Isolation of Bacillus sp. strains capable of decomposing alkali lignin and their application in combination with lactic acid bacteria for enhancing cellulase performance

Abstract

Effective biological pretreatment method for enhancing cellulase performance was investigated. Two alkali lignin-degrading bacteria were isolated from forest soils in Japan and named CS-1 and CS-2. 16S rDNA sequence analysis indicated that CS-1 and CS-2 were Bacillus sp. Strains CS-1 and CS-2 displayed alkali lignin degradation capability. With initial concentrations of 0.05-2.0 g L(-1), at least 61% alkali lignin could be degraded within 48 h. High laccase activities were observed in crude enzyme extracts from the isolated strains. This result indicated that alkali lignin degradation was correlated with laccase activities. Judging from the net yields of sugars after enzymatic hydrolysis, the most effective pretreatment method for enhancing cellulase performance was a two-step processing procedure (pretreatment using Bacillus sp. CS-1 followed by lactic acid bacteria) at 68.6%. These results suggest that the two-step pretreatment procedure is effective at accelerating cellulase performance.

Thermal Dynamics and a Comparison of the Thermal Stability of Various Non-Wood Pulps

“…These results were in agreement with previous research which showed that pure cellulose has better thermal stability than lignocellulosic fibers (Chang et al 2014). Therefore, the chemical compositions of pulp were the main criteria in deciding the thermal stability of pulp.…”

Extraction of ligninolytic enzymes from novel Klebsiella pneumoniae strains and its application in wastewater treatment

“…Similarly, Bacillus sp. strains CS-1&CS-2 removed 61% of lignin (Chang et al 2014). In another finding that corroborated to this work, 50% and 55% of lignin were degraded by Citrobacter freundii and Serratia marcescens, respectively.…”

“…strain CS-1 (3.5 U/L) and CS-2 (3.0 U/L). These strains showed very low MnP activity (data not shown), while LiP activity was not observed (Chang et al 2014). In contrast to the results obtained in present study, Lai and co-worker worked on thermophilic lignin-degrading bacteria and reported that out of ten isolates CLMT 18 (0.7475 U/ml) and CLMT 34 (0.8178 U/ml) showed the maximum laccase activity, while CLMT5 (1.8759 U/ml), CLMT 18 (1.6684 U/ ml) and CLMT 20 (1.5322 U/ml) showed maximum MnP activity (Lai et al 2017).…”

Photocatalytic degradation of lignin on synthesized Ag–AgCl/ZnO nanorods under solar light and preliminary trials for methane fermentation

“…Most recently, some efforts have been done to find effective methods to degrade lignin for value-added biofuels and chemicals production, including enzymatic degradation (Chen et al, 2012) alkali pyrolysis (Guo et al, 2014), hydrothermal conversion (Barbier et al, 2012), electrochemical degradation (Tolba et al, 2010), biological degradation (Chang et al, 2014), photocatalytic degradation (Prado et al, 2013), etc. Among them, photocatalysis is considered as an environment friendly and promising technology due to excellent merits such as clean, effective, energy-saving, and low cost.…”