Isolation of cancer stem cells from adult glioblastoma multiforme

Yuan, Xiangpeng; Curtin, James F.; Xiong, Yizhi; Liu, Gentao; Waschsmann-Hogiu, Sebastian; Farkas, Daniel L.; Black, Keith L.; Yu, John S.

doi:10.1038/sj.onc.1208311

Cited by 733 publications

(674 citation statements)

References 27 publications

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“…If gliomas can be accurately mapped, IMRT may provide further advantages because of its ability to target selected, more resistant parts within tumor with higher radiation doses without increasing the doses to normal tissues. ( 52 , 53 ) …”

Section: Discussionmentioning

confidence: 99%

Intensity modulated radiation therapy versus three‐dimensional conformal radiation therapy for the treatment of high grade glioma: a dosimetric comparison

MacDonald

Ahmad

Kachris

et al. 2007

J Applied Clin Med Phys

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The present study compared the dosimetry of intensity‐modulated radiation therapy (IMRT) and three‐dimensional conformal radiation therapy (3D‐CRT) techniques in patients treated for high‐grade glioma. A total of 20 patients underwent computed tomography treatment planning in conjunction with magnetic resonance imaging fusion. Prescription dose and normal‐tissue constraints were identical for the 3D‐CRT and IMRT plans. The prescribed dose was 59.4 Gy delivered at 1.8 Gy per fraction using 4 – 10 MV photons. Normal‐tissue dose constraints were 50 – 54 Gy for the optic chiasm and nerves, and 55 – 60 Gy for the brainstem.The IMRT plan yielded superior target coverage as compared with the 3D‐CRT plan. Specifically, minimum and mean planning target volume cone down doses were 54.52 Gy and 61.74 Gy for IMRT and 50.56 Gy and 60.06 Gy for 3D‐CRT (p≤0.01). The IMRT plan reduced the percent volume of brainstem receiving a dose greater than 45 Gy by 31% (p=0.004) and the percent volume of brain receiving a dose greater than 18 Gy, 24 Gy, and 45 Gy by 10% (p=0.059), 14% (p=0.015), and 40% (p≤0.0001) respectively. With IMRT, the percent volume of optic chiasm receiving more than 45 Gy was also reduced by 30.40% (p=0.047). As compared with 3D‐CRT, IMRT significantly increased the tumor control probability (p≤0.005) and lowered the normal‐tissue complication probability for brain and brainstem (p<0.033).Intensity‐modulated radiation therapy improved target coverage and reduced radiation dose to the brain, brainstem, and optic chiasm. With the availability of new cancer imaging tools and more effective systemic agents, IMRT may be used to intensify tumor doses while minimizing toxicity, therefore potentially improving outcomes in patients with high‐grade glioma.PACs number: 87.53 Tf

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Section: Discussionmentioning

confidence: 99%

Intensity modulated radiation therapy versus three‐dimensional conformal radiation therapy for the treatment of high grade glioma: a dosimetric comparison

MacDonald

Ahmad

Kachris

et al. 2007

J Applied Clin Med Phys

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“…GSC have been shown to express Nestin, a classic neural stem cell marker and the surface marker cluster of differentiation (CD) 133 (also known as prominin 1), which are nowadays used as clinical prognosis molecules for glioma (Lendahl et al, 1990;Ignatova et al, 2002;Singh et al, 2003;Singh et al, 2004;Yuan et al, 2004;Zhang et al, 2008). However, recent results suggest that CD133 expression may not be a reliable marker for the tumorigenic ability of stem cells in the brain.…”

Section: Molecular Markers Of Glioma Stem Cellsmentioning

confidence: 99%

Neuronal stem cells in the central nervous system and in human diseases

Wang

2012

Protein Cell

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The process of cortical expansion in the central nervous system is a key step of mammalian brain development to ensure its physiological function. Radial glial (RG) cells are a glial cell type contributing to this progress as intermediate neural progenitor cells responsible for an increase in the number of cortical neurons. In this review, we discuss the current understanding of RG cells during neurogenesis and provide further information on the mechanisms of neurodevelopmental diseases and stem cell-related brain tumorigenesis. Knowledge of neuronal stem cell and relative diseases will bridge benchmark research through translational studies to clinical therapeutic treatments of these diseases.

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“…In mature adult tissues, stem cells and progenitor cells may also constitute the sources of oncogenic transformation following somatic mutations, as shown by the isolation of TSCs from human acute myeloid leukemia (AML) [3][4][5][6] and breast cancer. [7][8][9] In tumors of the central nervous system (CNS), TSCs have also been isolated and successfully maintained in culture from medulloblastoma, 10 glioblastoma, [11][12][13][14][15][16][17][18] and ependymoma, 19 as well as from several glioma cell lines. 17,20 These results suggest that brain tumors may contain a subpopulation of precursor cells that share similar surface markers and properties with neural stem cells.…”

mentioning

confidence: 99%

Isolation and characterization of stem cell-like precursor cells from primary human anaplastic oligoastrocytoma

Zhou

Ping

et al. 2007

Modern Pathology

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A small population of stem cell-like precursors in solid tumors are linked to histological composition, progression, angiogenesis, metastasis, recurrence and drug resistance of a variety of malignant tumors. Oligoastrocytoma is the most common brain mixed glioma composed of mixed cells of oligodendroglial and astrocytic phenotypes. Identification and characterization of stem cell-like precursors in oligoastrocytoma may shed light on the oncogenesis of this unique type of tumor and assist in the design of novel therapeutic strategy. Here, tumor stem cell-like precursors were identified from primary human anaplastic oligoastrocytomas by labeling of the tumor sections with nestin and CD133. Tumor cells were cultured in vitro in stem cell medium with growth factors and the capacity of the surviving stem cell-like precursors to form tumor spheres was tested. The tumor spheres were further injected subcutaneously into nude mice to observe the contribution of stem cell-like precursors to histological composition and tumor progression. We found that primary human oligoastrocytoma tissues contained nestin þ /CD133 þ stem cell-like precursors. These cells differentiated into tumor cells with both oligodendroglial and astrocytic characteristics and formed tumor spheres in vitro, which upon implantation in nude mice, grew into tumor nodules containing nestin þ /CD133 þ cells at levels higher than in the primary tumor tissues. This study revealed for the first time that anaplastic human oligoastrocytomas contained stem cell-like precursors, which exhibit neural stem cell properties with tumorigenicity. These stem cell-like precursors may be responsible for the oligodendroglial and astrocytic components of human oligoastrocytoma and should be considered as therapeutic targets.

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Isolation of cancer stem cells from adult glioblastoma multiforme

Cited by 733 publications

References 27 publications

Intensity modulated radiation therapy versus three‐dimensional conformal radiation therapy for the treatment of high grade glioma: a dosimetric comparison

Intensity modulated radiation therapy versus three‐dimensional conformal radiation therapy for the treatment of high grade glioma: a dosimetric comparison

Neuronal stem cells in the central nervous system and in human diseases

Isolation and characterization of stem cell-like precursor cells from primary human anaplastic oligoastrocytoma

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