Isolation of Bacillus HB–1 from Human Clinical Sources

Bottone, Edward J.; Kittick, John; Schneierson, S. Stanley

doi:10.1093/ajcp/59.4.560

Cited by 41 publications

(13 citation statements)

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“…Eikenella corrodens, a fastidious, slow-growing, facultatively anaerobic gram-negative rod that is part of the normal oral and fecal flora of humans (2) has been increasingly recognized as a pathogen in human infections (1)(2)(3)(4)(5)(6)(7)(8)(9)11 The activity of the 13 antimicrobial agents tested against the 28 strains of E. corrodens is shown in Table 1. All strains of E. corrodens tested were uniformly susceptible to cefoxitin (-1 ,ug/ml), cefotaxime (sl ,ug/ml), moxalactam ('1 ,ug/ml), N-fomiimidoyl thienamycin (c 0.25 ,ug/ml), ticarcillin (c4 ,ug/ml), and penicillin G ('4 ,ug/ml) and resistant to clindamycin.…”

mentioning

confidence: 99%

Susceptibility of Eikenella corrodens to newer beta-lactam antibiotics

Goldstein¹,

Gombert²,

Agyare³

1980

Antimicrob Agents Chemother

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In vitro susceptibility testing of 28 strains of Eikenella corrodens by the agar dilution technique showed that all strains were uniformly susceptible to penicillin, ticarcillin, cefoxitin, cefotaxime, N-formimidoyl thienamycin, and moxalactam and resistant to clindamycin and cefadroxil. Cefoperazone, piperacillin, and mezlocillin showed good activity, with some strains relatively resistant. Bacampicillin and cefamandole showed relatively poor activity.Eikenella corrodens, a fastidious, slow-growing, facultatively anaerobic gram-negative rod that is part of the normal oral and fecal flora of humans (2) has been increasingly recognized as a pathogen in human infections (1-9, 11). It is variably susceptible to beta-lactam and aminoglycoside antibiotics; it is uniformly susceptible to penicillin and ampicillin and resistant to penicillinase-resistant penicillins, clindamycin, and metronidazole (1, 2, 10, 11). We determined the in vitro susceptibility, by the agar dilution technique, of 28 strains of E. corrodens, all clinical isolates, to the newer beta-lactam antibiotics and to penicillin and clindamycin.All isolates were identified by standard criteria (1, 2). The sources and numbers of the isolates were as follows: abscesses, eight; transtrachial aspirates, five; human bite wounds, five; other wounds, five; bile, one; blood, one; urine, one; and sputum, two. Laboratory powders were kindly supplied by the following: cefoxitin and N-formimidoyl thienamycin, Merck & Co., Inc., Rahway, N. The activity of the 13 antimicrobial agents tested against the 28 strains of E. corrodens is shown in Table 1. All strains of E. corrodens tested were uniformly susceptible to cefoxitin (-1 ,ug/ml), cefotaxime (sl ,ug/ml), moxalactam ('1 ,ug/ml), N-fomiimidoyl thienamycin (c 0.25 ,ug/ml), ticarcillin (c4 ,ug/ml), and penicillin G ('4 ,ug/ml) and resistant to clindamycin. Susceptibility to the other agents was variable. Cefoperazone, piperacillin, and mezlocillin showed good activity, with some strains relatively resistant. Cefadroxil showed poor activity (32 to 128 ,ug/ml); the activity of cefamandole was relatively poor (4 to 16 ,ug/ml), as was that of bacampicillin (2 to 8 ,ug/ml).The extended-spectrum beta-lactam antibiotics are frequently used as initial empiric therapy for a wide variety of clinical situations in which E. corrodens is a potential pathogen. Consequently, the susceptibility pattem of E. corrodens must be considered when a therapeutic reginen is chosen.Our data are in agreement with prior reports that E. corrodens is susceptible to penicillin and cefoxitin but resistant to clindamycin (1, 2, 10, 11). On a weight basis, cefoxitin, cefotaxime, moxalactam, and N-formimidoyl thienamycin showed better activity than did penicillin against all strains tested. Ticarcillin showed comparable activity. Cefoperazone, piperacillin, and mezlocillin showed good activity, but some strains were relatively resistant.

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mentioning

confidence: 99%

Susceptibility of Eikenella corrodens to newer beta-lactam antibiotics

Goldstein¹,

Gombert²,

Agyare³

1980

Antimicrob Agents Chemother

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“…Despite microbiologists' increasing awareness of the pathogenic potential of E. corrodens (1,2,12), it is easily missed on routine culture. This slow-growing gram-negative rod produces pinpoint colonies even after 24 to 48 h of incubation, is usually isolated in mixed culture, and is therefore frequently overgrown by other organisms (2, 9,12).…”

Section: Discussionmentioning

confidence: 99%

“…E. corrodens grows better in a 5% CO2 atmosphere, requires hemin for growth in aerobic and 5% CO2 but not anaerobic atmospheres of incubation, and fails to grow on eosin methylene blue or MacConkey agar or other media selective for gram-negative rods (9). Information regarding the growth of E. corrodens on currently available commercial and laboratory media under various atmospheric conditions remains sparse (1,4,9). Reports of the clinical isolation or antimicrobial susceptibility testing of E. corrodens are not uniform because of the use of diverse media and atmospheres of incubation (1-3, 8, 10-12).…”

Section: Discussionmentioning

confidence: 99%

Comparative growth of Eikenella corrodens on 15 media in three atmospheres of incubation

Goldstein¹,

Agyare²,

Silletti³

1981

J Clin Microbiol

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The abundance of growth and size of colonies of 10 isolates of Eikenella corrodens were studied using 15 commonly available media in aerobic, 5% CO2, and anaerobic atmospheres of incubation. Chocolate agar was the best medium for overall growth in all three atmospheres. Growth on Mueller-Hinton agar supplemented with 5% blood and on Brucella agar supplemented with 5% blood was equivalent to that on chocolate agar in 5% CO2 and anaerobic environments but not in an aerobic atmosphere. Strains of E. corrodens did not grow on the selective media studied. Growth on media without blood or hemin supplements occurred in anaerobic but not in aerobic or 5% CO2 environments, and was consistently poorer than growth on the same media with these supplements.

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“…Additional infections noted in the latter series included abdominal abscess, otitis media, and a reaction from a human bite. The organism was isolated in pure culture in 17 cases of infected frontal sinuses, wounds, and peritoneal fluid, and in mixed flora from abscesses and sputum (Bottone et al, 1973).…”

Section: Discussionmentioning

confidence: 99%