Isolation of bacteriophages specific for the genus Veillonella

Hiroki, H; Shiiki, J.; Handa, Ayami; Totsuka, Masayoshi; Nakamura, Osamu

doi:10.1016/0003-9969(76)90132-1

Cited by 14 publications

(13 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Fnp⌽02 was isolated from a saliva sample from a healthy individual, confirming that oral bacteriophages can be isolated from samples from the oral cavitities of people with and without periodontal disease (6,8,12,13,33,37). This is because in a bacterium-phage system, the phage could be found wherever its target bacterium is found, and oral pathogens can be found in both healthy and periodontally diseased individuals (26).…”

Section: Discussionmentioning

confidence: 56%

“…Although several bacteriophages have been isolated for a number of oral bacteria (8,10,12,13,23,33), there have been no reports on the isolation of a phage for fusobacteria until now. F. nucleatum, together with a bacterial consortium, generates the well-known dental plaque, a biofilm conformation that eventually leads to the onset of periodontal disease.…”

Section: Discussionmentioning

confidence: 99%

“…There are many examples of the use of bacteriophages at a clinical (14,32) and commercial level (20). Specifically in the dentistry area, several bacteriophages that infect diverse oral bacteria have been isolated from saliva and dental plaque (12,13,23,37). Although F. nucleatum is an important periodontal pathogen, reports of bacteriophages for this microorganism do not exist.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Isolation of a Novel Bacteriophage Specific for the Periodontal Pathogen Fusobacterium nucleatum

Machuca

Daille

Vinés

et al. 2010

Appl Environ Microbiol

View full text Add to dashboard Cite

Fusobacterium nucleatum is a periodontal pathogen that has been directly associated with the development and progression of periodontal disease, a widespread pathology that affects the support tissues of the tooth. We isolated a new bacteriophage (Fnp⌽02) that specifically infects this bacterium. Transmission electron microscopy showed that the virion is composed of an icosahedral head and a segmented tail. The size of the phage genome was estimated to be approximately 59 kbp of double-stranded DNA. The morphological features and the genetic characteristics suggest that Fnp⌽02 is part of the Siphoviridae family. Using one-step growth and adsorption experiments, the latent period, burst size, and adsorption rate were estimated to be 15 h, 100 infectious units per cell, and 7.5 ؋ 10 ؊10 ml min ؊1 , respectively. A small fragment of phage DNA was cloned and sequenced, showing 93% nucleotide identity with the phage PA6 of Propionibacterium acnes and amino acid identity with fragments of two proteins (Gp3 and Gp4) of this phage. To our knowledge, Fnp⌽02 is the first phage described to infect Fusobacterium nucleatum and provides the base for future exploration of phages in the control of periodontal disease.

show abstract

Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Isolation of a Novel Bacteriophage Specific for the Periodontal Pathogen Fusobacterium nucleatum

Machuca

Daille

Vinés

et al. 2010

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Fresh dental plaques may contain phages infecting Actinomyces [54]. Furthermore, phages against Lactobacillus, Veilonella, Actinobacillus and Streptococcus mutans have been obtained from oral material [55,56]. Bachrach et al [57] have found Enterococcus faecalis phages in human saliva and suggested that they can have a role in the oral ecosystem by restricting this bacterial population to the tooth root system (Enterobacter faecalis can frequently be isolated from root canals where endodonthic treatment has failed [58]).…”

Section: Phage Presence At Sites Outside the Gastrointestinal Tractmentioning

confidence: 99%

The potential role of endogenous bacteriophages in controlling invading pathogens

Górski

Weber‐Dąbrowska

2005

CMLS, Cell. Mol. Life Sci.

137

View full text Add to dashboard Cite

Bacteriophages (phages) are omnipresent in our environment, and recent studies highlight their potential impact on the microbial world. Phages can also be present in mammalian organisms, including man (intestines, oral cavity, urine, sputum and serum). Data are available which suggest that those endogenous phages could play an important role in eliminating bacteria and regulating the body ecosystem. Furthermore, our most recent findings suggest that phages can exert immunosuppressive action in the gut, helping control local inflammatory and autoimmune reactions, and demonstrate anticancer activity. We hypothesize that phages could act in concert with the immune system in immunosurveillance against bacteria, viruses and cancer.

show abstract

“…1958) and a range of bacteriophages specific for species of Veillonella spp. were isolated by Hiroki et al. (1976).…”

Section: Introductionmentioning

confidence: 99%

Isolation of bacteriophages from the oral cavity

Hitch

Pratten

Taylor

2004

Lett Appl Microbiol

View full text Add to dashboard Cite

Aims: To isolate bacteriophages lytic for oral pathogens from human saliva, dental plaque and mature biofilms constituted from saliva-derived bacteria. Methods and Results: Saliva and dental plaque samples from healthy volunteers and from patients with gingivitis and periodontitis were examined for the presence of lytic bacteriophage using a panel of oral pathogens and bacteria isolated from the samples. Samples were also enriched for bacteriophage using static culture techniques and mature biofilms. A limited number of samples contained bacteriophage particles that were visualized using electron microscopy. Cultures yielded phage infecting non-oral bacteria (Proteus mirabilis) but no bacteriophage specific for recognized oral pathogens were found. Some micro-organisms from the oral microflora elaborated antibacterial substances that inhibited growth of other residents of the oral cavity. Conclusions: Unlike other ecosystems, the composition of the oral cavity does not appear to be heavily influenced by interactions between bacteriophages and their hosts. Significance and Impact of the Study: Bacteriophage for control of oral infections may need to be obtained from other sources. Antibacterial substances derived from some members of the oral microflora warrant investigation as potential antibiotics.

show abstract

Isolation of bacteriophages specific for the genus Veillonella

Cited by 14 publications

References 3 publications

Isolation of a Novel Bacteriophage Specific for the Periodontal Pathogen Fusobacterium nucleatum

Isolation of a Novel Bacteriophage Specific for the Periodontal Pathogen Fusobacterium nucleatum

The potential role of endogenous bacteriophages in controlling invading pathogens

Isolation of bacteriophages from the oral cavity

Contact Info

Product

Resources

About