Isolation of Antidiabetic Principle from Fruit Rinds of<i>Punica granatum</i>

Jain, Vishal; Viswanatha, Gollapalle Lakshminarayanashastry; Manohar, D.; Shivaprasad, H. N.

doi:10.1155/2012/147202

Cited by 41 publications

(31 citation statements)

References 32 publications

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“…The fruit was used as a 'blood purifier' and to cure parasitic infections, aphthae (mouth ulcers) and diarrhoea. Isolation of antidiabetic principles from the fruit rinds of P. granatum was also reported (Jain et al 2012). Its flowering part has been recommended in Unani literature as a remedy for diabetes (Ali & Sharma 2006).…”

Section: Please Scroll Down For Articlementioning

confidence: 90%

Triterpenes as uncompetitive inhibitors of α-glucosidase from flowers of Punica granatum L.

Dine

Qiong

Kandil

et al. 2014

Natural Product Research

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The α-glucosidase and maltase inhibitory effects of Punica granatum L. flowers (PGF) were investigated. The methanol extract (PGFMe), n-hexane extract (PGFH), chloroform extract (PGFC) and the remaining water fraction (PGFW) were assayed for their α-glucosidase and maltase inhibitory effects. PGFW showed potent α-glucosidase inhibition with IC₅₀ of 0.8 μg/mL followed by PGFMe (IC₅₀ of 4.0 μg/mL) then PGFC (IC₅₀ of 5.21 μg/mL) in comparison to acarbose (0.9 μM). Due to its selectivity towards α-glucosidase, PGFC was subjected to bioactivity-guided isolation of its main active constituents. Five known compounds (1-5) were identified as β-sitosterol (1), oleanolic acid (2), ursolic acid (3), p-coumaric acid (4) and apigenin (5). Ursolic and oleanolic acids showed potent α-glucosidase inhibition (IC₅₀ of 39.0 and 35.0 μM, respectively), while they did not show significant maltase inhibition. Kinetic study using the double Lineweaver-Burk plot revealed that ursolic acid uncompetitively inhibited α-glucosidase in comparison with acarbose as a competitive inhibitor.

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Section: Please Scroll Down For Articlementioning

confidence: 90%

Triterpenes as uncompetitive inhibitors of α-glucosidase from flowers of Punica granatum L.

Dine

Qiong

Kandil

et al. 2014

Natural Product Research

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“…A recent study by Jain et al [32] has shown the probable mechanism of action of PP. They have isolated and purified a compound Valoneic acid dilactone (VAD) from the methanolic extract of Punica granatum (MEPG).…”

Section: Mechanisms Of Actionmentioning

confidence: 99%

A Review on Antihyperglycemic and Antihepatoprotective Activity of Eco-FriendlyPunica granatumPeel Waste

Middha

Usha²,

Pande

2013

Evidence-Based Complementary and Alternative Medicine

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Over the past decade, pomegranate (Punica granatum) is entitled as a wonder fruit because of its voluminous pharmacological properties. In 1830, P. granatum fruit was first recognized in United States Pharmacopeia; the Philadelphia edition introduced the rind of the fruit, the New York edition the bark of the root and further 1890 edition the stem bark was introduced. There are significant efforts and progress made in establishing the pharmacological mechanisms of peel (pericarp or rind) and the individual constituents responsible for them. This review provides an insight on the phytochemical components that contribute too antihyperglycemic, hepatoprotective, antihyperlipidemic effect, and numerous other effects of wonderful, economic, and eco-friendly pomegranate peel extract (PP).

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“…Interestingly, many plant derived constituents such as aconitine, berberine, piperine, baicalin, vitexin, rutin, apigenin, chrysin, eugenol, ellagic acid, gallic acid, quercetin, kaemferol, abietic acid, and α-terpineol are well proved to possess anticonvulsant activity in various experimental models [ 39 ]. In these lines, some of the these phytocompounds such as gallic acid, ellagic acid, rutin, corilagin, kaempferol, luteolin, myricetin, quercetin, and quercimetrine were previously isolated from P. granatum [ 40 ]; based on these observations, we can conclude that the MLPG is eliciting its anticonvulsant activity by synergestic interaction of some these compounds, through enhanced GABA levels in the brain.…”

Section: Discussionmentioning

confidence: 82%

Evaluation of anti-epileptic activity of leaf extracts of Punica granatum on experimental models of epilepsy in mice

Viswanatha¹,

Venkataranganna

Prasad

et al. 2016

J Intercult Ethnopharmacol

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Objectives:This study was aimed to examine the anti-epileptic activity of leaf extracts of Punica granatum in experimental models of epilepsy in Swiss albino mice.Materials and Methods:Petroleum ether leaf extract of P. granatum (PLPG), methanolic LPG (MLPG), and aqueous LPG (ALPG) extracts of P. granatum leaves was initially evaluated against 6-Hz-induced seizure model; the potent extract was further evaluated against maximal electroshock (MES) and pentylenetetrazole (PTZ)-induced convulsions. Further, the potent extract was evaluated for its influence on Gamma amino butyric acid (GABA) levels in brain, to explore the possible mechanism of action. In addition, the potent extract was subjected to actophotometer test to assess its possible locomotor activity deficit inducing action.Results:In 6-Hz seizure test, the MLPG has alleviated 6-Hz-induced seizures significantly and dose dependently at doses 50, 100, 200, and 400 mg/kg. In contrast, PLPG and ALPG did not show any protection, only high dose of ALPG (400 and 800 mg/kg, p.o.) showed very slight inhibition. Based on these observations, only MLPG was tested in MES and PTZ models. Interestingly, the MLPG (50, 100, 200 and 400 mg/kg) has offered significant and dose-dependent protection against MES (P < 0.01) and PTZ-induced (P < 0.01) seizures in mice. Further, MLPG showed a significant increase in brain GABA levels (P < 0.01) compared to control and showed insignificant change in locomotor activity in all tested doses (100, 200 and 400 mg/kg). Interestingly, higher dose of MLPG (400 mg/kg, p.o.) and Diazepam (5 mg/mg, p.o.) have completely abolished the convulsions in all the anticonvulsant tests.Conclusion:These findings suggest that MLPG possesses significant anticonvulsant property, and one of the possible mechanisms behind the anticonvulsant activity of MLPG may be through enhanced GABA levels in the brain.

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Isolation of Antidiabetic Principle from Fruit Rinds ofPunica granatum

Cited by 41 publications

References 32 publications

Triterpenes as uncompetitive inhibitors of α-glucosidase from flowers of Punica granatum L.

Triterpenes as uncompetitive inhibitors of α-glucosidase from flowers of Punica granatum L.

A Review on Antihyperglycemic and Antihepatoprotective Activity of Eco-FriendlyPunica granatumPeel Waste

Evaluation of anti-epileptic activity of leaf extracts of Punica granatum on experimental models of epilepsy in mice

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