Isolation of C Type Virus Particles From Leukemic and Lymphocytotic Cattle.

Dutta, S.; Larson, V L; Sorensen, D. K.; Perman,; Weber, Alvin F; Hammer, R. F.; Shope, R. E.

doi:10.2172/4134486

Cited by 5 publications

(6 citation statements)

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“…A definite association between bovine leukaemia virus (BLV) and lymphosarcomatous lesions of cattle was first reported by Miller et al (17) and later confirmed by other workers (7,29,30). Subsequently Miller et al (19) and several other workers established that BLV is leukaemogenic and can cause lymphosarcoma in experiment ally inoculated cattle.…”

Section: Introductionmentioning

confidence: 71%

Sero-epidemiological studies of bovine leukaemia virus infection in Indian cross-bred zebu cattle

Singh¹,

Bansal²,

Singh³

1987

Rev. Sci. Tech. OIE

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Antibodies against bovine leukaemia virus were detected in 322 of 1,511 serum samples from cattle of various age groups and both sexes, in various herds of the country. A variable degree (5.4 to 75%) of positive reactors was recorded in individual herds where no systematic breeding policy had been followed. Antibodies were also detected in clinically healthy cattle, having normal haematological values which could be classified as healthy according to Bendixen's leukosis key.

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Section: Introductionmentioning

confidence: 71%

Sero-epidemiological studies of bovine leukaemia virus infection in Indian cross-bred zebu cattle

Singh¹,

Bansal²,

Singh³

1987

Rev. Sci. Tech. OIE

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“…It is spherical in shape with a diameter of 60-125 nm (Calafat et al . , 1974; Calafat and Ressang, 1977aRessang, , 1977bDutta et al . , 1970;Miller et al .…”

Section: Bovine Leukemia Virusmentioning

confidence: 99%

Epidemiology of natural transmission of bovine leukemia virus infection / by Mark Cy Thurmond.

Thurmond¹

1982

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A 27-month study examined 473 dairy cattle for associations between bovine leukemia virus (BLV) infection and host and environmental factors. Cattle sera were tested at monthly intervals for BLV antibodies by agar-gel immunodiffusion using the glycoprotein-51 antigen. A model of BLV colostral antibody decay in 130 calves predicted infection in calves less than six months of age and estimated antibody half-life to be 27.1 ±1.2 days. Colostral antibody decay did not differ between BLV-infected and noninfected calves for slope (p = 0.45) or intercept (p = 0.43). By 95 days of age, 50% of the calves had no detectable BLV colostral antibodies. Of 125 calves born to BLV-infected cows and followed for at least four months, eight (6.4%) had precolostral BLV antibodies, as determined by radioimmunoassay using the glycoprotein-51 antigen-In utero infection with BLV was not associated with dam age (p = 0.86), dam parity (p = 0.83), breed (p = 0.66), sex (p = 0.11), or stage of gestation in which the dam was infected (p=0.50). Calves infected in utero did not pose an increased risk of infection to calves penned next to them (p = 0.61). Prevalence rates of infection were 9%, 16%, and 63% at 6, 16, and 27 months of age, respectively. Age-specific rates of infection were not associated with dam age (p = 0.79), dam parity (p=0.75), dam BLV-status (p = 0.46), breed (p = 0.86), or BLV-status of colostrum consumed (p=0.50). An algorithm was described which allocated probabilities of infection to locations occupied by an animal prior to detection of infection. Small calf pastures were associated with less infection than was the calf barn (p< 0.05). No less infection was associated with individual outdoor calf pens compared to contiguous indoor pens (p>0.05). Risk of infection associated with the dry herd was five times that for heifer pastures (p< 0.0001) and accounted 24 infections per 100 heifers per 100 days at risk. Vaccination for infectious diseases was not associated with increased BLV infection (p=0.33). Infection rates were not associated with month of birth (p = 0.24) or with season of potential arthropod vectors (p = 0.20). Heifer infection was likely to occur in late v/inter or spring (p =. 01). Interacting Computing (MUSIC) version 4.1, 197 8.

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“…Electron microscopic studies have demonstrated C-type particles in the lymphocyte cultures of cattle with leukemia (3,4,13,23).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterisation of oncornavirus from primary cultures of tissues from cattle with leukemia

Parfanovich

Kasak

Баранов

et al. 1977

Archives of Virology

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The purpose of this investigation was to search for oncornavirus in primary cell cultures obtained from leukemic cattle organs and lymphocytes and to study their molecular-biological properties and role in the etiology of cattle leukemia. The investigation was carried out on 25 primary trypsinized cell culutres of lymph nodes, spleens, kidneys and lymphocytes from cattle with acute and chronic leukemia. It was demonstrated that all cell cultures from leukemic cattle (in contrast to cell cultures from healthy cattle) released oncornavirus into the culture medium. The virus possesses the main properties of oncornaviruses: it has a virion of C-type structure with a density of 1.16--1.18g/ml in a 20--60 per cent sucrose gradient, which may be induced by 5-bromodeoxyuridine, inhibited by Actino-mycin D, has reverse transcriptase activity, contains 60S RNA, that is annealed in the reaction of molecular hybridization with DNA of lymph nodes of cattle with leukemia. The propagation of the isolated oncornavirus in continuous cell lines of calf kidney culture was demonstrated. Experimental inoculation of purified oncornavirus was carried out on 60 baby calves and 15 lambs from leukosis free herds or flocks. Several of the calves later showed evidence of virus infection.

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Isolation of C Type Virus Particles From Leukemic and Lymphocytotic Cattle.

Cited by 5 publications

References 0 publications

Sero-epidemiological studies of bovine leukaemia virus infection in Indian cross-bred zebu cattle

Sero-epidemiological studies of bovine leukaemia virus infection in Indian cross-bred zebu cattle

Epidemiology of natural transmission of bovine leukemia virus infection / by Mark Cy Thurmond.

Isolation and characterisation of oncornavirus from primary cultures of tissues from cattle with leukemia

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