Isolation of antigen/antibody complexes through organic solvent (ICOS) method

Akahori, Yasushi; Kurosawa, Gene; Sumitomo, Mariko; Muramatsu, Chiho; Eguchi, Kaoru; Tanaka, Miho; Suzuki, Kazuhiro; Sugiura, M.; Iba, Yoshitaka; Sugioka, Atsushi; Kurosawa, Yoshikazu

doi:10.1016/j.bbrc.2008.11.129

Cited by 21 publications

(23 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In BRASIL both selection and sorting of cell-surface-binding phage are based on differential centrifugation of cells with phage through an interface between aqueous and nonmiscible organic phases. The successful isolation of antibodies using this approach had been reported, for examples, CX 6 C phage peptides specific for vascular endothelial growth factor (VEGF) using human umbilical vein endothelial cells (HUVECs) [18] and phage antibodies against tumor associated antigens using human cancer cells [25]. The colony forming unit (cfu) of eluted phage determined after the cell panning indicated that specific phage enrichment had occurred in the primary antibody library utilizing HuL1, HuL2, HuL6 and HuL8 light chains (data not shown).…”

Section: Resultsmentioning

confidence: 98%

Isolation of human Fab antibodies specific for the low-affinity IgE receptor (CD23) by selecting a hierarchical antibody library system against B lymphoblastic IM-9 cells

Choi¹,

Song²,

Yoon³

et al. 2011

Immunology Letters

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 98%

Isolation of human Fab antibodies specific for the low-affinity IgE receptor (CD23) by selecting a hierarchical antibody library system against B lymphoblastic IM-9 cells

Choi¹,

Song²,

Yoon³

et al. 2011

Immunology Letters

View full text Add to dashboard Cite

“…We also thought so in the beginning, but gradually started to understand well the characteristics of the phage Ab system. Finally we succeeded in developing the ICOS (isolation of Ag/Ab complexes through organic solvent) method (8). In the case of the ICOS method while the first step for mixing of the cells and phage Abs in an aqueous solution is the same as that in the conventional method, the mixture is put on the organic layer and centrifuged.…”

Section: Identification Of Taas and Simultaneous Isolation Of Human Mmentioning

confidence: 99%

Comprehensive Identification of Tumor-associated Antigens via Isolation of Human Monoclonal Antibodies that may be Therapeutic

Kurosawa

2009

Immune Netw

Self Cite

View full text Add to dashboard Cite

Although the success of trastuzumab and rituximab for treatment of breast cancer and non-Hodgkins lymphoma, respectively, suggests that monoclonal antibodies (mAbs) will become important therapeutic agents against a wider range of cancers, useful therapeutic Abs are not yet available for the majority of the human cancers because of our lack of knowledge of which antigens (Ags) are likely to become useful targets. We established a procedure for comprehensive identification of such Ags through the extensive isolation of human mAbs that may be therapeutic. Using the phage-display Ab library we isolated a large number of human mAbs that bind to the surface of tumor cells. They were individually screened by immunostaining, and clones that preferentially and strongly stained the malignant cells were chosen. The Ags recognized by those clones were isolated by immunoprecipitation and identified by mass spectrometry (MS). We isolated 2,114 mAbs with unique sequences and identified 25 distinct Ags highly expressed on several carcinomas. Of those 2,114 mAbs 434 bound to specifically to one of the 25 Ags. I am going to discuss how we could select proper target Ags for therapeutic Abs and candidate clones as therapeutic agents.

show abstract

“…( 3 ) Identification of tumor‐associated Ag (TAA) and simultaneous isolation of human mAb against the Ag were established using the phage‐display Ab library, and these methodologies allowed us to obtain the Abs against Ag that are present on the surface of cancer cells and should be mediators of the carcinogenesis process. ( 4–6 ) In addition to the 21 identified TAA and 356 isolated mAb reported to date in a previous paper, ( 4 ) six more TAA and 140 human mAb were further identified and isolated, respectively. There is a need to select proper targets among the TAA and find potential candidate mAb for the development of therapeutic Ab against cancers.…”

mentioning

confidence: 91%

“…( 15 ) For screening of mAb, the ICOS method described by Akahori et al. (2008) ( 5 ) was followed. In addition to the 33 cell lines reported by Kurosawa et al.…”

mentioning

confidence: 99%

Selection and analysis of anti‐cancer antibodies for cancer therapy obtained from antibody phage library

et al. 2010

Self Cite

View full text Add to dashboard Cite

The search for effective antibodies (Ab) for curable cancer immunotherapy has been a quest of many research groups in order to find an effective target that exists on the cancer cell surface. So far there have been no conclusive answers to shed light on the search. This study therefore aimed to bridge the gap of cancer therapy. Screening against 49 kinds of cell lines belonging to 11 kinds of solids cancers was performed. Isolation and characterization for approximately 4200 monoclonal antibodies (mAb) was also performed thereafter. Of those mAb 488 clones that turned out to bind to 29 tumor-associated antigens (TAA) were subjected to immunohistochemical (IHC) analyses. Selection of target antigens (Ag) and a potential antibody for cancer therapy was conducted prior to clinical examinations. In order to find predictably effective targets for therapeutic Ab against solid cancers, expression of the Ag on the surface of cancer and normal cells was extensively examined by IHC analyses using fresh cancer specimens resected from patients. In this study, the tendencies of all staining patterns and distribution of the Ab are reported. While all of the TAA appeared to be involved in tumorigenesis, their expression was not restricted to some specific tumor types but rather randomly distributed among various cancers. Some kinds of Ab including anti-epidermal growth factor receptor (EGFR) and anti-human epidermal growth factor receptor 2 (HER2) indicated the frequency of expression in normal cells was generally low. We concluded that identification of 488 mAb and the accumulated results of IHC analyses in this study could be the key for further therapeutic Ab against cancers. The targets that showed cancer-specific expression are expected to be better for therapeutic Ab than the other Ab. Moreover, further investigation into the growth of cancer cell lines using full human IgG form of Ab shows available efficacy in specific cases. (Cancer Sci 2011; 102: 175-181)

show abstract

Isolation of antigen/antibody complexes through organic solvent (ICOS) method

Cited by 21 publications

References 15 publications

Isolation of human Fab antibodies specific for the low-affinity IgE receptor (CD23) by selecting a hierarchical antibody library system against B lymphoblastic IM-9 cells

Isolation of human Fab antibodies specific for the low-affinity IgE receptor (CD23) by selecting a hierarchical antibody library system against B lymphoblastic IM-9 cells

Comprehensive Identification of Tumor-associated Antigens via Isolation of Human Monoclonal Antibodies that may be Therapeutic

Selection and analysis of anti‐cancer antibodies for cancer therapy obtained from antibody phage library

Contact Info

Product

Resources

About