Isolation of Antibacterial Nano-Hydroxyapatite Biomaterial from Waste Buffalo Bone and Its Characterization

Parajuli, Kshama; Malla, Komal Prasad; Panchen, Nicodemus; G.C, Ganga; Adhikari, Rameshwar

doi:10.23939/chcht16.01.133

Cited by 9 publications

(7 citation statements)

References 76 publications

(28 reference statements)

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“…employing CuKα radiation (λ = 0.15406 nm) from 2θ ranging from 10° to 80°. The average crystalline size of the ZnO nanoparticles was estimated from the XRD diffraction peaks by using Debye Scherrer'sequation 18 .…”

Section: Characterizationmentioning

confidence: 99%

Preparation of zinc oxide nanoparticles using Citrus sinensis fruit peel aqueous extract and its antimicrobial study

Lamichhane,

Parajuli,

Pradhanang

2024

Sci World

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The remarkable properties of nanoparticles within the 1 to 100 nm size range have garnered significant attention due to their unique characteristics arising from their small size and high surface-to-volume ratio. In this research, zinc oxide nanoparticles (ZnO NPs) were synthesized using an eco-friendly approach, employing orange fruit peel aqueous extract as a stabilizer as well as biological reducing agent and zinc nitrate hexahydrate as a zinc precursor. This method not only reduces the need for copious amounts of chemicals but also eliminates the use of hazardous substances in the fabrication process, augmenting the antibacterial attributes of the nanoparticles. As synthesized material underwent characterization through X-ray diffraction (XRD) analysis where the prepared material was single phase crystalline ZnO NPs and particle size was measured to be 31.2 nm using Debye-Scherrer’s equation. Energy-dispersive X-ray spectroscopy (EDX) revealed that the sample primarily consisted of 98% zinc with minor impurities such as calcium and potassium in it. Fourier-Transform Infrared (FTIR) analysis exhibited a distinct absorption peak at approximately 550 cm⁻¹, confirming the synthesis of ZnO NPs. The antimicrobial potential of the synthesized ZnO NPs was evaluated against two bacterial strains, Staphylococcus aureus and Escherichia coli, through the Kirby Bauer method on media conducive to bacterial growth. The prepared nanoparticles exhibited bactericidal activity, effectively inhibiting bacterial growth, and generating discernible zones of inhibition.

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Section: Characterizationmentioning

confidence: 99%

Preparation of zinc oxide nanoparticles using Citrus sinensis fruit peel aqueous extract and its antimicrobial study

Lamichhane,

Parajuli,

Pradhanang

2024

Sci World

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“…The bone powder was then hydrolyzed by 4M NaOH with 1:40 (Solid: Liquid) at 250 °C for 4-5 hours. The neutral deproteinized and defatted bone powder was then calcined at 900 o C for 5 hours in a muffle furnace to obtain white powder of HA (Barakat et al, 2009, Parajuli et al, 2022. The extraction process photographs are summarized in figure 1 below.…”

Section: Extraction Of Hamentioning

confidence: 99%

Facile Extraction of Natural Hydroxyapatite (HA) from Biowaste and Its Characterizations

Parajuli,

Panchen,

Gautam

2023

Pragya Darshan

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Natural Hydroxyapatite (HA) with chemical formula Ca10(PO4)6(OH)2 is a major mineral storage form of calcium and phosphorous in bone and it has been applied in many fields. In the present study, HA was extracted from biowaste; namely the buffalo bone by alkaline hydrolysis process followed by calcination at 900°C with holding time 6 hrs. in muffle furnace. The isolated material was analyzed by X-ray Diffraction (XRD), Fourier Transform Infra-Red Spectroscopy (FTIR) and Field Emission Scanning Electron Microscopy (FESEM) etc. The SEM image suggested the isolated material had rod shaped and agglomerated. FTIR study indicated the phosphate and hydroxide were the major functional groups along with carbonate present in it. Further, XRD analysis inferred that the isolated material was only HA phased. The specific surface area of the isolated HA was determined by methylene blue adsorption method and it was found to be 141.12 m2 /g and pH point of zero charge (pHPZC) of the extracted biomaterial was determined to be 6.8 by pH the drift method. Thus the pure HA powder was synthesized by an environmentally friendly, cost effective method and may be applicable in many fields including waste water treatment and biomedicine etc.

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“…Natural bone comprises hydroxyapatite (HAp), an inorganic calcium phosphate (CaP) mineral that is primarily embedded in the collagen matrix [1]. It is one of the main parts of vertebrate bones and other mineralized tissues [1][2][3][4][5]. The nano-HAp is well known for its excellent biomedical properties such as osteoconductivity, osteoinductivity, and biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

Acute Oral Toxicity Analysis of Nano-Hydroxyapatite-Gelatin Suspension in Albino Wistar Rats

Malla,

Pandit

et al. 2024

J. Nepal Chem. Soc.

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Hydroxyapatite (HAp), a calcium phosphate-based bio-ceramic, was prepared from waste ostrich bone using the thermal decomposition method, and the nano-HAp was segregated by the water-in-oil microemulsion technique. A single acute dose of oral toxicity of nano-HAp in gelatin suspension was tested in eleven female Albino Wistar rats following the OECD 420 guidelines. The rats were divided into three groups: three for control, three for group I, and five for group II. The first group was given 300 mg of nano-HAp in gelatin suspension per kg of body weight, while group II was given 2000 mg per kg of body weight. Results show that no signs or symptoms of toxic effects were seen in the group during the 14-day study period. Furthermore, no significant change in their average body weight or other physical behaviors such as autonomic, respiratory, or somatomotor effects were observed in the rats. The macroscopic examination of internal organs and bodyweight observation have shown no symptoms of toxicity in either group. It could be concluded that the nano-HAp suspension in gelatin does not show any acute toxic effect. The lethal 50% dose (LD50) of the nano-HAp-Gel suspension has been estimated to be more than 2000 mg/kg of the body weight, suggesting that nano-HAp extracted from ostrich bone is safe to use for calcium supplements and other biomedical applications.

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Isolation of Antibacterial Nano-Hydroxyapatite Biomaterial from Waste Buffalo Bone and Its Characterization

Cited by 9 publications

References 76 publications

Preparation of zinc oxide nanoparticles using Citrus sinensis fruit peel aqueous extract and its antimicrobial study

Preparation of zinc oxide nanoparticles using Citrus sinensis fruit peel aqueous extract and its antimicrobial study

Facile Extraction of Natural Hydroxyapatite (HA) from Biowaste and Its Characterizations

Acute Oral Toxicity Analysis of Nano-Hydroxyapatite-Gelatin Suspension in Albino Wistar Rats

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