were spherical and in a suitable form and size (50-80 nm). The biosynthesized Ag-NPs had an inhibitory effect against all tested fungi with the minimum inhibitory concentration of 2500, 5000, and 625 µg/mL and minimum bactericidal concentration of 10000, 10000, and 156.25 µg/mL for C. albicans, C. glabrata, and K. marxianus, respectively. Conclusion:According to the UV-Vis spectrum, FTIR, and SEM results, we succeeded in synthesizing Ag-NPs from C. spinosa fruit aqueous extract. This research was the first report of Ag-NPs synthesized from aqueous extract of C. spinosa fruit. Our simple, quick, and inexpensive method for biosynthesis of a nanoparticle, which showed antifungal activity, provides a new potential antifungal agent for therapeutic applications.

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Isolation of Candida Species in clinical specimens and its virulence factor: The biofilm

Cited by 11 publications

References 7 publications

Speciation, biofilm formation and antifungal susceptibility of Candida isolates

Speciation, biofilm formation and antifungal susceptibility of Candida isolates

Innate pathogenic traits in oral yeasts

Antifungal Properties of Silver Nanoparticles Synthe‌sized From Capparis Spinosa Fruit

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