Isolation of avian pneumovirus from mallard ducks that is genetically similar to viruses isolated from neighboring commercial turkeys

Shin, Hyun Jin; Nagaraja, K. V.; McComb, Brian; Halvorson, David A.; Jirjis, Faris F.; Shaw, Daniel P.; Seal, Bruce S.; Njenga, M. Kariuki

doi:10.1016/s0168-1702(01)00402-6

Cited by 33 publications

(28 citation statements)

References 11 publications

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“…1). The high incidence of APV in spring (April to May) and autumn (October to December) may have been due to the migratory patterns of wild birds, which according to recent studies may be involved in spreading the virus (38,39). RNA was isolated from nasal turbinate samples of wild sparrows, geese, swallows, and starlings (39).…”

Section: Resultsmentioning

confidence: 99%

Molecular Epidemiology of Subgroup C Avian Pneumoviruses Isolated in the United States and Comparison with Subgroup A and B Viruses

et al. 2002

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The avian pneumovirus (APV) outbreak in the United States is concentrated in the north-central region, particularly in Minnesota, where more outbreaks in commercial turkeys occur in the spring (April to May) and autumn (October to December). Comparison of the nucleotide and amino acid sequences of nucleoprotein (N), phosphoprotein (P), matrix (M), fusion (F), and second matrix (M2) genes of 15 U.S. APV strains isolated between 1996 and 1999 revealed between 89 and 94% nucleotide sequence identity and 81 to 95% amino acid sequence identity. In contrast, genes from U.S. viruses had 41 to 77% nucleotide sequence identity and 52 to 78% predicted amino acid sequence identity with European subgroup A or B viruses, confirming that U.S. viruses belonged to a separate subgroup. Of the five proteins analyzed in U.S. viruses, P was the most variable (81% amino acid sequence identity) and N was the most conserved (95% amino acid sequence identity). Phylogenetic comparison of subgroups A, B, and C viruses indicated that A and B viruses were more closely related to each other than either A or B viruses were to C viruses.The Turkey rhinotracheitis virus, commonly referred to as avian pneumovirus (APV), is a member of the Paramyxoviridae family that causes acute rhinotracheitis in turkeys that is characterized by coughing, nasal discharge, tracheal rales, foamy conjunctivitis, and sinusitis in turkeys of all ages. In laying birds, there is a transient drop in egg production, along with mild respiratory tract illness (11). Uncomplicated cases have low mortality (2 to 5%), but APV infections accompanied by secondary infections (bacterial and/or viral) can result in up to 25% mortality (11). APV was first detected in South Africa in 1978 but was isolated soon thereafter in the United Kingdom,

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Section: Resultsmentioning

confidence: 99%

Molecular Epidemiology of Subgroup C Avian Pneumoviruses Isolated in the United States and Comparison with Subgroup A and B Viruses

et al. 2002

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“…Oceania and Canada are the only regions that have not reported aMPV (Cook, 2000;Gough, 2003). There is serological and molecular evidence that aMPV occurs in a variety of other avian species, including pheasants, guinea fowl, ostriches, passerines and various waterfowl (Shin et al, 2002;Gough, 2003;Bennett et al, 2004;Lee et al, 2007), but there is no evidence of disease (Pedersen and Gough, 2009).…”

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confidence: 99%

Seroprevalence of avian metapneumovirus infection in broiler and broiler breeder chickens in Iran

Rahimi¹

2011

Vet. Med. - Czech

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Avian metapneumovirus causes an acute highly contagious upper respiratory tract infection primarily of turkeys and chickens. The disease can cause significant economic losses in turkey and chicken flocks, particularly when exacerbated by secondary pathogens. The purpose of this study was to determine the prevalence of avian metapneumovirus antibodies in broiler and broiler breeder flocks in Kermanshah province, west of Iran. All the flocks had not been vaccinated against avian metapneumovirus. The province were divided into four geographic areas; southwest, southeast, northwest, and northeast. Flocks in each area, and 14-15 birds in each flock, were randomly sampled. The blood samples were taken regardless of the presence of any signs of respiratory or any other clinical disease in the flocks. A total of 435 blood samples were collected from 30 commercial chicken flocks (24 broiler flocks, aged between six and eight weeks, and six broiler breeder flocks, aged between 56 and 72 weeks). The presence of antibodies against avian metapneumovirus in each serum sample was tested twice by enzyme-linked immunosorbent assay using a commercial kit which was able to determine antibodies against A, B and C subtypes of avian metapneumovirus. Out of 347 serum samples obtained from broiler chickens, 167 (48.1%) were positive to avian metapneumovirus antibodies, which represented 20 (83.3%) of 24 examined broiler flocks. Out of 88 samples obtained from broiler breeder chickens, 82 (93.2%) were positive to avian metapneumovirus antibodies, which belonged to six (100%) of examined broiler breeder flocks. Detection of anti-avian metapneumovirus antibodies among broiler breeder (100%) was higher than broiler (83.3%) flocks. A higher rate of seropositivity (83.3% of samples and 100% of broiler flocks) was observed in northwest. The results of this study may indicate the possible involvement of avian metapneumovirus in the respiratory disease we are seeing in chickens in Iran. Its prevalence has to be investigated in other parts of Iran. Future work may and should include the use of molecular methods and isolation of the virus. Isolation of avian metapneumovirus will allow the possibility of making autogenous vaccines.

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“…The aMPV strains isolated from the United States were a distinct subtype C, genetically different from the subtypes A (aMPV/A), B (aMPV/B), and D (aMPV/D) circulating in Europe, Asia, Africa, and South America (2,22,23,24,34,38,46,47,48). Approximately 80% of the aMPV outbreaks in the United States occur in spring (March to May) and autumn (October to November), corresponding to periods of wild bird migration, resulting in the hypothesis that wild birds may be involved with the transmission of virus between commercial turkey farms (4,11,38,39). In support of this hypothesis, aMPV RNA was isolated from the nasal turbinates of wild sparrows, geese, blue-winged teal, and starlings and shown to share 90 to 95% nucleotide sequence identity with viruses isolated from domestic turkeys (3,4,37).…”

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confidence: 99%

“…In support of this hypothesis, aMPV RNA was isolated from the nasal turbinates of wild sparrows, geese, blue-winged teal, and starlings and shown to share 90 to 95% nucleotide sequence identity with viruses isolated from domestic turkeys (3,4,37). In addition, an infectious aMPV was isolated from sentinel ducks housed in close proximity to an aMPV-infected turkey farm, and the virus also had high nucleotide sequence identity with turkey viruses (39). We recently isolated four strains of aMPV (goose 15a/01, goose 15b/01, goose 15c/01, and goose 15d/01) from asymptomatic wild Canada geese captured in Minnesota (3).…”

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A Wild Goose Metapneumovirus Containing a Large Attachment Glycoprotein Is Avirulent but Immunoprotective in Domestic Turkeys

Bennett

LaRue

Shaw

et al. 2005

J Virol

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The genomic structure and composition of an avian metapneumovirus (aMPV) recently isolated from wild Canada geese (goose 15a/01) in the United States, together with its replication, virulence, and immunogenicity in domestic turkeys, were investigated. The sizes of seven of the eight genes, sequence identity, and genome organization of goose aMPV were similar to those of turkey aMPV subtype C (aMPV/C) strains, indicating that it belonged to the subtype. However, the goose virus contained the largest attachment (G) gene of any pneumovirus or metapneumovirus, with the predicted G protein of 585 amino acids (aa) more than twice the sizes of G proteins from other subtype C viruses and human metapneumovirus and more than 170 aa larger than the G proteins from the other aMPV subtypes (subtypes A, B, and D). The large G gene resulted from a 1,015-nucleotide insertion at 18 nucleotides upstream of the termination signal of the turkey aMPV/C G gene. Three other aMPV isolates from Canada geese had similarly large G genes, whereas analysis of recent aMPV strains circulating in U.S. turkeys did not indicate the presence of the goose virus-like strain. In vitro, the goose virus replicated to levels (2 ؋ 10 5 to 5 ؋ 10 5 50% tissue culture infective dose) comparable to those produced by turkey aMPV/C strains. More importantly, the virus replicated efficiently in the upper respiratory tract of domestic turkeys but with no clinical signs in either day-old or 2-week-old turkeys. The virus was also horizontally transmitted to naïve birds, and turkey infections with goose 15a/01 induced production of aMPVspecific antibodies. Challenging day-old or 2-week-old turkeys vaccinated with live goose aMPV resulted in lower clinical scores in 33% of the birds, whereas the rest of the birds had no detectable clinical signs of the upper respiratory disease, suggesting that the mutant virus may be a safe and effective vaccine against aMPV infection outbreaks in commercial turkeys.

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Isolation of avian pneumovirus from mallard ducks that is genetically similar to viruses isolated from neighboring commercial turkeys

Cited by 33 publications

References 11 publications

Molecular Epidemiology of Subgroup C Avian Pneumoviruses Isolated in the United States and Comparison with Subgroup A and B Viruses

Molecular Epidemiology of Subgroup C Avian Pneumoviruses Isolated in the United States and Comparison with Subgroup A and B Viruses

Seroprevalence of avian metapneumovirus infection in broiler and broiler breeder chickens in Iran

A Wild Goose Metapneumovirus Containing a Large Attachment Glycoprotein Is Avirulent but Immunoprotective in Domestic Turkeys

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