Isolation of auxotrophic mutants and development of conjugation system in KISR methylotrophs

Abstract

Isolation of auxotrophic mutants and conjugal transfer of plasmids are successfully demonstrated in four obligate methylotrophic strains isolated a t Kuwait Institute for Scientific Research (KISR). Out of 11 auxotrophic, mutants, three required methionine and two leucine. Complete transfer of plasmid R68.45 from Pseudomoms to KISR methylotrophs and vice versa have been achieved with a low transfer rate.