Isolation of bacterial endophytes from Actinidia chinensis and preliminary studies on their possible use as antagonists against Pseudomonas syringae pv. actinidiae

Tontou, Rodanthi; Giovanardi, Davide; Ferrari, Michele; Stefani, Emilio

doi:10.3233/jbr-160118

Cited by 18 publications

(10 citation statements)

References 32 publications

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“…A possible and safe alternative to manage Monilinia fruit decays is biological control using antagonistic microorganisms often isolated from food sources and from natural environment in which they compete with pathogenic epiphytic microorganisms (Grzegorczyk et al, 2017;Tontou et al, 2016a). Also bacterial endophytes, which provide important benefits to plants, might be deserved for developing innovative and sustainable biocontrol strategies (Compant et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…P. synxantha DLS65, isolated from kiwi tissues in the region of Emilia Romagna, Italy (Tontou et al, 2016a), and genotipically characterized (Tontou et al, 2016b), was maintained in 15% glycerol at -80°C and subcultured on Nutrient Agar medium (NA, Oxoid, Basingstoke, UK) at 25 ± 1°C.…”

Section: Pseudomonasstrainmentioning

confidence: 99%

“…In addition, P. synxantha strain DLS65, isolated from vascular tissues of Actinidia spp. plants, showed strong in vitro inhibition activity against Gram + and Gram -phytopatogenic bacteria (Tontou et al, 2016a). Therefore the aims of the present study were i) to determine antagonistic activity of DLS65 strain against Monilia fructicola and Monilia fructigena in vitro and ii) to evaluate the potential biocontrol activity of brown rot caused by these pathogens in postharvest on peach fruit by comparison with a standard fungicide.…”

Section: Introductionmentioning

confidence: 99%

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Postharvest biocontrol ability of Pseudomonas synxantha against Monilinia fructicola and Monilinia fructigena on stone fruit

Aiello

Restuccia

Stefani

et al. 2019

Postharvest Biology and Technology

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The biocontrol properties of the endophyte Pseudomonas synxantha DLS65 were tested in vitro and in vivo against Monilinia fructicola and Monilinia fructigena, causal agents of postharvest brown rot of stone fruit. P. synxantha cells significantly reduced the mycelial growth of both pathogens on Potato Dextrose Agar (PDA), and strongly inhibited the Monilinia fructicola growth on Peach Extract Agar (PEA). Cell-free culture filtrates inhibited the pathogens on PDA and PEA to lesser extent. The production of volatile organic compounds (VOCs), with in vitro inhibitory effects on mycelial growth, was also observed. P. synxantha significantly reduced brown rot incidence and severity on peach fruit artificially inoculated with M. fructicola after 5 d at 25°C. Moreover, P. synxantha more significantly reduced incidence and severity after 10 d at 10°C and after 20 d in cold storage at 0°C in comparison to control fruit, even if its activity was never comparable to that of the synthetic fungicide Scholar ® (fludioxonil). Similarly, P. synxantha exhibited an excellent antagonistic activity against M. fructigena on fruit at 10 and at 0°C, and a weak biocontrol activity at 25°C. Competition for nutrients and space, production of diffusible toxic metabolites and VOCs may play a role in the antagonism of P. synxantha toward M. fructicola and M. fructigena, especially at the lowest temperatures of storage. For that reason, this strain of P. synxantha could be suggested as active ingredient for the setting up of bioformulates against Monilinia species representing a limiting factor for stone fruit production.

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Section: Discussionmentioning

confidence: 99%

Section: Pseudomonasstrainmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Postharvest biocontrol ability of Pseudomonas synxantha against Monilinia fructicola and Monilinia fructigena on stone fruit

Aiello

Restuccia

Stefani

et al. 2019

Postharvest Biology and Technology

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“…Biocontrol strategies, such as the use of antagonistic bacteria, like Bacillus subtilis, Pantoea agglomerans , and Lactobacillus plantarum , have shown promising results against Psa in in vitro conditions [ 14 , 15 , 16 ]; however, the efficacy of this approach to control Psa infection in commercial field conditions was less consistent [ 15 , 17 , 18 , 19 ]. Bacteriophages have also been assayed as controlling agents against phytopathogens.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Bacteriophages against Pseudomonas Syringae pv. Actinidiae with Potential Use as Natural Antimicrobials in Kiwifruit Plants

et al. 2020

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Pseudomonas syringae pv. actinidiae (Psa) is the causal agent of a bacterial canker in kiwifruit plants and has caused economic losses worldwide. Currently, the primary strategies to control this pathogen include the use of copper-based compounds and even antibiotics. However, the emergence of isolates of Psa that are resistant to these agrochemicals has raised the need for new alternatives to control this pathogen. Bacteriophages have been proposed as an alternative to control bacterial infections in agriculture, including Psa. Here, we show the isolation and characterization of 13 phages with the potential to control Psa infections in kiwifruit plants. The phages were characterized according to their host range and restriction fragment length polymorphism (RFLP) pattern. Four phages were selected according to their lytic effect on the bacteria and their tolerance to different environmental conditions of pH (4–7), temperature (4–37 °C), and solar radiation exposure (30 and 60 min). The selected phages (CHF1, CHF7, CHF19, and CHF21) were sequenced, revealing a high identity with the podophage of Psa phiPSA2. In vitro assays with kiwifruit leaf samples demonstrated that the mixture of phages reduced the Psa bacterial load within three hours post-application and was able to reduce the damage index in 50% of cases. Similarly, assays with kiwifruit plants maintained in greenhouse conditions showed that these phages were able to reduce the Psa bacterial load in more than 50% of cases and produced a significant decrease in the damage index of treated plants after 30 days. Finally, none of the selected phages were able to infect the other bacteria present in the natural microbiota of kiwifruit plants. These results show that bacteriophages are an attractive alternative to control Psa infections in kiwifruit plants.

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“…Present studies of Psa are mainly focused on species classification [ 7 , 44 ], molecular biology [ 45 – 47 ], analysis of biological characteristics [ 48 , 49 ], pathogenicity differentiation[ 50 – 52 ], rapid detection methods [ 48 , 53 , 54 ], and disease control [ 55 , 56 ]; however, systemic research about the influence of climate change on the niches specific to Psa is lacking. In an effort to analyze the effects of climate change on the potential distribution of Psa, MaxEnt was utilized to model the current niches of Psa in China, as well as the future Psa niches under climate change scenarios; additionally, this study identified climatic variables important for the potential establishment of Psa.…”

Section: Introductionmentioning

confidence: 99%

Modeling and mapping the current and future distribution of Pseudomonas syringae pv. actinidiae under climate change in China

Wang

He³

et al. 2018

PLoS ONE

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ObjectiveBacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae (Psa) is a major threat to the kiwifruit industry throughout the world and accounts for substantial economic losses in China. The aim of the present study was to test and explore the possibility of using MaxEnt (maximum entropy models) to predict and analyze the future large-scale distribution of Psa in China.MethodBased on the current environmental factors, three future climate scenarios, which were suggested by the fifth IPCC report, and the current distribution sites of Psa, MaxEnt combined with ArcGIS was applied to predict the potential suitable areas and the changing trend of Psa in China. The jackknife test and correlation analysis were used to choose dominant climatic factors. The receiver operating characteristic curve (ROC) drawn by MaxEnt was used to evaluate the accuracy of the simulation.ResultThe results showed that under current climatic conditions, the area from latitude 25° to 36°N and from longitude 101° to 122°E is the primary potential suitable area of Psa in China. The highly suitable area (with suitability between 66 and 100) was mainly concentrated in Northeast Sichuan, South Shaanxi, most of Chongqing, West Hubei and Southwest Gansu and occupied 4.94% of land in China. Under different future emission scenarios, both the areas and the centers of the suitable areas all showed differences compared with the current situation. Four climatic variables, i.e., maximum April temperature (19%), mean temperature of the coldest quarter (14%), precipitation in May (11.5%) and minimum temperature in October (10.8%), had the largest impact on the distribution of Psa.ConclusionThe MaxEnt model is potentially useful for forecasting the future adaptive distribution of Psa under climate change, and it provides important guidance for comprehensive management.

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Isolation of bacterial endophytes from Actinidia chinensis and preliminary studies on their possible use as antagonists against Pseudomonas syringae pv. actinidiae

Cited by 18 publications

References 32 publications

Postharvest biocontrol ability of Pseudomonas synxantha against Monilinia fructicola and Monilinia fructigena on stone fruit

Postharvest biocontrol ability of Pseudomonas synxantha against Monilinia fructicola and Monilinia fructigena on stone fruit

Characterization of Bacteriophages against Pseudomonas Syringae pv. Actinidiae with Potential Use as Natural Antimicrobials in Kiwifruit Plants

Modeling and mapping the current and future distribution of Pseudomonas syringae pv. actinidiae under climate change in China

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