Isolation of Bloodstream Trypomastigotes of Trypanosoma cruzi by a Gradient of Metrizamide

Loures, M. A.; Pimenta, P. F. P.; Souza, Wanderley de

doi:10.2307/3280420

Cited by 14 publications

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“…T Trypanosoma cruzi has been limited to anion exchange chromatography and density gradient centrifugation (17). Early studies (4, 8,14,[26][27][28] using analytical microelectrophoresis indicated surface charge differences exist among various stages and species of trypanosomes and host cells.…”

Section: He Application Of Physical Cell Separation Techniques Tomentioning

confidence: 99%

Separation of Individual Stages of Trypanosoma cruzi Grown in Cell Culture by Continuous Free‐Flow Electrophoresis

Murray

Boltz

Schmatz

1982

The Journal of Protozoology

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The separation of extracellular protozoan parasites from host cells based on a difference in surface charge has been described. However, with Trypanosoma cruzi no method exists for the isolation of pure parasite stages from heterogeneous mixtures. Studies on the electrophoresis of mixed stage populations confirm significant surface charge density differences exist among epimastigotes, trypomastigotes, and amastigotes. In ascending order of electronegativity, amastigotes have the lowest charge density, trypomastigotes next, followed by epimastigotes. A technique has been developed for the separation of purified populations of parasites based on these charge differences using a continuous free-flow electrophoresis apparatus. The separated populations are morphologically intact and maintain their infectivity to mice. This separation method is applicable for preparative and analytical isolation of pure stages of T. cruzi for biochemical and immunological studies.

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Section: He Application Of Physical Cell Separation Techniques Tomentioning

confidence: 99%

Separation of Individual Stages of Trypanosoma cruzi Grown in Cell Culture by Continuous Free‐Flow Electrophoresis

Murray

Boltz

Schmatz

1982

The Journal of Protozoology

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“…Density-gradients of metrizamide, a tri-iodinated derivative of benzoic acid which dissolves readily in water and gives solutions of low viscosity and osmolarity, have been used in the isolation of several types of cells and subcellular particles (Rickwood & Birnie, 1975) including erythrocytes infected with murine plasmodia (Eugui & Allison, 1979) and trypomastigotes of Trypanosoma cruzi (Loures, Pimenta & de Souza, 1980). This paper describes a method for purifying sporozoites of Eimeria spp.…”

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confidence: 99%

The large-scale preparation of purified sporozoites of Eimeria spp. by metrizamide density-gradient centrifugation

Wisher¹,

Rose²

1984

Parasitology

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Purification of metacyclic trypomastigotes ofTrypanosoma cruzi andTrypanosoma dionisii from culture using an epimastigote-specific monoclonal antibody

1987

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Monoclonal antibodies which react with culture forms of Trypanosoma dionisii and Trypanosoma cruzi were tested for their agglutination capacity. In these studies developmental stage specificity for the epimastigote form could be observed. This specificity meant it was possible to develop a quick and simple method to isolate cultured metacyclic trypomastigotes of T. dionisii and T. cruzi group 2. After agglutination of the epimastigote form with the monoclonal antibody Dion 4.6 the purity of metacyclic trypomastigote developmental forms derived from culture was 96% to 99%.

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Isolation of Bloodstream Trypomastigotes of Trypanosoma cruzi by a Gradient of Metrizamide

Cited by 14 publications

References 0 publications

Separation of Individual Stages of Trypanosoma cruzi Grown in Cell Culture by Continuous Free‐Flow Electrophoresis

Separation of Individual Stages of Trypanosoma cruzi Grown in Cell Culture by Continuous Free‐Flow Electrophoresis

The large-scale preparation of purified sporozoites of Eimeria spp. by metrizamide density-gradient centrifugation

Purification of metacyclic trypomastigotes ofTrypanosoma cruzi andTrypanosoma dionisii from culture using an epimastigote-specific monoclonal antibody

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